Biotechnology Techniques

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Production of galactooligosaccharides by β-galactosidase immobilized on glutaraldehyde-treated chitosan beads
Biotechnology Techniques - Tập 12 - Trang 273-276 - 1998
Dey-Chyi Sheu, Shin-Yi Li, Kow-Jen Duan, C. Will Chen
Efficient Transformation of the Astaxanthin-Producing Yeast Phaffia Rhodozyma
Biotechnology Techniques - Tập 12 - Trang 399-405 - 1998
Jan Wery, Jan C. Verdoes, Albert J.J. van Ooyen
An efficient transformation system for the astaxanthin-producing yeast Phaffia rhodozyma was developed based on electroporation that routinely yields approximately 1000 transformants per μg of plasmid DNA. The high transformation efficiency depends on vector integration in the ribosomal DNA (rDNA) and the presence of the homologous glycolytic glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter... hiện toàn bộ
Nonthermal killing effect of microwave irradiation
Biotechnology Techniques - Tập 10 - Trang 145-150 - 1996
Seigo Sato, Chokichiro Shibata, Minoru Yazu
Suspensions of E. coli were exposed to microwave irradiation at constant temperatures. No cell death was observed at 35°C, whereas, at 45, 47, and 50°C, the death rates of E. coli exposed to microwave irradiation were higher than those obtained in conventional heat sterilization at the same temperatures.
Agitation effects on microcarrier and suspension CHO cells
Biotechnology Techniques - Tập 6 - Trang 323-328 - 1992
Kim C. O’Connor, E. Terry Papoutsakis
The growth properties of attachment-dependent and attachment-independent CHO cells cultured in spinner-flask bioreactors were compared to investigate cell damage from rapid agitation. Damage was attributed to bulk-fluid turbulence for attachment-dependent CHO cells and bubble breakup associated with vortex formation for attachment-independent CHO cells. Radiolabeling demonstrated that cell damage ... hiện toàn bộ
Estimation of specific growth rate from agitation speed in DO-stat culture
Biotechnology Techniques - Tập 10 - Trang 303-308 - 1996
Tae Ho Lee, Yong Keun Chang, Bong Hyun Chung
A simple and effective method to estimate the specific growth rate estimation has been developed based on the observation of time changes in the agitation speed in dissolved oxygen(DO)-stat cultures of Brevibacterium ketoglutamicum. The estimation was compared with that using carbon dioxide evolution rate (CER). Estimated values of specific growth rates by both methods agreed well with the data di... hiện toàn bộ
Chiết xuất phản ứng axit lactic bằng các chất chiết xuất amine ba mạch tổng hợp Dịch bởi AI
Biotechnology Techniques - Tập 13 - Trang 915-918 - 1999
Yeon Ki Hong, Won Hi Hong
Hỗn hợp tripropylamine (TPA) và trioctylamine (TOA) hòa tan trong 1-octanol/n-heptane được sử dụng trong quá trình chiết xuất phản ứng axit lactic (L+) trong dung dịch nước. Hệ số phân phối tối đa đạt được trong khoảng tỷ lệ trọng lượng từ 6:4 đến 8:2 của TPA/TOA trong pha nước có 5% (w/w) axit lactic và hiệu suất chiết xuất của chúng vượt quá 90%. Bằng cách đưa TPA vào TOA, sự hình thành pha thứ ... hiện toàn bộ
#chiết xuất phản ứng #axit lactic #amine ba mạch #hệ số phân phối #hiệu suất chiết xuất
An algorithm for adaptive control of dissolved oxygen concentration in batch culture
Biotechnology Techniques - - 1995
Donatas Levišauskas
An effective automatic control algorithm for set-point control of dissolved oxygen concentration in batch culture has been developed. Adaptation of PI controller to the variable state of batch culture is based on analytically obtained functional relations between the controller parameters and the state variables: oxygen uptake rate, stirring speed, and saturation value of dissolved oxygen concentr... hiện toàn bộ
Improved molecular weight analysis of streptococcal hyaluronic acid by size exclusion chromatography
Biotechnology Techniques - Tập 9 - Trang 491-496 - 1995
David C. Armstrong, Michael R. Johns
Four size exclusion chromatography (SEC) calibration techniques were tested for use in the molecular weight characterisation of Streptococcal Hyaluronic Acid (HA). An exponential equation, evaluated using the Hamielec method, was superior to the customary peak position method. It provided the most accurate estimates of the weight average molecular weight, Mw. The calibration was valid for HA in th... hiện toàn bộ
Characterization of a new gas-liquid contactor for the biological treatment of gaseous industrial effluents
Biotechnology Techniques - Tập 10 - Trang 755-760 - 1996
G. M. de Billerbeck, C. Fonade
This paper deals with a new type of gas-liquid contactor for the biological treatment of volatile organic compounds (VOCs). The hydrodynamic and the mass transfer characteristics of this contactor are studied. With head losses quite adapted to the use of blowing engines, the mass transfer capacity is found to be much higher than the mass flowrates which are normally contained in the industrial eff... hiện toàn bộ
Rapid procedure for isolation and detection of Salmonella plasmids
Biotechnology Techniques - Tập 12 - Trang 769-772 - 1998
Martie L. van der Walt, Louise Maré, Marion Bezuidenhout, Anne Barnard, Badie Nieuwoudt
A rapid procedure is described for the isolation of high quality and quantity plasmid DNA from Salmonella. Plasmids of molecular mass ranging from 10 kbp to 147 kbp were isolated. The method yielded enough DNA from the low copy number virulence plasmids of various Salmonella serovars for visualization on an agarose gel. The method is based on the classic alkaline lysis methods, employing the same ... hiện toàn bộ
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