Role of passive potassium fluxes in cell volume regulation in cultured HeLa cells
Tóm tắt
Cultured HeLa cells behave as ideal osmometers when subjected to hyperosmolar media, and show no volume regulatory behavior. In hypoosmolar solutions, cell swelling is not as great as predicted, and this is due largely to a loss of intracellular KCl. In hyperosmolar solutions there is a stimulation of the ouabain-insensitive but loop diuretic-sensitive86Rb+ (K+) pathway. Analysis of the K+, Na+ and Cl− dependency of this K+ flux pathway demonstrates that the increase is principally due to an increase in its maximal velocity (V
max). The sensitivity of this pathway to diuretic inhibition is unchanged in hyperosmolar media. Diuretic-sensitive86Rb+ (K+) efflux stimulated by hypertonicity shows no marked dependence on external K+. The K+ loss observed in hypoosmolar media is distinct from the K+ transport pathway stimulated by hyperosmolar media on the basis of its sensitivity to furosemide and anion dependence.
Tài liệu tham khảo
Adragna, N.C., Tosteson, D.C. 1984. Effect of volume changes on ouabain-insensitive net outward cation movements in human red cells.J. Membrane Biol. 78:43–52
Aiton, J.F., Chipperfield, A.R., Lamb, J.F., Ogden, P., Simmons, N.L. 1981. Occurrence of passive furosemide-sensitive transmembrane transport in cultured cells.Biochim. Biophys. Acta 646:389–398
Aiton, J.F., Simmons, N.L. 1983. Effect of ouabain upon diuretic-sensitive K transport in cultured cells.Biochim. Biophys. Acta 734:279–289
Aiton, J.F., Simmons, N.L., Tivey, D.R. 1984. The effects of hypertonicity upon diuretic-sensitive K transport in cultured HeLa cells.J. Physiol. (London) 346:103P
Bakker-Grunwald, T. 1983. Potassium permeability and volume control in isolated rat hepatocytes.Biochim. Biophys. Acta 731:239–242
Blackstock, E.J., Ellory, J.C., Stewart, G.W. 1985. N-methyl-D-glucamine as a cation replacement for human redcell transport studies.J. Physiol. (London) 358:90P
Boardman, L., Huett, M., Lamb, J.F., Newton, J.P., Polson, J. 1974. Evidence for the genetic control of Na pump density in HeLa cells.J. Physiol. (London) 241:771–794
Brazy, P.C., Gunn, R.B. 1976. Furosemide inhibition of Cl transport in human red blood cells.J. Gen. Physiol. 68:583–599
Bui, A.H., Wiley, J.S. 1981. Cation fluxes and volume regulation by human lymphocytes.J. Cell. Physiol. 108:47–54
Cala, P.M. 1977. Volume regulation by flounder red blood cells in anisotonic media.J. Gen. Physiol. 69:537–552
Cala, P.M. 1983. Volume regulation by red blood cells.Mol. Physiol. 4:33–52
Chipperfield, A.R. 1984. Passive K influx into human red cells: Ion substitution experiments.Proc. Physiol. Soc., March meeting (in press)
Duhm, J., Gobel, B.O. 1984. Na and K transport and volume of rat erythrocytes under dietary K deficiency.Am. J. Physiol. 246:C20-C29
Dunham, P.G., Ellory, J.C. 1981. Passive potassium transport in LK sheep red cells: Dependence upon cell volume and chloride.J. Physiol. (London) 318:215–244
Ellory, J.C., Dunham, P.B., Logue, P.J., Stewart, G.W. 1982. Anion dependent cation transport in erythrocytes.Philos. Trans. R. Soc. London B 299:483–495
Geck, P., Heinz, E., Pfeiffer, B. 1981. Influence of high ceiling diuretics on ion fluxes and cell volume of Ehrlich ascites tumour cells.Scand. Audiol. Suppl.14:25–37
Geck, P., Pietrzyk, C., Burckhardt, B.C., Pfeiffer, B., Heinz, E. 1980. Electrically silent cotransport of Na, K, and Cl in Ehrlich cells.Biochim. Biophys. Acta 600:432–447
Grinstein, S., Clarke, C.A., Rothstein, A. 1982. Increased anion permeability during volume regulation in human lymphocytes.Philos. Trans. R. Soc. London B 299:509–518
Grinstein, S., Clarke, C.A., Rothstein, A. 1983. Activation of Na/H exchange in lymphocytes by osmotically induced volume changes and by cytoplasmic acidification.J. Gen. Physiol. 82:619–638
Grinstein, S., Clarke, C.A., Rothstein, A., Gelfand, E.W. 1983. Volume-induced anion conductance in human B lymphocytes is cation independent.Am. J. Physiol. 245:C160-C163
Haas, M., McManus, T.J. 1983. Bumetanide inhibits [Na+ K+2Cl] cotransport at a chloride site.Am. J. Physiol. 245:235–240
Haas, M., McManus, T.J. 1984. Effect of norepinephrine on swelling induced K transport in duck red cells; evidence against a volume regulatory decrease under physiological conditions.Fed. Proc. 43:1077 (abstr.)
