Sequence, mapping and disruption of <i>CCC2</i>, a gene that cross‐complements the Ca<sup>2+</sup>‐sensitive phenotype of <i>csg1</i> mutants and encodes a P‐type ATPase belonging to the Cu<sup>2+</sup>‐ATPase subfamily

Yeast - Tập 11 Số 3 - Trang 283-292 - 1995
D. Fu1, Troy Beeler, Thomas M. Dunn
1Department of Biochemistry, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 USA

Tóm tắt

AbstractWe have isolated, sequenced, mapped and disrupted a gene, CCC2, from Saccharomyces cerevisiae. This gene displays non‐allelic complementation of the Ca2+‐sensitive phenotype conferred by the csg1 mutation. Analysis of the CCC2p amino acid sequence reveals that it encodes a member of the P‐type ATPase family and is most similar to a subfamily thought to consist of Cu2+ transporters, including the human genes that mutate to cause Wilson disease and Menkes disease. The ability of this gene, in two or more copies, to reverse the csg1 defect suggests that Ca2+‐induced death of csg1 mutant cells is related to Cu2+ metabolism. Cells without CCC2 require increased Cu2+ concentrations for growth. Therefore CCC2p may function to provide Cu2+ to a cellular compartment rather than in removal of excess of Cu2+. The sequence of CCC2 is available through GenBank under accession number L36317.

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Tài liệu tham khảo

Beeler T., 1994, A novel protein, CSG2p, is required for Ca2+ regulation in S. cerevisiae, J. Biol. Chem., 269, 7279, 10.1016/S0021-9258(17)37280-0

10.1016/0076-6879(87)54076-9

10.1021/bi00286a015

10.1038/ng1293-327

10.1038/ng0193-14

10.1016/S0021-9258(17)37279-4

10.1002/yea.320100411

10.1111/j.1749-6632.1992.tb43788.x

10.1073/pnas.84.10.3112

10.1016/0022-2836(82)90515-0

10.1073/pnas.84.15.5106

10.1016/S0006-3495(90)82482-6

10.1038/ng0193-20

Odermatt A., 1993, Primary structure of two P‐type ATPases involved in copper homeostasis in Enterococcus hirae, J. Biol. Chem., 268, 12775, 10.1016/S0021-9258(18)31455-8

10.1073/pnas.83.20.7826

10.1038/ng1293-338

10.1016/0378-1119(87)90232-0

10.1016/0076-6879(83)01015-0

Sambrook J., 1989, Molecular Cloning. A Laboratory Manual

10.1016/0005-2728(90)90247-2

Sherman F., 1986, Methods in Yeast Genetics

Sikorski R. S., 1989, A system of shuttle vectors and yeast host strains for efficient manipulation of DNA in Saccharomyces cerevisiae, Genetics, 122, 19, 10.1093/genetics/122.1.19

10.1111/j.1365-2958.1993.tb00898.x

10.1038/ng1293-344

10.1038/ng0193-7

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Yeast

Tập 11 Số 3

283-292

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