Toward a better analysis of secreted proteins: the example of the myeloid cells secretomeProteomics - Tập 7 Số 11 - Trang 1757-1770 - 2007
Mireille Chevallet, Hélène Diemer, Alain Van Dorssealer, Christian Villiers, Thierry Rabilloud
AbstractThe analysis of secreted proteins represents a challenge for current proteomics techniques. Proteins are usually secreted at low concentrations in the culture media, which makes their recovery difficult. In addition, culture media are rich in salts and other compounds interfering with most proteomics techniques, which makes selective precipitation of protei...... hiện toàn bộ
ATR inhibition rewires cellular signaling networks induced by replication stressProteomics - Tập 16 Số 3 - Trang 402-416 - 2016
Sebastian Wagner, Hannah Oehler, Andrea Voigt, Denis Dalic, Anja Freiwald, Hubert Serve, Petra Beli
The slowing down or stalling of replication forks is commonly known as replication stress and arises from multiple causes such as DNA lesions, nucleotide depletion, RNA‐DNA hybrids, and oncogene activation. The ataxia telangiectasia and Rad3‐related kinase (ATR) plays an essential role in the cellular response to replication stress and inhibition of ATR has emerged as therapeutic strategy ...... hiện toàn bộ
A posteriori quality control for the curation and reuse of public proteomics dataProteomics - Tập 11 Số 11 - Trang 2182-2194 - 2011
Joseph Foster, Sven Degroeve, Laurent Gatto, Matthieu Visser, Rui Wang, Johannes Griss, Rolf Apweiler, Lennart Martens
AbstractProteomics is a rapidly expanding field encompassing a multitude of complex techniques and data types. To date much effort has been devoted to achieving the highest possible coverage of proteomes with the aim to inform future developments in basic biology as well as in clinical settings. As a result, growing amounts of data have been deposited in publicly a...... hiện toàn bộ
XTandem Parser: An open‐source library to parse and analyse X!Tandem MS/MS search resultsProteomics - Tập 10 Số 7 - Trang 1522-1524 - 2010
Thilo Muth, Marc Vaudel, Harald Barsnes, Lennart Martens, Albert Sickmann
AbstractIdentification of proteins by MS plays an important role in proteomics. A crucial step concerns the identification of peptides from MS/MS spectra. The X!Tandem Project (http://www.thegpm.org/tandem) supplies an open‐source search engine for this purpose. In this study, we present an open‐source Java library called XTandem Parser that parses X!Tandem XML result files into an easily accessible and fully functional object model ( hiện toàn bộ
Plasma membrane proteome in Arabidopsis and riceProteomics - Tập 8 Số 19 - Trang 4137-4145 - 2008
Setsuko Komatsu
AbstractPlant cells contain many membrane systems that are specially adapted to perform particular functions. In plant cells, the processing of signals that are involved in responses to biotic and abiotic stressors occurs in the plasma membrane. Therefore, characterization of the plasma membrane proteome can provide new insights into the functions of various plant ...... hiện toàn bộ
Proteome analysis of human colon cancer by two‐dimensional difference gel electrophoresis and mass spectrometryProteomics - Tập 4 Số 3 - Trang 793-811 - 2004
David B. Friedman, Salisha Hill, Jeffrey W. Keller, Nipun B. Merchant, Shawn Levy, Robert J. Coffey, Richard M. Caprioli
AbstractTwo‐dimensional difference gel electrophoresis (2‐D DIGE) coupled with mass spectrometry (MS) was used to investigate tumor‐specific changes in the proteome of human colorectal cancers and adjacent normal mucosa. For each of six patients with different stages of colon cancer, Cy5‐labeled proteins isolated from tumor tissue were combined with Cy3‐labeled pro...... hiện toàn bộ