Pectin from Three Vietnamese Seagrasses: Isolation, Characterization and Antioxidant ActivityProcesses - Tập 11 Số 4 - Trang 1054 - 2023
Thinh, Pham Duc, Hang, Cao Thi Thuy, Trung, Dinh Thanh, Nguyen, Thanh-Danh
This study focused on the isolation and structural characterization of pectin from three distinct species of Vietnamese seagrass including Enhalus acoroides, Thalassia hemprichii, and Halophila ovalis. The pectin yield obtained from Enhalus acoroides was the highest, corresponding to 24.15%, followed by those from Thalassia hemprichii (20.04%) and Halophila ovalis (19.14%). The physicochemical properties of pectin including total carbohydrate content, anhydrouronic acid (AUA) content, equivalent weight (EW), methoxyl content (MeO), and degree of esterification (DE) were determined using various analysis techniques. The pectin obtained from all three species were found to be low-methyl-esterified pectin, with the MeO content and DE for E. acoroides, T. hemprichii, and H. ovalis being 6.15% and 27.18%, 3.26% and 43.31%, and 4.65% and 33.25%, respectively. The average molecular weight (MW) of pectin was analyzed by size-exclusion chromatography. Pectin from T. hemprichii had the highest MW of 173.01 kDa, followed by pectin from E. acoroides, with a MW of 127.32 kDa, and that from H. ovalis, with a MW of 56.06 kDa. Furthermore, the pectins from all three seagrass species exhibited high antioxidant activity and might be promising as antioxidants.
Experimental Study of a Gas-Liquid-Solid Three-Phase Flow in an Aeration Tank Driven by an Inverted Umbrella AeratorProcesses - Tập 10 Số 7 - Trang 1278
Siyuan Hu, Liang Dong, Runan Hua, Jinnan Guo, Houlin Liu, Cui Dai
The three-phase flow in a aeration tank driven by an inverted umbrella aerator is relatively complex, including the processes of the hydraulic jump, air entrainment, and sludge particle sedimentation. A three-phase flow test bench for an inverted umbrella aerator is established for studying its influence on aeration performance. The experiment mainly studies the changed law of aeration performance under different immersion depths or sludge concentrations and measures the flow rate and sludge concentrations in the aeration tank in different working conditions. The results are as follows. (1) The total oxygen transfer coefficient, standard oxygenation capacity, and standard power efficiency increase with the increase in rotational speed. The total oxygen transfer coefficient and standard-charge oxygen capacity first increase and then decrease with the decrease in immersion depth, reaching a maximum at −20 mm immersion depth. The standard dynamic efficiency has a similar trend and reaches a maximum at −8 mm immersion depth. (2) In the aeration tank, the flow velocity near the impeller is faster and has greater turbulence. The shallow water is more profoundly affected by the impeller compared with the deeper water. (3) The shallow-water sludge varies greatly, and the deep-water sludge is distributed uniformly when the inverted umbrella aerator works stably.
Recovery of Phenolic Compounds and Antioxidants from Coffee Pulp (Coffea canephora) Waste Using Ultrasound and Microwave-Assisted ExtractionProcesses - Tập 10 Số 5 - Trang 1011 - 2022
Tran, Thy Minh Kieu, Akanbi, Taiwo O., Kirkman, Timothy, Nguyen, Minh H., Vuong, Quan Van
Coffee pulp is a by-product generated from coffee bean production. This waste is a potential source of bioactive compounds, which can be recovered for use as an ingredient for many products. However, this by-product is typically dumped in landfills or made into compost. Ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) were employed to recover bioactive compounds from coffee pulp waste. Results showed that time and instrument power significantly affected the recovery yield in both UAE and MAE. The temperature was also a significant factor in UAE. The optimal MAE conditions were a radiation time of 70 min, a power of 700 W, and a 50% (v/v) ethanol solvent to sample ratio of 100:5 (mL/g), approximately 47 mg of phenolic compounds, 36 mg of flavonoid, 8 mg of chlorogenic acid, and 6 mg of caffeine could be recovered from 1 g of the material. The optimal UAE condition were an ultrasonic time of 35 min, a temperature of 60 °C, and a power of 250 W; however, bioactive compounds and antioxidant capacity constituted around one half of MAE. Therefore, MAE is recommended as the extraction technique for the bioactive compound and antioxidant recovery from the coffee pulp.
Ultrasound-Assisted Extraction of GAC Peel: An Optimization of Extraction Conditions for Recovering Carotenoids and Antioxidant CapacityProcesses - Tập 8 Số 1 - Trang 8 - 2020
Chuyen, Hoang V., Roach, Paul D., Golding, John B., Parks, Sophie E., Nguyen, Minh H.
