Exclusively NOESY-based automated NMR assignment and structure determination of proteins

Journal of Biomolecular NMR - Tập 50 - Trang 137-146 - 2011
Teppei Ikeya1,2, Jun-Goo Jee2, Yoshiki Shigemitsu2, Junpei Hamatsu2, Masaki Mishima2, Yutaka Ito2, Masatsune Kainosho2,3, Peter Güntert1,2
1Institute of Biophysical Chemistry, Center for Biomolecular Magnetic Resonance, and Frankfurt Institute for Advanced Studies, Goethe University Frankfurt am Main, Frankfurt am Main, Germany
2Graduate School of Science, Tokyo Metropolitan University, Hachioji, Japan
3Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya, Japan

Tóm tắt

A fully automated method is presented for determining NMR solution structures of proteins using exclusively NOESY spectra as input, obviating the need to measure any spectra only for obtaining resonance assignments but devoid of structural information. Applied to two small proteins, the approach yielded structures that coincided closely with conventionally determined structures.

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Thông tin xuất bản

Journal of Biomolecular NMR

Tập 50

137-146

Thông tin tác giả