Yeast
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Characterization of Ccw12p, a major key player in cell wall stability of Saccharomyces cerevisiae
Yeast - Tập 24 Số 4 - Trang 309-319 - 2007
AbstractThe GPI‐anchored mannoprotein Ccw12p is a crucial structural component
of the cell wall of Saccharomyces cerevisiae. Compared to wild‐type, the mutant
ccw12Δ grows more slowly, is highly sensitive to Calcofluor white and contains
2.5 times more cell wall chitin. In this study, electron microscopy of ccw12Δ
cell walls revealed that, with respect to wild‐type, the inner glucan layer is
thick...
Yeast/E. coli shuttle vectors with multiple unique restriction sites
Yeast - Tập 2 Số 3 - Trang 163-167 - 1986
AbstractTwo yeast/E. coli shuttle vectors have been constructed. The two
vectors, YEp351 and YEp352, have the following properties: (1) they can
replicate autonomuosly in Saccharomyces cerevisiae and in E. coli; (2) they
contain the β‐lactamase gene and confer ampicillin resistance to E. coli; (3)
they contain the entire sequence of pUC18; (4) all ten restriction sites of the
multiple cloning regi...
Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure
Yeast - Tập 11 Số 4 - Trang 355-360 - 1995
AbstractAn improved lithium acetate (LiAc)/single‐stranded DNA
(SS‐DNA)/polyethylene glycol (PEG) protocol which yields >1 × 106
transformants/μg plasmid DNA and the original protocol described by Schiestl and
Gietz (1989) were used to investigate aspects of the mechanism of
LiAc/SS‐DNA/PEG transformation. The highest transformation efficiency was
observed when 1 × 108 cells were transformed with ...
Four ARF GAPs in Saccharomyces cerevisiae have both overlapping and distinct functions
Yeast - Tập 20 Số 4 - Trang 315-330 - 2003
AbstractPrevious studies in yeast have revealed the presence of four proteins
with a conserved, cysteine‐rich, ARF GAP domain that share the ability to
suppress the conditional growth defect of the arf1‐3 mutant. Three of these
proteins have been shown previously to be ADP‐ribosylation factor (ARF)
GTPase‐activating proteins (GAPs). We now demonstrate that the fourth also
exhibits in vitro ARF GAP...
Mô-đun dị loại mới cho sự huỷ bỏ gene cổ điển hoặc dựa trên PCR trong Saccharomyces cerevisiae Dịch bởi AI
Yeast - Tập 10 Số 13 - Trang 1793-1808 - 1994
Tóm tắtChúng tôi đã xây dựng và kiểm nghiệm một mô-đun kháng sinh ưu thế, để lựa
chọn các biến đổi gen của S. cerevisiae, hoàn toàn bao gồm DNA dị loại. Mô-đun
kanMX này chứa khung đọc mở kanr đã biết của yếu tố di chuyển Tn903 từ E. coli
kết hợp với các chuỗi điều khiển phiên mã và dịch mã của gene TEF từ nấm sợi
Ashbya gossypii. Mô-đun lai này cho phép lựa chọn hiệu quả các biến đổi gen
kháng lạ...
#Mô-đun kháng dị loại #huỷ bỏ gene #<i>S. cerevisiae</i> #khung đọc mở #PCR #biến đổi gen #kháng geneticin #lựa chọn G418 #phiên mã #dịch mã #nấm sợi <i>Ashbya gossypii</i> #bất hoạt gene #lặp lại trực tiếp #tích hợp đúng #vị trí hạn chế #<i>in vivo</i>.
Sequence of a cytochrome c gene from Kluyveromyces lactis and its upstream region
Yeast - Tập 9 Số 2 - Trang 201-204 - 1993
AbstractThe complete sequence of a cytochrome c gene from Kluyveromyces lactis
including its upstream region is reported. Sequence of the translated open
reading frame is discussed in terms of cytochrome c structural requirements.
Putative regulatory signals in the upstream region are described and compared
with reported sequences which modulate the expression of respiratory‐related
yeast genes.
Cell wall structure suitable for surface display of proteins in Saccharomyces cerevisiae
Yeast - Tập 31 Số 2 - Trang 67-76 - 2014
AbstractA display system for adding new protein functions to the cell surfaces
of microorganisms has been developed, and applications of the system to various
fields have been proposed. With the aim of constructing a cell surface
environment suitable for protein display in Saccharomyces cerevisiae, the cell
surface structures of cell wall mutants were investigated. Four cell wall mutant
strains we...
Three yeast genes, PIR1, PIR2 and PIR3, containing internal tandem repeats, are related to each other, and PIR1 and PIR2 are required for tolerance to heat shock
Yeast - Tập 9 Số 5 - Trang 481-494 - 1993
AbstractWe isolated three highly homologous genes, PIR1, PIR2 and PIR3,
collectively called the PIR genes. The remarkable feature of their putative
amino acid sequence is that they contain a sequence consisting of 18–19 amino
acid residues repeated tandemly seven to ten times. Genes homologous to PIR were
found in Kluyveromyces lactis and Zygosaccharomyces rouxii but not in
Schizosaccharomyces pom...
Isolation of Pichia pastoris PIR genes and their utilization for cell surface display and recombinant protein secretion
Yeast - Tập 28 Số 3 - Trang 213-226 - 2011
AbstractProteins with internal repeats are highly conserved among budding
yeasts. In this study, the isolation of two proteins with internal repeats (PIR)
genes, i.e. PpPIR1 and PpPIR2, from the methylotrophic yeast Pichia pastoris has
been reported. The PIR1 and PIR2 genes' open reading frames were found to
contain 1068 and 972 bases, respectively. The sequence homology search showed a
homologous...
A cell surface display system using novel GPI‐anchored proteins in Hansenula polymorpha
Yeast - Tập 19 Số 13 - Trang 1153-1163 - 2002
AbstractA cell surface display system was developed in yeast Hansenula
polymorpha.The four genes HpSED1, HpGAS1, HpTIP1and HpCWP1, encoding
glycosylphosphatidyl‐inositol (GPI)‐anchored cell surface proteins from H.
polymorpha, were cloned, characterized and evaluated for their efficacies as
cell surface display motifs of reporter proteins. Sequence analysis of these
genes revealed that each encode...
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