Yeast

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Yeast/E. coli shuttle vectors with multiple unique restriction sites
Yeast - Tập 2 Số 3 - Trang 163-167 - 1986
James E. Hill, Alan M. Myers, T J Koerner, A Tzagoloff
AbstractTwo yeast/E. coli shuttle vectors have been constructed. The two vectors, YEp351 and YEp352, have the following properties: (1) they can replicate autonomuosly in Saccharomyces cerevisiae and in E. coli; (2) they contain the β‐lactamase gene and confer ampicillin resistance to E. coli... hiện toàn bộ
Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure
Yeast - Tập 11 Số 4 - Trang 355-360 - 1995
R. Daniel Gietz, Robert H. Schiestl, Andrew Willems, Robin A. Woods
AbstractAn improved lithium acetate (LiAc)/single‐stranded DNA (SS‐DNA)/polyethylene glycol (PEG) protocol which yields >1 × 106 transformants/μg plasmid DNA and the original protocol described by Schiestl and Gietz (1989) were used to investigate aspects of the mechanism of LiAc/SS‐DNA/PEG transformation. The highest transformation efficien...... hiện toàn bộ
Mô-đun dị loại mới cho sự huỷ bỏ gene cổ điển hoặc dựa trên PCR trong Saccharomyces cerevisiae Dịch bởi AI
Yeast - Tập 10 Số 13 - Trang 1793-1808 - 1994
Achim Wach, Arndt Brachat, Rainer Pöhlmann, Peter Philippsen
Tóm tắtChúng tôi đã xây dựng và kiểm nghiệm một mô-đun kháng sinh ưu thế, để lựa chọn các biến đổi gen của S. cerevisiae, hoàn toàn bao gồm DNA dị loại. Mô-đun kanMX này chứa khung đọc mở kanr đã biết của yếu tố di chuyển Tn903 từ E. coli... hiện toàn bộ
#Mô-đun kháng dị loại #huỷ bỏ gene #<i>S. cerevisiae</i> #khung đọc mở #PCR #biến đổi gen #kháng geneticin #lựa chọn G418 #phiên mã #dịch mã #nấm sợi <i>Ashbya gossypii</i> #bất hoạt gene #lặp lại trực tiếp #tích hợp đúng #vị trí hạn chế #<i>in vivo</i>.
Sequence of a cytochrome c gene from Kluyveromyces lactis and its upstream region
Yeast - Tập 9 Số 2 - Trang 201-204 - 1993
María Ángeles Freire-Picos, Ana María Rodríguez Torres, Elvira Ramil, M. Esperanza Cerdán, Karin D. Breunig, Cornelis P. Hollenberg, Richard S. Zitomer
AbstractThe complete sequence of a cytochrome c gene from Kluyveromyces lactis including its upstream region is reported. Sequence of the translated open reading frame is discussed in terms of cytochrome c structural requirements. Putative regulatory signals in the upstream region are ...
Genome‐wide identification of fungal GPI proteins
Yeast - Tập 20 Số 9 - Trang 781-796 - 2003
Uwe Groß, Klaas J. Hellingwerf, Frans M. Klis
AbstractGlycosylphosphatidylinositol‐modified (GPI) proteins share structural features that allow their identification using a genomic approach. From the known S. cerevisiae and C. albicans GPI proteins, the following consensus sequence for the GPI attachment site and its downstream region was derived: [NSGDAC]–...... hiện toàn bộ
Sequence, mapping and disruption of CCC2, a gene that cross‐complements the Ca2+‐sensitive phenotype of csg1 mutants and encodes a P‐type ATPase belonging to the Cu2+‐ATPase subfamily
Yeast - Tập 11 Số 3 - Trang 283-292 - 1995
D. Fu, Troy Beeler, Thomas M. Dunn
AbstractWe have isolated, sequenced, mapped and disrupted a gene, CCC2, from Saccharomyces cerevisiae. This gene displays non‐allelic complementation of the Ca2+‐sensitive phenotype conferred by the csg1 mutation. Analysis of the CCC2p amino acid sequence reveals t...... hiện toàn bộ
Mass spectrometry‐based profiling of phospholipids and sphingolipids in extracts from Saccharomyces cerevisiae
Yeast - Tập 23 Số 6 - Trang 465-477 - 2006
Xue Li Guan, Markus R. Wenk
AbstractLipids are rapidly moving to centre stage in many fields of biological sciences. Lipidomics, the systems‐level scale analysis of lipids and their interacting factors, is thus an emerging field which holds great promise for drug and biomarker discovery. Here we present a mass spectrometry‐based approach for profiling of polar lipids, in particular phospholip...... hiện toàn bộ
Characterization of Ypr1p from Saccharomyces cerevisiae as a 2‐methylbutyraldehyde reductase
Yeast - Tập 19 Số 12 - Trang 1087-1096 - 2002
Gordon Ford, Elizabeth M. Ellis
AbstractThe metabolism of aldehydes and ketones in yeast is important for biosynthetic, catabolic and detoxication processes. Aldo‐keto reductases are a family of enzymes that are able to reduce aldehydes and ketones. The roles of individual aldo‐keto reductases in yeast has been difficult to determine because of overlapping substrate specificities of these enzymes...... hiện toàn bộ
Associating protein activities with their genes: rapid identification of a gene encoding a methylglyoxal reductase in the yeast Saccharomyces cerevisiae
Yeast - Tập 20 Số 6 - Trang 545-554 - 2003
Ching‐Nen Nathan Chen, L. V. Porubleva, Georgia Shearer, Maja Svrakic, Lauren G. Holden, James L. Dover, Mark Johnston, Parag R. Chitnis, Daniel H. Kohl
AbstractMethylglyoxal is associated with a broad spectrum of biological effects, including cytostatic and cytotoxic activities. It is detoxified by the glyoxylase system or by its reduction to lactaldehyde by methylglyoxal reductase. We show that methylglyoxal reductase (NADPH‐dependent) is encoded by GRE2 (YOL151w... hiện toàn bộ
The role of PaAAC1 encoding a mitochondrial ADP/ATP carrier in the biosynthesis of extracellular glycolipids, mannosylerythritol lipids, in the basidiomycetous yeast Pseudozyma antarctica
Yeast - Tập 27 Số 7 - Trang 379-388 - 2010
Tomotake Morita, Emi Ito, Tokuma Fukuoka, Tomohiro Imura, Daï Kitamoto
AbstractPseudozyma antarctica produces large amounts of the glycolipid biosurfactants known as mannosylerythritol lipids (MEL), which show not only excellent surface‐active properties but also versatile biochemical actions. A gene homologous with a mitochondrial ADP/ATP carrier was dominantly expressed in P. antarctica... hiện toàn bộ
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