The Journal of Membrane Biology
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Lipid-Membrane Affinity of Chimeric Metal-binding Green Fluorescent Protein
The Journal of Membrane Biology - Tập 200 - Trang 47-56 - 2004
The Green Fluorescent Protein (GFP) is a useful marker to trace the expression of cellular proteins. However, little is known about changes in protein interaction properties after fusion to GFP. In this study, we present evidence for a binding affinity of chimeric cadmium-binding green fluorescent proteins to lipid membrane. This affinity has been observed in both cellular membranes and artificial......
Role of passive potassium fluxes in cell volume regulation in cultured HeLa cells
The Journal of Membrane Biology - Tập 87 - Trang 93-105 - 1985
Cultured HeLa cells behave as ideal osmometers when subjected to hyperosmolar media, and show no volume regulatory behavior. In hypoosmolar solutions, cell swelling is not as great as predicted, and this is due largely to a loss of intracellular KCl. In hyperosmolar solutions there is a stimulation of the ouabain-insensitive but loop diuretic-sensitive86Rb+ (K+) pathway. Analysis of the K+, Na+ an......
Effect of limited trypsin digestion on the renal Na+−H+ exchanger and its regulation by cAMP-Dependent protein kinase
The Journal of Membrane Biology - Tập 109 - Trang 233-241 - 1989
The Na+−H+ exchanger from solubilized rabbit renal brush border membranes is inhibited by cAMP-dependent protein kinase (PKA) mediated protein phosphorylation. To characterize this inhibitory response and its sensitivity to limited proteolysis, the activity of the transporter was assayed after reconstitution of the proteins into artificial lipid vesicles. Limited trypsin digestion increased the ba......
Reassessment of Models of Facilitated Transport and Cotransport
The Journal of Membrane Biology - Tập 234 - Trang 75-112 - 2010
Most membrane transport models are determinate, requiring the transported ligand(s) to bind initially to a vacant site, which undergoes translation and releases ligand to the alternate side. The carrier reverts to its initial position to complete the net transport cycle. Ligand affinity may change during translation, but this must be compensated by an equivalent energy change(s) within the transpo......
Regulation of excitation-secretion coupling by thyrotropin-releasing hormone (TRH): Evidence for TRH receptor-ion channel coupling in cultured pituitary cells
The Journal of Membrane Biology - Tập 71 - Trang 109-118 - 1983
The electrophysiological and secretory properties of a well-studied clonal line of rat anterior pituitary cells (GH3) have been compared with a new line of morphologically distinct cells derived from it (XG-10). The properties of the latter cells differ from the parent cells in that they do not have receptors for thyrotropin-releasing hormone and their basal rate of secretion is substantially high......
Membrane permeability equations and their solutions for red cells
The Journal of Membrane Biology - Tập 34 - Trang 103-144 - 1977
The mathematical equations for the transport of nonelectrolytes across cell membranes are critically examined and cast in forms suitable for solution which involve fewer approximations than has heretofore been commonly done. For the case of red cells, the equations are developed to include the effect of the variation in apparent nonosmotic water owing to the variation in hemoglobin concentration a......
Oscillations of membrane potential in L cells
The Journal of Membrane Biology - Tập 35 - Trang 337-350 - 1977
Oscillation and activated hyperpolarizing responses induced by electrical stimuli (H.A. responses) were studied in large nondividing L cells (giant L cells) under a variety of ionic conditions. When Cl− in the bathing fluid was partially replaced with SO
4
2−
at fixed external Na+ and K+ concentrations, the membrane potential depolarized transie......
Microelectrode characterization of the basolateral membrane of rabbit S3 proximal tubule
The Journal of Membrane Biology - Tập 108 - Trang 53-60 - 1989
The purpose of this study was to characterize the basolateral membrane of the S3 segment of the rabbit proximal tubule using conventional and ion-selective microelectrodes. When compared with results from S1 and S2 segments, S3 cells under control conditions have a more negative basolateral membrane potential (V
bl=−69 mV), a higher relative potassium conductance (t
K=0.6), lower intracellular Na+......
Na+-coupled glycine transport in reticulocyte vesicles of distinct sidedness: Stoichiometry and symmetry
The Journal of Membrane Biology - Tập 86 - Trang 37-44 - 1985
Sodium-coupled glycine transport has been studied using membrane vesicles of distinct sidedness, either inside-out or right side-out, prepared from sheep reticulocytes. The activity is chloride dependent and characterized by high and low apparent affinities for glycine (K′
m
≅0.5mm and >10mm) for both types of vesicles as well as intact cells. Transport is symmet......
pH- and voltage-dependent conductances in toad skin
The Journal of Membrane Biology - Tập 148 - Trang 1-11 - 1995
The present study focuses on two closely related topics on ion conductance in toad skins: (i) the interaction of apical protons with the apical voltage-dependent Cl−-activated channels of the mitochondriarich cells, and (ii) the description and characterization of a novel subject, a voltage-dependent H+-activated conductance. The Cl− conductance (G
Cl) is activated by tissue hyperpolarization (whi......
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