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BIOspektrum

SCOPUS (2004-2023)

  1868-6249

  0947-0867

 

 

Cơ quản chủ quản:  Elsevier GmbH

Lĩnh vực:
BiotechnologyMolecular Biology

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Red/ET recombination allows for extensive and base-precise manipulations of the Escherichia coli genome. Here we describe how this technology can be applied to improve the performance of the common expression host E. coli BL21(DE3): deletion of the DE3 sequence dispensable for T7-based gene expression enhances strain safety while recovery of the chromosomal phosphogluconolactonase activity prevent...... hiện toàn bộ
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