Biothermodynamic analysis of BSA adsorption to alum gel using isothermal titration calorimetrySpringer Science and Business Media LLC - Tập 12 - Trang 366-371 - 2007
Ki Hyung Kim, Eun Kyu Lee
In this study, we used ITC (isothermal titration calorimetry) to quantitatively investigate the impacts of temperature and protein concentration on adsorption behavior on a solid surface, using BSA (bovine serum albumin) as a model protein, and alum (aluminum hydroxide) gel as an adsorbent. The zeta potential measurement for alum gel (0.25 mV at pH 9.3) revealed that its surface charge was not str...... hiện toàn bộ
Rosin microparticles as drug carriers: Influence of various solvents on the formation of particles and sustained-release of indomethacinSpringer Science and Business Media LLC - Tập 9 - Trang 476-481 - 2004
Chang-Moon Lee, Seung Lim, Gwang-Yun Kim, Doman Kim, Dong-Woon Kim, Hyun-Chul Lee, Ki-Young Lee
The aim of this study was to formulate a sustained release system for indomethacin (IND) with rosin gum obtained from a pine tree. Rosin microparticles were prepared by a dispersion and dialysis method without the addition of surfactant. In order to investigate the influence of solvents on the formation of colloidal microparitcles, various solvents like ethanol, DMF, DMAc, and acetone were used. T...... hiện toàn bộ
Monitoring growth and lipid production of new isolated oleaginous yeast Cryptococcus aerius UIMC65 on glucose and xylose culturesSpringer Science and Business Media LLC - Tập 19 - Trang 468-477 - 2014
Hossein Ghanavati, Iraj Nahvi, Rasoul Roghanian
Process monitoring is one of the most important factors affecting production efficiency at industrial scale bioprocesses. In the present work, Flow-cytometric analysis has been employed to monitor and determine neutral lipid cell droplets, granularity and size of the cells of the new oleaginous yeast, Cryptococcus aerius UIMC65. It has been shown that, differences of fluorescent intensity as well ...... hiện toàn bộ
Virus-like Particles as Antiviral Vaccine: Mechanism, Design, and ApplicationSpringer Science and Business Media LLC - Tập 28 - Trang 1-16 - 2023
Lei Zhang, Wen Xu, Xi Ma, XiaoJing Sun, JinBo Fan, Yang Wang
Virus-like particles (VLPs) are viral structural protein that are noninfectious as they do not contain viral genetic materials. They are safe and effective immune stimulators and play important roles in vaccine development because of their intrinsic immunogenicity to induce cellular and humoral immune responses. In the design of antiviral vaccine, VLPs based vaccines are appealing multifunctional ...... hiện toàn bộ
Improvement of pristinamycin production by genome shuffling and medium optimization for Streptomyces pristinaespiralisSpringer Science and Business Media LLC - Tập 14 - Trang 175-179 - 2009
Bo Xu, Zhihua Jin, Qingchao Jin, Ninghui Li, Peilin Cen
To isolate an improved pristinamycin producing strain of Streptomyces pristinaespiralis, the technique of Genome shuffling was used which resulted in a high-yield recombinant G 3-56 strain. Strain G 3-56 yielded 322 ± 17 mg/L of pristinamycin which was 11.4-fold higher than that of the initial strain and 3.7-fold higher than strain UN-78 which previously had the highest yield of pristinamycin. The...... hiện toàn bộ
Hair multiplication with dermal papilla like tissue containing human dermal papilla cellsSpringer Science and Business Media LLC - Tập 19 - Trang 394-403 - 2014
Bo-Young Yoo, Youn-Ho Shin, Hee-Hoon Yoon, Young-Kwon Seo, Jung-Keug Park
Alopecia is not a critical disease; however it is a disease that can affect the quality of life. Many remedies have been developed to cure alopecia, but only two have been approved by the FDA. Due to the steadily increasing number of young alopecia patients, the need for new therapies for curing alopecia is very high. Recent studies on cell therapy have reported using technique to treat various di...... hiện toàn bộ
In situ carbon supplementation in large-scale cultivations of Spirulina platensis in open raceway pondSpringer Science and Business Media LLC - Tập 17 - Trang 93-99 - 2012
Yilu Bao, Ming Liu, Xia Wu, Wei Cong, Zhengxiang Ning
The objective of this study was to estimate the CO2 absorptivity provided by an in situ carbon supply system using a photosynthetic culture of the cyanobacterium Spirulina platensis in an open raceway pond. The effects of initial total carbon concentrations (ranging from 0 to 0.1 mol/L), suspension depths (ranging from 5 to 20 cm) and pH values (ranging from 8.9 to 11.0) on the CO2 absorptivity we...... hiện toàn bộ