Stammcharakterisierung mittels on-line-Redesign von Experimenten
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Neubauer P, Cruz N, Glauche F et al. (2013) Consistent development of bioprocesses from microliter cultures to the industrial scale. Eng Life Sci 13:224–238
Krause M, Neubauer A, Neubauer P (2016) The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli. Microb Cell Fact 15:1–13
Siurkus J, Panula-Perälä J, Horn U et al. (2010) Novel approach of high cell density recombinant bioprocess development: optimisation and scale-up from microliter to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures. Microb Cell Fact 9:1–17
Betts JI, Doig SD, Baganz F (2006) Characterization and application of a miniature 10 mL stirred-tank bioreactor, showing scale-down equivalence with a conventional 7 L reactor. Biotechnol Prog 22:681–688
Glauche F, Pilarek M, Cruz-Bournazou NM et al. (2017) Design of experiments-based high-throughput strategy for development and optimization of efficient cell disruption protocols. Eng Life Sci 17:1166–1172
Neubauer P, Glauche F, Cruz-Bournazou NM (2017) Editorial: Bioprocess development in the era of digitalization. Eng Life Sci 17:1140–1141
Nickel DB, Cruz-Bournazou NM, Wilms T et al. (2016) Online bioprocess data generation, analysis and optimization for parallel fed-batch fermentations in milliliter scale. Eng Life Sci 17:1195–1201