Gezielte Optimierung von Escherichia coli BL21(DE3)

Zhang Y, Buchholz F, Muyrers JP et al. (1998) A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet 20:123–128

Daegelen P, Studier FW, Lenski RE et al. (2009) Tracing ancestors and relatives of Escherichia coli B, and the derivation of B strains REL606 and BL21(DE3). J Mol Biol 394:634–643

Shiloach J, Kaufman J, Guillard AS et al. (1996) Effect of glucose supply strategy on acetate accumulation, growth, and recombinant protein production by Escherichia coli BL21 (lambdaDE3) and Escherichia coli JM109. Biotechnol Bioeng 49:421–428

Studier FW, Moffatt B (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189:113–130

Shuman S (1989) Vaccinia DNA topoisomerase I promotes illegitimate recombination in Escherichia coli. Proc Natl Acad Sci USA 86:3489–3493

Miclet E, Stoven V, Michels PAM et al. (2001) NMR spectroscopic analysis of the first two steps of the pentose-phosphate pathway elucidates the role of 6-phosphogluconolactonase. J Biol Chem 276:34840–34846

Aon JC, Caimi RJ, Taylor AH et al. (2008) Suppressing posttranslational gluconoylation of heterologous proteins by metabolic engineering of Escherichia coli. Appl Environ Microbiol 74:950–958

Studier FW, Daegelen P, Lenski RE et al. (2009) Understanding the differences between genome sequences of Escherichia coli B strains REL606 and BL21(DE3) and comparison of the E. coli B and K-12 Genomes. J Mol Biol 394:653–680

Kupor SR, Fraekel DG (1969) 6-Phosphogluconolactonase mutants of Escherichia coli and a maltose blue gene. J Bacteriol 100:1296–1301

Zimenkov D, Gulevich A, Skorokhodova A et al. (2005) Escherichia coli ORF ybhE is pgl gene encoding 6-phosphogluconolactonase (EC 3.1.1.31) that has no homology with known 6PGLs from other organisms. FEMS Microbiol Lett 244:275–280