The peel of Gac fruit (Momordica cochinchinensis Spreng.), which is considered as waste of Gac processing, has been found to possess high levels of carotenoids and other antioxidants. This study aimed at determining the optimal conditions of an ultrasound-assisted extraction for recovering carotenoids and antioxidant capacity from Gac peel. A response surface methodology using the Box–Behnken design was employed to investigate the impact of extraction time, temperature and ultrasonic power on the recovery of total carotenoid and antioxidant capacity. The results showed that an extraction time of 76 min, temperature of 50 °C and ultrasonic power of 250 W were the optimal conditions for the extraction. The experimental carotenoid yield and antioxidant capacity obtained under the optimal extraction conditions were validated as 269 mg/100 g DW (dry weight) and 822 µM TE (Trolox equivalent)/100 g DW, respectively. These values were not significantly different from the values predicted by the models. The HPLC analysis for carotenoid composition showed that β-carotene, lycopene and lutein were the principal carotenoids of the extract, which constitute 86% of the total carotenoid content. Based on the obtained results, the ultrasound-assisted extraction using ethyl acetate under the above optimal conditions is suggested for the simultaneous recovery of carotenoids and antioxidant capacity from Gac peel.
Proprotein Convertase Subtilisin/Kexin Type 9 Gene Variants in Familial Hypercholesterolemia: A Systematic Review and Meta-AnalysisProcesses - Tập 9 Số 2 - Trang 283 - 2021
Pham, Nang Hoang, Truong, Phuong Kim, Lao, Thuan Duc, Le, Thuy Ai Huyen
Proprotein Convertase Subtilisin Kexin type 9 (PCSK9), comprises 12 exons, encoded for an enzyme which plays a critical role in the regulation of circulating low density lipoprotein. The gain-of-function (GOF) mutations aggravate the degradation of LDL receptors, resulting in familial hypercholesterolemia (FH), while loss-of-function (LOF) mutations lead to higher levels of the LDL receptors, lower the levels of LDL cholesterol, and preventing from cardiovascular diseases. It is noted that, previous publications related to the mutations of PCSK9 were not always unification. Therefore, this study aims to present the spectrum and distribution of PCSK9 gene mutations by a meta-analysis. A systematic literature analysis was conducted based on previous studies published by using different keywords. The weighted average frequency of PCSK9 mutation was calculated and accessed by MedCalc®. A total of 32 cohort studies, that included 19,725 familial hypercholesterolemia blood samples, were enrolled in the current study. The analysis results indicated that, based on the random-effect model, the weighted prevalence of PCSK9 mutation was 5.67% (95%CI = 3.68–8.05, p < 0.0001). The prevalence of PCSK9 GOF mutations was 3.57% (95%CI = 1.76–5.97, p < 0.0001) and PCSK9 LOF mutations was 6.05% (95%CI = 3.35–9.47, p < 0.0001). Additionally, the first and the second exon were identified as the hot spot of mutation occurred in PCSK9. Both GOF and LOF mutations have a higher proportion in Asia and Africa compared with other regions. The GOF PCSK9 p.(Glu32Lys) and LOF PCSK9 p.(Leu21dup/tri) were dominant in the Asia region with the proportion as 6.58% (95%CI = 5.77–7.47, p = 0.62) and 16.20% (95%CI = 6.91–28.44, p = 0.0022), respectively. This systematic analysis provided scientific evidence to suggest the mutation of PCSK9 was related to the metabolism of lipoprotein and atherosclerotic cardiovascular disease.
Food Waste Composting and Microbial Community Structure ProfilingProcesses - Tập 8 Số 6 - Trang 723
Kishneth Palaniveloo, Muhammad Azri Amran, Nur Azeyanti Norhashim, Nuradilla Mohamad‐Fauzi, Fang Peng-Hui, Low Hui-Wen, Yap Kai-Lin, Looi Jiale, Melissa Goh Chian-Yee, Lai Jing-Yi, Baskaran Gunasekaran, Shariza Abdul Razak
Over the last decade, food waste has been one of the major issues globally as it brings a negative impact on the environment and health. Rotting discharges methane, causing greenhouse effect and adverse health effects due to pathogenic microorganisms or toxic leachates that reach agricultural land and water system. As a solution, composting is implemented to manage and reduce food waste in line with global sustainable development goals (SDGs). This review compiles input on the types of organic composting, its characteristics, physico-chemical properties involved, role of microbes and tools available in determining the microbial community structure. Composting types: vermi-composting, windrow composting, aerated static pile composting and in-vessel composting are discussed. The diversity of microorganisms in each of the three stages in composting is highlighted and the techniques used to determine the microbial community structure during composting such as biochemical identification, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), terminal restriction fragment length polymorphism (T-RFLP) and single strand-conformation polymorphism (SSCP), microarray analysis and next-generation sequencing (NGS) are discussed. Overall, a good compost, not only reduces waste issues, but also contributes substantially to the economic and social sectors of a nation.
