Stephan Noll, Jan Reyelt, Thomas Rysiok, Roland Kellner, Detlef Güssow, Stefan Jäkel, Stefanie Hager, Harald Kranz
Red/ET recombination allows for extensive and base-precise manipulations of the Escherichia coli genome. Here we describe how this technology can be applied to improve the performance of the common expression host E. coli BL21(DE3): deletion of the DE3 sequence dispensable for T7-based gene expression enhances strain safety while recovery of the chromosomal phosphogluconolactonase activity prevent...... hiện toàn bộ