▪ Abstract Measurements of trajectories of individual proteins or lipids in the plasma membrane of cells show a variety of types of motion. Brownian motion is observed, but many of the particles undergo non-Brownian motion, including directed motion, confined motion, and anomalous diffusion. The variety of motion leads to significant effects on the kinetics of reactions among membrane-bou...... hiện toàn bộ
▪ Abstract Stably folded membrane proteins reside in a free energy minimum determined by the interactions of the peptide chains with each other, the lipid bilayer hydrocarbon core, the bilayer interface, and with water. The prediction of three-dimensional structure from sequence requires a detailed understanding of these interactions. Progress toward this objective is summarized in this ...... hiện toàn bộ
▪ Abstract Lipid raft microdomains were conceived as part of a mechanism for the intracellular trafficking of lipids and lipid-anchored proteins. The raft hypothesis is based on the behavior of defined lipid mixtures in liposomes and other model membranes. Experiments in these well-characterized systems led to operational definitions for lipid rafts in cell membranes. These definitions, d...... hiện toàn bộ
Nước là yếu tố thiết yếu cho sự sống theo nhiều cách, và nếu không có nó, các phân tử sinh học có thể không còn thực sự là các phân tử sinh học. Đặc biệt, nước đóng vai trò quan trọng đối với cấu trúc, tính ổn định, động lực học và chức năng của các đại phân tử sinh học. Trong quá trình gập protein, nước trung gian hóa việc co rút chuỗi và tìm kiếm hình thái tự nhiên thông qua một cảnh qu...... hiện toàn bộ
This review summarizes what is known about the biochemical and biophysical mechanisms that initiate the assembly of actin filaments in cells. Assembly and disassembly of these filaments contribute to many types of cellular movements. Numerous proteins regulate actin assembly, but Arp2/3 complex and formins are the focus of this review because more is known about them than other proteins th...... hiện toàn bộ
▪ Abstract Cys2His2 zinc fingers are one of the most common DNA-binding motifs found in eukaryotic transcription factors. These proteins typically contain several fingers that make tandem contacts along the DNA. Each finger has a conserved ββα structure, and amino acids on the surface of the α-helix contact bases in the major groove. This simple,...... hiện toàn bộ