Heterologous Protein Expression in Pichia pastoris: Latest Research Progress and Applications

ChemBioChem - Tập 19 Số 1 - Trang 7-21 - 2018
Veeresh Juturu1, Jin Wu1
1Institute of Chemical and Engineering Sciences, Agency for Science, Technology and Research (A*STAR), 1 Pesek Road, Jurong Island, Singapore 627833, Singapore

Tóm tắt

Abstract

Pichia pastoris is a well‐known platform strain for heterologous protein expression. Over the past five years, different strategies to improve the efficiency of recombinant protein expression by this yeast strain have been developed; these include a patent‐free protein expression kit, construction of the P. pastoris CBS7435Ku70 platform strain with its high efficiency in site‐specific recombination of plasmid DNA into the genomic DNA, the design of synthetic promoters and their variants by combining different core promoters with multiple putative transcription factors, the generation of mutant GAP promoter variants with various promoter strengths, codon optimization, engineering the α‐factor signal sequence by replacing the native glutamic acid at the Kex2 cleavage site with the other 19 natural amino acids and the addition of mammalian signal sequence to the yeast signal sequence, and the co‐expression of single chaperones, multiple chaperones or helper proteins that aid in recombinant protein folding. Publically available high‐quality genome data from multiple strains of P. pastoris GS115, DSMZ 70382, and CBS7435 and the continuous development of yeast expression kits have successfully promoted the metabolic engineering of this strain to produce carotenoids, xanthophylls, nootkatone, ricinoleic acid, dammarenediol‐II, and hyaluronic acid. The cell‐surface display of enzymes has obviously increased enzyme stability, and high‐level intracellular expression of acyl‐CoA and ethanol O‐acyltransferase, lipase and d‐amino acid oxidase has opened up applications in whole‐cell biocatalysis for producing flavor molecules and biodiesel, as well as the deracemization of racemic amino acids. High‐level expression of various food‐grade enzymes, cellulases, and hemicellulases for applications in the food, feed and biorefinery industries is in its infancy and needs strengthening.

Từ khóa


Tài liệu tham khảo

10.1073/pnas.69.8.2110

10.1073/pnas.70.11.3240

 

10.1038/nbt.2794

www.marketsandmarkets.com/Market-Reports/protein-expression-market-180323924.html.

10.1016/j.biotechadv.2011.11.006

Liu Z., 2012, BioEngineering, 109, 1259

E. H.Wegner(Phillips Petroleum Company) US 4617274 A 1986.

10.1111/j.1574-6976.2000.tb00532.x

10.1006/prep.1997.0765

10.1038/nrmicro1087

10.1007/978-1-4939-2760-9_8

10.1128/MCB.5.12.3376

10.1186/1475-2859-8-29

10.1186/1475-2859-9-50

10.1021/sb400091p

10.1186/1475-2859-5-39

10.1186/1475-2859-11-22

10.1093/nar/15.9.3859

10.1128/MCB.9.3.1316

J. M.Cregg(Phillips Petroleum Company) US 5032516 A 1991.

10.1016/S1389-1723(01)80321-2

10.1016/S0378-1119(96)00675-0

10.1002/(SICI)1097-0061(19980615)14:8<783::AID-YEA272>3.0.CO;2-Y

10.1016/S0378-1119(98)00315-1

J. M.Cregg(Research Corporation Technologies Inc.) US 6730499 B1 2004.

J. M.Cregg(Research Corporation Technologies Inc.) US 7745200 B2 2010.

10.1002/yea.1028

J.Menendez Diaz I.Valdes Prado N.Cabrera Leon US 20040106783 A1 2004.

10.1074/jbc.270.18.10940

H. W.Lee J. O.Ahn J. K.Jung E. S.Choi C. S.Kim E. G.Lee J. Y.Hong H. W.Lee M. S.Park(Korea Research Institute of Bioscience and Biotechnology) US 8080389 B2 2011.

10.1016/j.jbiotec.2010.09.957

10.1021/acssynbio.5b00199

10.1016/S0378-1119(00)00576-X

10.1371/journal.pone.0039720

10.1111/j.1574-6968.2006.00427.x

10.1016/j.jbiotec.2011.04.014

10.1016/j.femsyr.2004.06.016

10.1371/journal.pone.0068325

10.2174/187220809789389126

10.1093/glycob/cwn044

10.1186/1475-2859-8-53

10.1038/nbt.1544

10.1016/j.ymben.2014.04.001

10.1016/j.jbiotec.2015.10.005

 

www.marketsandmarkets.com/Market-Reports/carotenoid-market-158421566.html;

10.1016/S1074-5521(03)00103-0

10.1016/j.procbio.2009.05.012

10.1007/s00253-011-3764-7

10.1186/2191-0855-2-24

10.1016/j.jbiotec.2014.05.018

10.1194/jlr.M060954

10.1007/s00253-014-5732-5

http://gcua.schoedl.de/.

