Estimation of specific growth rate from agitation speed in DO-stat culture

Biotechnology Techniques - Tập 10 - Trang 303-308 - 1996
Tae Ho Lee1, Yong Keun Chang1, Bong Hyun Chung2
1Department of Chemical Engineering and Bioprocess Engineering Research Center, Korea Advanced Institute of Science and Technology, Taejon, Korea
2Korea Research Institute of Biotechnology and Bioengineering, Taejon, Korea

Tóm tắt

A simple and effective method to estimate the specific growth rate estimation has been developed based on the observation of time changes in the agitation speed in dissolved oxygen(DO)-stat cultures of Brevibacterium ketoglutamicum. The estimation was compared with that using carbon dioxide evolution rate (CER). Estimated values of specific growth rates by both methods agreed well with the data directly calculated from cell concentration change although the use of agitation speed gave a slightly better result than CER.

Tài liệu tham khảo

Cardello, R. J. and San, K. Y. (1988). Biotechnol. Bioeng., 32, 519–526. Gauthier, L., Thibault, J., and LeDuy, A. (1991). Biotechnol. Bioeng., 37, 889–893. Konstantinov, K., Kishimoto, M., Seki, T., and Yoshida, T. (1990). Biotechnol. Bioeng., 36, 750–758. Lee, S. C., Kim, C. G., Chang, Y. K., and Chang, H. N. (1992). Proc. ICCAFT5/IFACBIO2, Keystone Colorado, March 29. Riesenberg, D, Menzel, K., Schulz, V., Schulmann, K., Veith, G., Zuber, G., and Knorre, W. A. (1990). Appl. Microbiol. Biotechnol., 34, 77–82. Riesenberg, D, Schulz, V., Knorre W. A., Pohi, H. D., Korz, D., Sanders, E.A., Ross, A., and Deckwer, W.D. (1991). J. Biotechnol., 20, 17–28. Royce P. N. (1992). Biotechnol. Bioeng., 40, 1129–1138. Paalme, T., Tiisma, K., Kahru, A., Vanatalu, K. and Vilu R. (1990). Biotechnol. Bioeng., 35, 312–319. Shimizu, H., Shioya, S., Suga, K. I., and Takamatsu, T. (1989). Appl. Microbiol. Biotechnol., 30, 276–282. Shimizu, H., Arakik, H., S., Shioya, S., and Uage, K. (1991). Biotechnol. Bioeng., 38, 96–205. Wu, W. T., Chen, K. -L., and Chiou, H. -W. (1985). Biotechnol. Bioeng., 27, 756–760.