Sylvie Luche1, Véronique Santoni2, Thierry Rabilloud1
1BECP - Bioénergétique Cellulaire et Pathologique (CEA-Grenoble 17 rue des martyrs F-38054 Grenoble cedex9 - France)
2BPMP - Biochimie et Physiologie Moléculaire des Plantes (Bât. 7 - 2 place Pierre Viala - 34060 Montpellier cedex 2 - France)
Tóm tắt
AbstractThe solubilizing power of various nonionic and zwitterionic detergents as membrane protein solubilizers for two‐dimensional electrophoresis was investigated. Human red blood cell ghosts and Arabidopsis thaliana leaf membrane proteins were used as model systems. Efficient detergents could be found in each class, i.e. with oligooxyethylene, sugar or sulfobetaine polar heads. Among the commercially available nonionic detergents, dodecyl maltoside and decaethylene glycol mono hexadecyl ether proved most efficient. They complement the more classical sulfobetaine detergents to widen the scope of useful detergents for the solubilization of membrane proteins in proteomics.
