A joint PCR-based gene-targeting method using electroporation in the pathogenic fungus Trichosporon asahiiAMB Express - Tập 12 - Trang 1-11 - 2022
Yasuhiko Matsumoto, Tae Nagamachi, Asami Yoshikawa, Tsuyoshi Yamada, Takashi Sugita
Trichosporon asahii is a pathogenic fungus that causes deep-seated fungal infections in immunocompromised patients. Established methods for generating gene-deficient T. asahii mutants exist, but the frequency of obtaining transformants by electroporation remains low. In the present study, we optimized the conditions for gene transfer by electroporation using a ku70 gene-deficient mutant with high ...... hiện toàn bộ
Preparation of UiO-66 loaded Letrozole nano-drug delivery system: enhanced anticancer and apoptosis activityAMB Express - Tập 14 Số 1
Maryam Ronaghi, Ramtin Hajibeygi, Reza Ghodsi, Akram Eidi, Ronak Bakhtiari
AbstractThe use of drug delivery systems in targeting and achieving the targeting of drugs in treating diseases, especially cancer, has attracted the attention of researchers. Letrozole is one of the drugs for the treatment of breast cancer. In this study, the organic-metallic pharmaceutical porous nanostructure based on zirconium UiO-66 loaded letrozole was synthe...... hiện toàn bộ
Analysis and effect of conventional flasks in shaking culture of Escherichia coliAMB Express - Tập 10 - Trang 1-6 - 2020
Masato Takahashi, Hideki Aoyagi
The circulation direct monitoring and sampling system (CDMSS) is used as a monitoring device for CO2 and O2 concentrations of bypass type in shake-culture flask. The CDMSS could measure kLa, an index for evaluating the performance of aerobic culture incubators, and kG, an indicator of the degree of CO2 ventilation in the flask gas phase. We observed that cylindrical flasks provided a different cul...... hiện toàn bộ
Rapid detection of Escherichia coli in beverages using genetically engineered bacteriophage T7AMB Express - Tập 9 - Trang 1-14 - 2019
Nicharee Wisuthiphaet, Xu Yang, Glenn M. Young, Nitin Nitin
Foodborne illness due to bacterial contamination is a significant issue impacting public health that demands new technology which is practical to implement by food industry. Detection of bacteria in food products and production facilities is a crucial strategy supporting food safety assessments. Bacteriophages were investigated as a tool for bacterial detection due to their ability to infect speci...... hiện toàn bộ
Response of microbial community structure and metabolic profile to shifts of inlet VOCs in a gas-phase biofilterAMB Express - Tập 8 - Trang 1-8 - 2018
Lichao Lu, Guangchun Wang, Marvin Yeung, Jinying Xi, Hong-Ying Hu
The effects of inlet VOCs (Volatile Organic Compounds) shifts on microbial community structure in a biofiltration system were investigated. A lab-scale biofilter was set up to treat eight VOCs sequentially. Short declines in removal efficiency appeared after VOCs shifts and then later recovered. The number of OTUs in the biofilter declined from 690 to 312 over time. At the phylum level, Actinobact...... hiện toàn bộ