A DEA Approach for Assessing the Energy, Environmental and Economic Performance of Top 20 Industrial CountriesProcesses - Tập 7 Số 12 - Trang 902
Wasim Iqbal, Ali Altalbe, Arooj Fatima, Amjad Ali, Yumei Hou
Due to growing concerns of global warming, reducing carbon emissions has become one of the major tasks for developing countries to meet the national demand for energy policies. The objective of this study is to measure the energy consumption, carbon emission and economic-environmental efficiency in terms of the environmental performance of the top 20 industrial countries by employing a data envelopment analysis (DEA) model from 2013 to 2017. This study used the trilemma of energy efficiency, CO2 emission efficiency, and environmental efficiency, and also the contribution included the quantitative analysis of 20 industrial countries The results show that the energy efficiency of Australia, China, Japan, Saudi Arabia, and Poland are the best performing countries, whereas Mexico, Indonesia, Russia, and Brazil are identified as least efficient among all 20 countries. Furthermore, Russia’s energy intensity has a maximum score while Poland has a minimum score. Additionally, in the case of CO2 emission efficiency, Brazil, France, and Saudi Arabia are considered as efficient while nine country’s scores were less than 0.5. The results show that most countries exhibit higher performance in economic efficiency than environmental efficiency. The study provides valuable information for energy policy-makers.
Measuring Energy Efficiency and Environmental Performance: A Case of South AsiaProcesses - Tập 7 Số 6 - Trang 325
Yumei Hou, Wasim Iqbal, Ghulam Muhammad Shaikh, Nadeem Iqbal, Yasir Ahmed Solangi, Arooj Fatima
When assessing energy efficiency, most studies have frequently ignored environmental aspects even though the concept has been widely used in the past. This study evaluates the energy efficiency and environmental performance of South Asia by using DEA (data envelopment analysis) like mathematical composite indicator. We construct a comprehensive set of indicators, including an energy self-sufficiency ratio, energy production over consumption ratio, energy imports, diversification index of energy imports, energy reserve ratio, GDP productivity, energy intensity, per capita energy consumption index, carbon emission index, carbon emission index per unit of energy consumption and share of renewable energy in order to develop an energy efficiency and environmental performance index. Unlike other studies, this study first examines each indicator and then estimates a combined score for each country. The results reveal that Bhutan as a more secure country and Pakistan showed a decreasing trend, while Sri Lanka and India performed satisfactorily. Remarkably, Bangladesh, Nepal and Afghanistan showed a decreasing trend. This study proposes a policy that increases the cross-border trade of renewable energy for long term energy efficiency and environmental performance.
Assessment of Wind Energy Potential for the Production of Renewable Hydrogen in Sindh Province of PakistanProcesses - Tập 7 Số 4 - Trang 196
Wasim Iqbal, Yumei Hou, Qaiser Abbas, Muhammad Hafeez, Muhammad Mohsin, Arooj Fatima, Maqsood Ali jamali, Mehwish Jamali, Afroze Siyal, Sohail M. Noman
In this study, we developed a new hybrid mathematical model that combines wind-speed range with the log law to derive the wind energy potential for wind-generated hydrogen production in Pakistan. In addition, we electrolyzed wind-generated power in order to assess the generation capacity of wind-generated renewable hydrogen. The advantage of the Weibull model is that it more accurately reflects power generation potential (i.e., the capacity factor). When applied to selected sites, we have found commercially viable hydrogen production capacity in all locations. All sites considered had the potential to produce an excess amount of wind-generated renewable hydrogen. If the total national capacity of wind-generated was used, Pakistan could conceivably produce 51,917,000.39 kg per day of renewable hydrogen. Based on our results, we suggest that cars and other forms of transport could be fueled with hydrogen to conserve oil and gas resources, which can reduce the energy shortfall and contribute to the fight against climate change and global warming. Also, hydrogen could be used to supplement urban energy needs (e.g., for Sindh province Pakistan), again reducing energy shortage effects and supporting green city programs.
Production, Optimization, and Partial Purification of Alkali-Thermotolerant Proteases from Newly Isolated Bacillus subtilis S1 and Bacillus amyloliquefaciens KSM12Processes - Tập 10 Số 6 - Trang 1050
Sidra Hashmi, Sajid Iqbal, Iftikhar Ahmed, Hussnain Ahmed Janjua
Proteases that can remain active under extreme conditions such as high temperature, pH, and salt concentration are widely applicable in the commercial sector. The majority of the proteases are rendered useless under harsh conditions in industries. Therefore, there is a need to search for new proteases that can tolerate and function in harsh conditions, thus improving their commercial value. In this study, 142 bacterial isolates were isolated from diverse alkaline soil habitats. The two highest protease-producing bacterial isolates were identified as Bacillus subtilis S1 and Bacillus amyloliquefaciens KSM12, respectively, based on 16S rRNA sequencing. Optimal protease production was detected at pH 8, 37 °C, 48 h, 5% (w/v) NaCl for Bacillus subtilis S1 (99.8 U/mL) and pH 9, 37 °C, 72 h, 10% (w/v) NaCl for Bacillus amyloliquefaciens KSM12 (94.6 U/mL). The molecular weight of these partially purified proteases was then assessed on SDS-PAGE (17 kDa for Bacillus subtilis S1 and 65 kDa for Bacillus amyloliquefaciens KSM12), respectively. The maximum protease activity for Bacillus subtilis S1 was detected at pH 8, 40 °C, and for Bacillus amyloliquefaciens KSM12 at pH 9, 60 °C. These results suggest that the proteases secreted by Bacillus subtilis S1 and Bacillus amyloliquefaciens KSM12 are suitable for industries working in a highly alkaline environment.