Nuclear Overhauser effects and the assignment of the proton NMR spectra of proteins
Tóm tắt
A systematic procedure is outlined to reveal assignments in the 1H NMR spectrum of a protein that are consistent with a limited set of nuclear Overhauser effect data and with specific assumptions. The results of applying this to a group of resonances in the spectrum of hen lysozyme, most of which were previously assigned by independent methods, are described. The number of possible assignment schemes obtained depends on the distance constraints chosen for the Overhauser effect data, and the investigation of this dependence is proposed as a method of assessing the confidence that can be placed in individual assignments obtained in this way.
Tài liệu tham khảo
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