10.1002/biot.201000332

Wang J. R., 2015, Biomed. Res. Int., 248680

10.1016/j.molcatb.2012.02.009

10.1038/nrm4073

10.1080/21655979.2015.1011031

10.1002/bit.24990

10.1007/s10529-014-1620-4

10.1128/AEM.02843-10

10.1371/journal.pone.0033279

Berg L., 2013, BioMed Res. Int., 926985

F.Hartner A.Glieder(Technische Universität Graz VTU Holding GmbH) US 9012175 B2 2008.

10.1093/nar/gkn369

H.Ohi M.Miura S.Uno M.Chuganji R.Hiramatsu T.Ohmura(The Green Cross Corporation) US 5683893 1997.

H.Ohi M.Miura S.Uno R.Hiramatsu T.Ohmura(The Green Cross Corporation) US 5707827 1998.

10.1371/journal.pone.0075347

M. D.-T.Chang C. C.Sheu(Simpson Biotech Co. Ltd.) US 7785872 B2 2010.

10.1371/journal.pone.0141454

10.4014/jmb.1402.02034

De AmorimAraújo J., 2015, AMB Express., 5, 84, 10.1186/s13568-015-0170-z

10.1111/j.1567-1364.2010.00669.x

X.Chen Z.Huang B.Zhou M.Xu CN 104046645 A 2014.

Chen X., 2015, Turk. J. Biol., 39, 312, 10.3906/biy-1408-33

X.Xu H.Wang D.Li Wanghai CN 104152484 A 2014.

B.Gasser D.Mattanovich M.Sauer G.Stadlmayr(Polymun Scientific Immunbiologische Forschung GmbH) US 20100297738 A1 2010.

10.1186/s40064-015-1195-0

10.1016/j.biortech.2013.10.037

10.1186/1475-2859-9-24

Alias N. I., 2011, African. J. Biotechnol., 35, 6700

10.1186/1472-6750-9-56

Kirikyali N., 2014, AMB Express., 4, 1, 10.1186/s13568-014-0068-1

10.1186/s12896-015-0135-y

10.1007/s10529-014-1716-x

10.1002/jobm.201300078

10.1007/s11274-012-1176-1

10.1007/s11274-012-1215-y

10.1007/s12010-013-0672-6

10.1186/1475-2859-13-57

Quay D. H. X., 2011, African J. Biotechnol., 10, 2101

10.1186/1475-2859-11-25

10.1186/1475-2859-11-61

 

10.1016/j.yrtph.2006.02.001

www.fda.gov/Food/IngredientsPackagingLabeling/GRAS/NoticeInventory/ucm153734.htm.

K.Okabayashi T.Ohmura K.Yokoyama H.Kawabe(The Green Cross Corporation) US 5643792 A 1997.

10.1016/j.biortech.2012.10.042

10.1007/s00253-014-5755-y

T. U.Gerngross(Glycofi Inc.) US 7029872 B2 2006.

T. U.Gerngross S.Wildt B.-K.Choi J. H.Nett P.Bobrowicz S. R.Hamilton R. C.Davidson(Glycofi Inc.) US 7449308 B2 2008.

R.Contreras N. L. M.Callewaert S. C. J.Geysens(Flanders Interuniversity Institute For Biotechnology) US 6803225 B2 2004.

R.Contreras N. L. M.Callewaert S. C. J.Geysens V.Kaigorodov W.Vervecken(Flanders Interuniversity Institute For Biotechnology) US 7252933 B2 2007.

T. U.Gerngross(Glycofi Inc.) US 7326681 B2 2008.

R.Contreras N. L. M.Callewaert W.Vervecken V.Kaigorodov(Research Corporation Technologies Inc.) US 7507573 B2 2009.

S. R.Hamilton (Glycofi Inc.) US 2006286637 A1 2006.

M.Miura M.Hirose T.Miwa H.Irie S.Kuwae K.Miyano W.Otani H.Ohi(Mitsubishi Pharma Corporation) US 7198921 B2 2007.

M.Miura M.Hirose T.Miwa H.Irie S.Kuwae K.Miyano W.Otani H.Ohi(Mitsubishi Tanabe Pharma Corporation) US 7488591 B2 2009.

P.Bobrowicz W. J.Cook W.Kett(Merck Sharp & Dohme Corp.) US 8795984 B2 2014.

Thông tin
Thông tin xuất bản

ChemBioChem

Tập 19 Số 1

7-21

Thông tin tác giả