Rapid procedure for isolation and detection of Salmonella plasmids

Biotechnology Techniques - Tập 12 - Trang 769-772 - 1998
Martie L. van der Walt1, Louise Maré1, Marion Bezuidenhout1, Anne Barnard1, Badie Nieuwoudt1
1Salmonella Laboratory, Onderstepoort Veterinary Institute, Private Bag Onderstepoort, South-Africa

Tóm tắt

A rapid procedure is described for the isolation of high quality and quantity plasmid DNA from Salmonella. Plasmids of molecular mass ranging from 10 kbp to 147 kbp were isolated. The method yielded enough DNA from the low copy number virulence plasmids of various Salmonella serovars for visualization on an agarose gel. The method is based on the classic alkaline lysis methods, employing the same reagents, but without any incubation steps. Isolation of plasmid DNA was also attempted with the use of rapid DNA isolation kits, but without success. It was reasoned that endonucleases, present in the resin, might have degraded the plasmids. © Rapid Science Ltd. 1998

Tài liệu tham khảo

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