Randomized controlled trial of oral glutathione supplementation on body stores of glutathione

Springer Science and Business Media LLC - Tập 54 - Trang 251-263 - 2014
John P. Richie1, Sailendra Nichenametla1,2, Wanda Neidig3, Ana Calcagnotto1, Jeremy S. Haley4, Todd D. Schell4, Joshua E. Muscat1
1Department of Public Health Sciences, Penn State Cancer Institute, H069, Penn State University College of Medicine, Hershey, USA
2Orentreich Foundation for the Advancement of Science, Cold Spring, USA
3Penn State Hershey Cancer Institute Clinical Trials Office, Penn State University College of Medicine, Hershey, USA
4Department of Microbiology and Immunology, Penn State University College of Medicine, Hershey, USA

Tóm tắt

Glutathione (GSH), the most abundant endogenous antioxidant, is a critical regulator of oxidative stress and immune function. While oral GSH has been shown to be bioavailable in laboratory animal models, its efficacy in humans has not been established. Our objective was to determine the long-term effectiveness of oral GSH supplementation on body stores of GSH in healthy adults. A 6-month randomized, double-blinded, placebo-controlled trial of oral GSH (250 or 1,000 mg/day) on GSH levels in blood, erythrocytes, plasma, lymphocytes and exfoliated buccal mucosal cells was conducted in 54 non-smoking adults. Secondary outcomes on a subset of subjects included a battery of immune markers. GSH levels in blood increased after 1, 3 and 6 months versus baseline at both doses. At 6 months, mean GSH levels increased 30–35 % in erythrocytes, plasma and lymphocytes and 260 % in buccal cells in the high-dose group (P < 0.05). GSH levels increased 17 and 29 % in blood and erythrocytes, respectively, in the low-dose group (P < 0.05). In most cases, the increases were dose and time dependent, and levels returned to baseline after a 1-month washout period. A reduction in oxidative stress in both GSH dose groups was indicated by decreases in the oxidized to reduced glutathione ratio in whole blood after 6 months. Natural killer cytotoxicity increased >twofold in the high-dose group versus placebo (P < 0.05) at 3 months. These findings show, for the first time, that daily consumption of GSH supplements was effective at increasing body compartment stores of GSH.

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Springer Science and Business Media LLC

Tập 54

251-263

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