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Efficient production of diltiazem chiral intermediate using immobilized lipase from Serratia marcescens
Springer Science and Business Media LLC - Tập 15 - Trang 199-207 - 2010
The lipase from Serratia marcescence ECU1010 (Sml) was capable of enantioselectively catalyzing the synthesis of many chiral drug precursors. This paper investigated the immobilization of Sml on appropriate supporting materials and its performance in bioreactor. Chitosan, Celite 545, and DEAE-cellulose were found to be the ideal supports among 8 carriers tested with respect to enzyme load and acti...... hiện toàn bộ
Generation of a naïve/synthetic antibody specific to botulinum neurotoxin via motif-grafting
Springer Science and Business Media LLC - - 2007
Use of antioxidants to extend the storage of lyophilized cell-free synthesis system
Springer Science and Business Media LLC -
Surface display of lipolytic enzyme, Lipase A and Lipase B of Bacillus subtilis on the Bacillus subtilis spore
Springer Science and Business Media LLC - Tập 22 - Trang 462-468 - 2017
For the enhancement of lipase stability in organic solvent containing reaction, live immobilization method, using Bacillus subtilis spore as a display vehicle was attempted. Bacillus subtilis coat protein cotE was used as an anchoring motif for the display of lipA and lipB of Bacillus subtilis. Using this motif, lipolytic enzyme Lipase A and Lipase B were functionally displayed on the surface of B...... hiện toàn bộ
Effects of different mineral supplements on fertilization of phenol-contaminated soils by Corynebacterium glutamicum
Springer Science and Business Media LLC - Tập 19 - Trang 276-281 - 2014
This study focused on the effects of different mineral supplements on the ability of Corynebacterium glutamicum to degrade phenol in contaminated soil and convert the phenol into useful amino acids. Three types of minerals including FeSO4, MgSO4, and MnSO4 were added at several concentrations to C. glutamicum culture media containing 1% yeast extract prior to treating the soil samples with 4.24 mM...... hiện toàn bộ
Xylanase enzyme from novel strain and its immobilization onto metal organic framework MOF for fruit juice clarification
Springer Science and Business Media LLC - Tập 29 Số 1 - Trang 197-210 - 2024
Response surface methodology analysis of anaerobic syntrophic degradation of volatile fatty acids in an upflow anaerobic sludge bed reactor inoculated with enriched cultures
Springer Science and Business Media LLC - Tập 17 - Trang 133-144 - 2012
Anaerobic oxidation of volatile fatty acids (VFAs) as the key intermediates is restricted thermodynamically. Presently, enriched acetogenic and methanogenic cultures were used for syntrophic anaerobic digestion of VFAs in an upflow anaerobic sludge bed reactor fed with acetic, propionic, and butyric acids at maximum concentrations of 5.0, 3.0, and 4.0 g/L, respectively. Interactive effects of prop...... hiện toàn bộ
In vitro Anti-cancer Efficacy and Cellular Interaction of Cubic Phases Containing Cinnamic Acid, Poly(ethyleneimine), and Doxorubicin
Springer Science and Business Media LLC - Tập 25 - Trang 235-245 - 2020
Folate-decorated monoolein (MO) cubosomes containing poly(ethyleneimine) (PEI), cinnamic acid (CA), and doxorubicin (DOX) were prepared to deliver the anticancer drug specifically to cancer cells (KB cells) and boost the anti-cancer efficacy without causing an acute toxicity. Hydrophobically modified (Hm) gelatin (Gel)-folate (FA) conjugate (HmGel-FA) was prepared through amidation reaction among ...... hiện toàn bộ
Cải thiện nồng độ iso-migrastatin trong chủng Streptomyces lividans SB11002 được tinh chỉnh thông qua tối ưu hóa điều kiện lên men Dịch bởi AI
Springer Science and Business Media LLC - Tập 15 - Trang 664-669 - 2010
Sự sản xuất heterologous iso-migrastatin (iso-MGS) đã được chứng minh thành công trong một chủng S. lividans SB11002 được thiết kế, được phát triển từ S. lividans K4-114, sau khi đưa vào pBS11001, chứa toàn bộ cụm gen sinh tổng hợp mgs. Tuy nhiên, dưới các điều kiện lên men tương tự, nồng độ iso-MGS trong chủng đã được thiết kế thấp hơn đáng kể so với ở nhà sản xuất bản địa — Streptomyces platensi...... hiện toàn bộ
#iso-migrastatin #Streptomyces lividans #tối ưu hóa môi trường lên men #sinh tổng hợp
Treatment of growth plate injury with microencapsulated chondrocytes
Springer Science and Business Media LLC - Tập 18 - Trang 655-662 - 2013
Tissue-engineered cartilage has provided a promising method in the treatment of physeal growth arrest. This study was designed to investigate transplantation of microencapsulated allogeneic chondrocytes to treat the injured growth plate. Allogeneic chondrocytes were encapsulated within alginate-polylysinealginate semipermeable membranes. Microencapsulated chondrocytes co-cultured with Bone Mesench...... hiện toàn bộ
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