Yeast

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Characterization of Ccw12p, a major key player in cell wall stability of Saccharomyces cerevisiae
Yeast - Tập 24 Số 4 - Trang 309-319 - 2007
Enrico Ragni, Matthias Sipiczki, Sabine Strahl
AbstractThe GPI‐anchored mannoprotein Ccw12p is a crucial structural component of the cell wall of Saccharomyces cerevisiae. Compared to wild‐type, the mutant ccw12Δ grows more slowly, is highly sensitive to Calcofluor white and contains 2.5 times more cell wall chitin. In this study, electron microscopy of ... hiện toàn bộ
Yeast/E. coli shuttle vectors with multiple unique restriction sites
Yeast - Tập 2 Số 3 - Trang 163-167 - 1986
James E. Hill, Alan M. Myers, T J Koerner, A Tzagoloff
AbstractTwo yeast/E. coli shuttle vectors have been constructed. The two vectors, YEp351 and YEp352, have the following properties: (1) they can replicate autonomuosly in Saccharomyces cerevisiae and in E. coli; (2) they contain the β‐lactamase gene and confer ampicillin resistance to E. coli... hiện toàn bộ
Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure
Yeast - Tập 11 Số 4 - Trang 355-360 - 1995
R. Daniel Gietz, Robert H. Schiestl, Andrew Willems, Robin A. Woods
AbstractAn improved lithium acetate (LiAc)/single‐stranded DNA (SS‐DNA)/polyethylene glycol (PEG) protocol which yields >1 × 106 transformants/μg plasmid DNA and the original protocol described by Schiestl and Gietz (1989) were used to investigate aspects of the mechanism of LiAc/SS‐DNA/PEG transformation. The highest transformation efficien...... hiện toàn bộ
Four ARF GAPs in Saccharomyces cerevisiae have both overlapping and distinct functions
Yeast - Tập 20 Số 4 - Trang 315-330 - 2003
Chunjiang Zhang, J. Bradford Bowzard, Aimee Alexander, Richard Kahn
AbstractPrevious studies in yeast have revealed the presence of four proteins with a conserved, cysteine‐rich, ARF GAP domain that share the ability to suppress the conditional growth defect of the arf1‐3 mutant. Three of these proteins have been shown previously to be ADP‐ribosylation factor (ARF) GTPase‐activating proteins (GAPs). We no...... hiện toàn bộ
Mô-đun dị loại mới cho sự huỷ bỏ gene cổ điển hoặc dựa trên PCR trong Saccharomyces cerevisiae Dịch bởi AI
Yeast - Tập 10 Số 13 - Trang 1793-1808 - 1994
Achim Wach, Arndt Brachat, Rainer Pöhlmann, Peter Philippsen
Tóm tắtChúng tôi đã xây dựng và kiểm nghiệm một mô-đun kháng sinh ưu thế, để lựa chọn các biến đổi gen của S. cerevisiae, hoàn toàn bao gồm DNA dị loại. Mô-đun kanMX này chứa khung đọc mở kanr đã biết của yếu tố di chuyển Tn903 từ E. coli... hiện toàn bộ
#Mô-đun kháng dị loại #huỷ bỏ gene #<i>S. cerevisiae</i> #khung đọc mở #PCR #biến đổi gen #kháng geneticin #lựa chọn G418 #phiên mã #dịch mã #nấm sợi <i>Ashbya gossypii</i> #bất hoạt gene #lặp lại trực tiếp #tích hợp đúng #vị trí hạn chế #<i>in vivo</i>.
Sequence of a cytochrome c gene from Kluyveromyces lactis and its upstream region
Yeast - Tập 9 Số 2 - Trang 201-204 - 1993
María Ángeles Freire-Picos, Ana María Rodríguez Torres, Elvira Ramil, M. Esperanza Cerdán, Karin D. Breunig, Cornelis P. Hollenberg, Richard S. Zitomer
AbstractThe complete sequence of a cytochrome c gene from Kluyveromyces lactis including its upstream region is reported. Sequence of the translated open reading frame is discussed in terms of cytochrome c structural requirements. Putative regulatory signals in the upstream region are ...
Cell wall structure suitable for surface display of proteins in Saccharomyces cerevisiae
Yeast - Tập 31 Số 2 - Trang 67-76 - 2014
Hiroyuki Matsuoka, Kazuya Hashimoto, Aki Saijo, Yuki Takada, Akihiko Kondo, Mitsuyoshi Ueda, Hiroshi Ooshima, Taro Tachibana, Masayuki Azuma
AbstractA display system for adding new protein functions to the cell surfaces of microorganisms has been developed, and applications of the system to various fields have been proposed. With the aim of constructing a cell surface environment suitable for protein display in Saccharomyces cerevisiae, the cell surface structures of cell wall...... hiện toàn bộ
Three yeast genes, PIR1, PIR2 and PIR3, containing internal tandem repeats, are related to each other, and PIR1 and PIR2 are required for tolerance to heat shock
Yeast - Tập 9 Số 5 - Trang 481-494 - 1993
Akio Toh‐e, Seikoh Yasunaga, H Nisogi, Yasuji Matsui
AbstractWe isolated three highly homologous genes, PIR1, PIR2 and PIR3, collectively called the PIR genes. The remarkable feature of their putative amino acid sequence is that they contain a sequence consisting of 18–19 amino acid residues repeated tandemly seven to ten times. Genes ho...... hiện toàn bộ
Isolation of Pichia pastoris PIR genes and their utilization for cell surface display and recombinant protein secretion
Yeast - Tập 28 Số 3 - Trang 213-226 - 2011
Yogender Pal Khasa, Sara K. Conrad, Mustafa Şengül, Sarah A. Plautz, Michael M. Meagher, Mehmet İnan
AbstractProteins with internal repeats are highly conserved among budding yeasts. In this study, the isolation of two proteins with internal repeats (PIR) genes, i.e. PpPIR1 and ... hiện toàn bộ
A cell surface display system using novel GPI‐anchored proteins in Hansenula polymorpha
Yeast - Tập 19 Số 13 - Trang 1153-1163 - 2002
So‐Young Kim, Jung‐Hoon Sohn, Yu‐Ryang Pyun, Eui‐Sung Choi
AbstractA cell surface display system was developed in yeast Hansenula polymorpha.The four genes HpSED1, HpGAS1, HpTIP1and HpCWP1, encoding glycosylphosphatidyl‐inositol (GPI)‐anchored cell surface proteins from H. poly...... hiện toàn bộ
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