Haas, M., Schmidt, W.F., McManus, T.J. 1982. Catecholamine stimulated ion transport in duck red cells. Gradient effects in electrically neutral [Na+K+2Cl] co-transport.J. Gen. Physiol. 80:125–147
Hoffman, E.K. 1982. Anion exchange and anion-cation cotransport systems in mammalian cells.Philos. Trans. R. Soc. London B 299:519–535
Hoffman, E.K., Sjøholm, C., Simonsen, L.O. 1983. Na+, Cl− cotransport in Ehrlich ascites tumor cells activated during volume regulation (regulatory volume increase).J. Membrane Biol. 76:269–280
House, R. 1973. Water relations of cells.In: Water Transport in Cells and Tissues. Monographs of the Physiological Society. Vol. 24, pp. 192–207. Edward Arnold, London
Kletzein, R.F., Pariza, M.W., Becker, J.E., Potter, V.R. 1975. A method using 3-O-methyl glucose and phloretin for the determination of intracellular water space of cells in monolayer culture.Anal. Biochem. 69:537–544
Knack, I., Rohm, K.H. 1981. Microcomputers in enzymology; a versatile BASIC program for analyzing kinetic data.Hoppe Seyler's Z. Physiol. Chem. 362:1119–1130
Kregenow, F.M. 1977. Transport in avian red cells.In: Membrane Transport in Red Cells. J.C. Ellory and V.L. Lew, editors. pp. 383–426. Academic, New York
Lauf, P.K. 1982. Evidence for chloride dependent potassium and water transport induced by hypotonic stress in erythrocytes of the marine teleost,Opsanus tau.J. Comp. Physiol. 146:9–16
Lauf, P.K. 1984. Thiol-dependent passive K/Cl transport in sheep red cells: IV. Furosemide inhibition as a function of external Rb+, Na+ and Cl−.J. Membrane Biol. 77:57–62
Lauf, P.K., Adragna, N.C., Garay, R.P. 1984. Activation by N-ethyl maleimide of a latent K+−Cl− flux in human red blood cells.Am. J. Physiol. 246:C385-C390
Lauf, P.K., Theg, B.E. 1980. A chloride-dependent K+ flux induced by N-ethylmaleimide in genetically low K+ sheep and goat erythrocytes.Biochem. Biophys. Res. Commun. 92:1422–1428
Macknight, A.D.C., Leaf, A. 1977. Regulation of cellular volume.Physiol. Rev. 57:510–573
McRoberts, J.A., Trong-Trang, C., Saier, M.H. 1983. Characterisation of low potassium-resistant mutants of the Madin-Darby canine kidney cell-line with defects in the NaCl/KCl symport.J. Biol. Chem. 258:12320–12326
Palfrey, H.C., Rao, M.C. 1983. Na/K/Cl cotransport and its regulation.J. Exp. Biol. 106:43–54
Reuss, L., Finn, A.L. 1975. Electrical properties of the cellular transepithelial pathway inNecturus gallbladder. I. Circuit analysis and steady-state effects of mucosal solution ionic substitutions.J. Membrane Biol. 25:115–139
Schmidt, W.F., McManus, T.J. 1977a. Ouabain-insensitive salt and water movements in duck red cells. I. Kinetics of cation transport under hypertonic conditions.J. Gen. Physiol. 70:59–79
Schmidt, W.F., McManus, T.J. 1977b. Ouabain-insensitive salt and water movements in duck red cells. II. Norepinephrine stimulation of sodium plus potassium cotransport.J. Gen. Physiol. 70:81–97
Schmidt, W.F., McManus, T.J. 1977c. Ouabain-insensitive salt and water movements in duck red cells. III. The role of Cl in the volume response.J. Gen. Physiol. 70:99–121
Simmons, N.L. 1984. Epithelial cell volume regulation in hypotonic fluids: Studies using a model tissue-culture system.Quart. J. Physiol. 69:83–95
Thornhill, W.B., Laris, P.C. 1984. KCl loss and cell shrinkage in the Erhlich ascites tumour cell induced by hypotonic media, 2-deoxyglucose and propananol.Biochim. Biophys. Acta 773:207–218
Ueberschar, S., Bakker-Grunwald, T. 1983. Bumetanide sensitive potassium transport and volume regulation in turkey erythrocytes.Biochim. Biophys. Acta 731:243–250