MEMBRANE PROTEIN FOLDING AND STABILITY: Physical Principles
Tóm tắt
▪ Abstract Stably folded membrane proteins reside in a free energy minimum determined by the interactions of the peptide chains with each other, the lipid bilayer hydrocarbon core, the bilayer interface, and with water. The prediction of three-dimensional structure from sequence requires a detailed understanding of these interactions. Progress toward this objective is summarized in this review by means of a thermodynamic framework for describing membrane protein folding and stability. The framework includes a coherent thermodynamic formalism for determining and describing the energetics of peptide-bilayer interactions and a review of the properties of the environment of membrane proteins—the bilayer milieu. Using a four-step thermodynamic cycle as a guide, advances in three main aspects of membrane protein folding energetics are discussed: protein binding and folding in bilayer interfaces, transmembrane helix insertion, and helix-helix interactions. The concepts of membrane protein stability that emerge provide insights to fundamental issues of protein folding.
Từ khóa
Tài liệu tham khảo
Chu B, 1967, Molecular Forces (based on the Baker lectures of Peter JW Debye).
Fauchère J-L, 1983, Eur. J. Med. Chem. C, 18, 369
Mahanty J, 1976, Dispersion Forces.
Pitzer KS, 1995, Thermodynamics.
Privalov PL. 1992. Physical basis of the stability of the folded conformations of proteins. InProtein Folding, ed. TE Creighton, pp. 83–126. New York: Freeman
Ptitsyn OB. 1992. The molten globule state. See Ref.116, pp. 243–300
Rees DC, Chirino AJ, Kim K-H, Komiya H. 1994. Membrane protein structure and stability: implications of the first crystallographic analyses. See Ref.163, pp. 3–26
Richards FM. 1992. Folded and unfolded proteins: an introduction. See Ref.116, pp. 1–58
Tanford C, 1973, The Hydrophobic Effect: Formation of Micelles and Biological Membranes.
White SH, 1977, Ann. NY Acad. Sci., 303, 243
White SH, Wiener MC. 1995. Determination of the structure of fluid lipid bilayer membranes. InPermeability and Stability of Lipid Bilayers, ed. EA Disalvo, SA Simon, pp. 1–19. Boca Raton, FL: CRC Press