Agitation effects on microcarrier and suspension CHO cells
Tóm tắt
The growth properties of attachment-dependent and attachment-independent CHO cells cultured in spinner-flask bioreactors were compared to investigate cell damage from rapid agitation. Damage was attributed to bulk-fluid turbulence for attachment-dependent CHO cells and bubble breakup associated with vortex formation for attachment-independent CHO cells. Radiolabeling demonstrated that cell damage included intrinsic changes in DNA synthesis.
Tài liệu tham khảo
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