The role of PaAAC1 encoding a mitochondrial ADP/ATP carrier in the biosynthesis of extracellular glycolipids, mannosylerythritol lipids, in the basidiomycetous yeast Pseudozyma antarctica

Yeast - Tập 27 Số 7 - Trang 379-388 - 2010
Tomotake Morita1, Emi Ito1, Tokuma Fukuoka1, Tomohiro Imura1, Daï Kitamoto1
1Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Central 5‐2, Higashi 1‐1, Tsukuba, Ibaraki 305‐8565, Japan.

Tóm tắt

AbstractPseudozyma antarctica produces large amounts of the glycolipid biosurfactants known as mannosylerythritol lipids (MEL), which show not only excellent surface‐active properties but also versatile biochemical actions. A gene homologous with a mitochondrial ADP/ATP carrier was dominantly expressed in P. antarctica under MEL‐producing conditions on the basis of previous gene expression analysis. The gene encoding the mitochondrial ADP/ATP carrier of P. antarctica (PaAAC1) contained a putative open reading frame of 954 bp and encodes a polypeptide of 317 amino acids. The deduced translation product shared high identity of 66%, 70%, 69%, 74%, 75% and 52% with the mitochondrial ADP/ATP carrier of Saccharomyces cerevisiae (AAC1), S. cerevisiae (AAC2), S. cerevisiae (AAC3), Kluyveromyces lactis (KlAAC), Neurospora crassa (NcAAC) and human (ANT1), respectively, and conserved the consensus sequences of all ADP/ATP carrier proteins. The gene expression by introducing a plasmid pUXV1‐PaAAC1 into the yeast cells increased the MEL production. In addition, the expression of PaAAC1 in which the conserved arginine and leucine required for ATP transport activity were replaced with isoleucine and serine, respectively, failed to increase MEL production. Accordingly, these results suggest that PaAAC1 encoding a mitochondrial ADP/ATP carrier should be involved in MEL biosynthesis in the yeast. Copyright © 2010 John Wiley & Sons, Ltd.

Từ khóa


Tài liệu tham khảo

10.1093/nar/25.17.3389

10.1002/j.1460-2075.1984.tb01815.x

10.1016/0378-1119(96)00095-9

10.1016/0014-5793(91)81059-H

10.1093/emboj/18.12.3214

10.1016/j.mito.2007.10.001

10.1128/AEM.71.6.3033-3040.2005

10.1021/bi960668j

10.1016/0378-1119(91)90115-R

10.1016/0378-1119(93)90632-D

Kitamoto D, 2002, Functional and potential applications of glycolipid biosurfactants, J Biosci Bioeng, 94, 187, 10.1016/S1389-1723(02)80149-9

10.1023/A:1012464717259

10.1023/A:1005347022247

10.5650/jos1956.42.346

10.1271/bbb1961.54.31

10.1016/j.bbamem.2008.04.011

Kolarov J, 1990, A third ADP/ATP translocator gene in yeast, J Biol Chem, 265, 14195, 10.1016/S0021-9258(19)38402-9

10.1016/0005-2728(72)90174-0

10.1126/science.158.3808.1564

Lawson JE, 1988, Separate genes encode functionally equivalent ADP/ATP carrier proteins in Saccharomyces cerevisiae, J Biol Chem, 263, 14812, 10.1016/S0021-9258(18)68111-6

10.1002/yea.1386

10.1007/s00253-006-0466-7

10.1263/jbb.104.517

10.1042/BA20090033

10.1002/(SICI)1097-0061(19990915)15:12<1237::AID-YEA446>3.0.CO;2-3

O'Malley K, 1982, Selection of the nuclear gene for the mitochondrial adenine nucleotide translocator by genetic complementation of the op1 mutation in yeast, J Biol Chem, 257, 2097, 10.1016/S0021-9258(19)68151-2

10.1016/0005-2760(91)90089-Z

10.1038/nature02056

10.1016/j.bbamcr.2006.08.034

10.1007/s00253-004-1672-9

10.1111/j.1432-1033.1993.tb17793.x

10.1016/0014-5793(82)80648-0

10.1016/S0005-2728(98)00180-7

10.1093/nar/22.22.4673

10.1042/BJ20040650

10.1007/BF00326153

10.1016/0959-440X(92)90081-H

Thông tin
Thông tin xuất bản

Yeast

Tập 27 Số 7

379-388

Thông tin tác giả