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Biothermodynamic analysis of BSA adsorption to alum gel using isothermal titration calorimetry
Springer Science and Business Media LLC - Tập 12 - Trang 366-371 - 2007
Ki Hyung Kim, Eun Kyu Lee
In this study, we used ITC (isothermal titration calorimetry) to quantitatively investigate the impacts of temperature and protein concentration on adsorption behavior on a solid surface, using BSA (bovine serum albumin) as a model protein, and alum (aluminum hydroxide) gel as an adsorbent. The zeta potential measurement for alum gel (0.25 mV at pH 9.3) revealed that its surface charge was not str...... hiện toàn bộ
Virus-like Particles as Antiviral Vaccine: Mechanism, Design, and Application
Springer Science and Business Media LLC - Tập 28 - Trang 1-16 - 2023
Lei Zhang, Wen Xu, Xi Ma, XiaoJing Sun, JinBo Fan, Yang Wang
Virus-like particles (VLPs) are viral structural protein that are noninfectious as they do not contain viral genetic materials. They are safe and effective immune stimulators and play important roles in vaccine development because of their intrinsic immunogenicity to induce cellular and humoral immune responses. In the design of antiviral vaccine, VLPs based vaccines are appealing multifunctional ...... hiện toàn bộ
Improvement of pristinamycin production by genome shuffling and medium optimization for Streptomyces pristinaespiralis
Springer Science and Business Media LLC - Tập 14 - Trang 175-179 - 2009
Bo Xu, Zhihua Jin, Qingchao Jin, Ninghui Li, Peilin Cen
To isolate an improved pristinamycin producing strain of Streptomyces pristinaespiralis, the technique of Genome shuffling was used which resulted in a high-yield recombinant G 3-56 strain. Strain G 3-56 yielded 322 ± 17 mg/L of pristinamycin which was 11.4-fold higher than that of the initial strain and 3.7-fold higher than strain UN-78 which previously had the highest yield of pristinamycin. The...... hiện toàn bộ
In situ carbon supplementation in large-scale cultivations of Spirulina platensis in open raceway pond
Springer Science and Business Media LLC - Tập 17 - Trang 93-99 - 2012
Yilu Bao, Ming Liu, Xia Wu, Wei Cong, Zhengxiang Ning
The objective of this study was to estimate the CO2 absorptivity provided by an in situ carbon supply system using a photosynthetic culture of the cyanobacterium Spirulina platensis in an open raceway pond. The effects of initial total carbon concentrations (ranging from 0 to 0.1 mol/L), suspension depths (ranging from 5 to 20 cm) and pH values (ranging from 8.9 to 11.0) on the CO2 absorptivity we...... hiện toàn bộ
Cultural characteristics and extraction of the fungal pigment phleichrome from the phytopathogenic fungusCladosporium phlei
Springer Science and Business Media LLC - - 2007
Joong-Keun Lee, Beom-Tae Kim, Jung-Ae Kim, Hea-Jong Chung, Seung-Moon Park, Moon-Sik Yang, Ki-Jun Hwang, Dae-Hyuk Kim
The cultural characteristics of the fungusCladosporium phlei were assessed in order to develop an improved method for the production of the fungal pigment, phleichrome, which is an intermediate in the production of a photodynamic therapeutic agent. The growth ofC. phlei, as measured by the hyphal growth rate and increase in biomass, varies significantly depending on the culture media utilized (V8 ...... hiện toàn bộ
Decreased Growth and Antibiotic Production in Streptomyces coelicolor A3(2) by Deletion of a Highly Conserved DeoR Family Regulator, SCO1463
Springer Science and Business Media LLC - Tập 24 - Trang 613-621 - 2019
Jong-Min Jeon, Tae-Rim Choi, Bo-Rahm Lee, Joo-Hyun Seo, Hun-Suk Song, Hye-Rim Jung, Soo-Yeon Yang, Jun Young Park, Eun-Jung Kim, Byung-Gee Kim, Yung-Hun Yang
Streptomyces spp. have been isolated from different environmental niches and are known to exhibit diversity in secondary metabolism. They have complex regulation system to control secondary metabolism, however it is difficult to prioritize the importance of specific regulator among the thousands of global or cluster-situated genes that may regulate secondary metabolism in different Streptomyces st...... hiện toàn bộ
Kinetic Modeling of cometabolic degradation of ethanethiol and phenol by Ralstonia eutropha
Springer Science and Business Media LLC - Tập 19 - Trang 239-249 - 2014
Mahsa Sedighi, Farzaneh Vahabzadeh
Cometabolism, as a complex phenomenon in microbial world, is a special mechanism for transformation of many compounds of environmental and toxicological significance. Several models have been proposed to describe the cometabolic transformations of non-growth substrates in the absence or presence of growth substrates. In this study, a model was proposed to simulate the degradation kinetics of pheno...... hiện toàn bộ
The role of high-throughput transcriptome analysis in metabolic engineering
Springer Science and Business Media LLC - Tập 10 - Trang 385-399 - 2005
Michael C. Jewett, Ana Paula Oliveira, Kiran Raosaheb Patil, Jens Nielsen
The phenotypic response of a cell results from a well orchestrated web of complex interactions which propagate from the genetic architecture through the metabolic flux network. To rationally design cell factories which carry out specific functional objectives by controlling this hierarchical system is a challenge. Transcriptome analysis, the most mature high-throughput measurement technology, has ...... hiện toàn bộ
Enhanced production of fructose palmitate by lipase-catalyzed esterification in ionic liquids
Springer Science and Business Media LLC - Tập 15 Số 1 - Trang 126-130 - 2010
Sung Ho Ha, Nguyễn Minh Hiệp, Yoon‐Mo Koo
Unusual metabolism of 3,6-anhydro-L-galactose in Vibrio sp. EJY3 and in E. coli containing two Vibrio sp. EJY3 genes
Springer Science and Business Media LLC - - 2015
Sun Bok Lee
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