BIOspektrum
SCOPUS (2004-2023)
1868-6249
0947-0867
Cơ quản chủ quản: Elsevier GmbH
Lĩnh vực:
BiotechnologyMolecular Biology
Các bài báo tiêu biểu
Gezielte Optimierung von Escherichia coli BL21(DE3)
Tập 19 - Trang 211-213 - 2013
Red/ET recombination allows for extensive and base-precise manipulations of the Escherichia coli genome. Here we describe how this technology can be applied to improve the performance of the common expression host E. coli BL21(DE3): deletion of the DE3 sequence dispensable for T7-based gene expression enhances strain safety while recovery of the chromosomal phosphogluconolactonase activity prevent......
Biomethanisierung — ein Prozess zur Ermöglichung der Energiewende?
Tập 20 Số 7 - Trang 816-817 - 2014
Next Generation Sequencing in der mikrobiellen (Meta)Genomforschung
Tập 20 Số 2 - Trang 168-171 - 2014
Chemisch definiert – ein zellbasierter Zytotoxizitätsassay ohne fötales Kälberserum
Tập 23 Số 1 - Trang 61-62 - 2017
Tests for cytotoxicity–respectively biocompatibility–are performed e. g. for biological evaluation of medical devices or during the development of microphysiometric systems. Here, often cell culture methods which involve the use of fetal bovine serum (FBS) are employed. However, using FBS raises scientific and ethical concerns. In the presented work a protocol for a chemically defined test for cyt......