Biothermodynamic analysis of BSA adsorption to alum gel using isothermal titration calorimetrySpringer Science and Business Media LLC - Tập 12 - Trang 366-371 - 2007
Ki Hyung Kim, Eun Kyu Lee
In this study, we used ITC (isothermal titration calorimetry) to quantitatively investigate the impacts of temperature and protein concentration on adsorption behavior on a solid surface, using BSA (bovine serum albumin) as a model protein, and alum (aluminum hydroxide) gel as an adsorbent. The zeta potential measurement for alum gel (0.25 mV at pH 9.3) revealed that its surface charge was not str...... hiện toàn bộ
Improvement of Tol2 Transposon System Enabling Efficient Protein Production in CHO CellsSpringer Science and Business Media LLC - Tập 26 - Trang 767-775 - 2021
Su Young Hwang, Yun Haeng Lee, Myeong Uk Kuk, Jae Won Kim, Sekyung Oh, Joon Tae Park
Establishment of mammalian cell lines with high protein productivity is an important object in the field of biopharmaceutics. Toward this end, Tol2 transposon-based expression systems have been developed as effective means to facilitate protein productivity. Here, we proposed novel strategies to improve conventional Tol2 transposon systems. The use of Tol2 transposase mRNA as a helper vector impro...... hiện toàn bộ
Rosin microparticles as drug carriers: Influence of various solvents on the formation of particles and sustained-release of indomethacinSpringer Science and Business Media LLC - Tập 9 - Trang 476-481 - 2004
Chang-Moon Lee, Seung Lim, Gwang-Yun Kim, Doman Kim, Dong-Woon Kim, Hyun-Chul Lee, Ki-Young Lee
The aim of this study was to formulate a sustained release system for indomethacin (IND) with rosin gum obtained from a pine tree. Rosin microparticles were prepared by a dispersion and dialysis method without the addition of surfactant. In order to investigate the influence of solvents on the formation of colloidal microparitcles, various solvents like ethanol, DMF, DMAc, and acetone were used. T...... hiện toàn bộ
Monitoring growth and lipid production of new isolated oleaginous yeast Cryptococcus aerius UIMC65 on glucose and xylose culturesSpringer Science and Business Media LLC - Tập 19 - Trang 468-477 - 2014
Hossein Ghanavati, Iraj Nahvi, Rasoul Roghanian
Process monitoring is one of the most important factors affecting production efficiency at industrial scale bioprocesses. In the present work, Flow-cytometric analysis has been employed to monitor and determine neutral lipid cell droplets, granularity and size of the cells of the new oleaginous yeast, Cryptococcus aerius UIMC65. It has been shown that, differences of fluorescent intensity as well ...... hiện toàn bộ