Diffusive reaction dynamics on invariant free energy profilesProceedings of the National Academy of Sciences of the United States of America - Tập 105 Số 37 - Trang 13841-13846 - 2008
Sergei V. Krivov, Martin Karplus
A fundamental problem in the analysis of protein folding and other complex reactions in which the entropy plays an important role is the determination of the activation free energy from experimental measurements or computer simulations. This article shows how to combine minimum-cut-based free energy profiles (
F
C...... hiện toàn bộ
Genomewide SNP variation reveals relationships among landraces and modern varieties of riceProceedings of the National Academy of Sciences of the United States of America - Tập 106 Số 30 - Trang 12273-12278 - 2009
Millicent D. Alexandrov Sanciangco, Kevin L. Childs, Regina Bohnert, Rebecca M. Davidson, Keyan Zhao, Victor Jun Ulat, Georg Zeller, Richard M. Clark, Douglas Hoen, Thomas E. Bureau, Renee Stokowski, Dennis G. Ballinger, Kelly A. Frazer, David R. Cox, Badri Padhukasahasram, Carlos D. Bustamante, Detlef Weigel, D. J. Mackill, Richard Bruskiewich, Gunnar Rätsch, C. Robin Buell, Hei Leung, Jan E. Leach
Rice, the primary source of dietary calories for half of humanity, is the first crop plant for which a high-quality reference genome sequence from a single variety was produced. We used resequencing microarrays to interrogate 100 Mb of the unique fraction of the reference genome for 20 diverse varieties and landraces that capture the impressive genotypic and phenotypic diversity of domesti...... hiện toàn bộ
Juvenile hormone acid methyltransferase: A key regulatory enzyme for insect metamorphosisProceedings of the National Academy of Sciences of the United States of America - Tập 100 Số 21 - Trang 11986-11991 - 2003
Tetsuro Shinoda, Kyo Itoyama
Juvenile hormone (JH) acid methyltransferase (JHAMT) is an enzyme that converts JH acids or inactive precursors of JHs to active JHs at the final step of JH biosynthesis pathway in insects. By fluorescent mRNA differential display, we have cloned a cDNA encoding JHAMT from the corpora allata (CA) of the silkworm,Bombyx mori(BmJHAMT). Th...... hiện toàn bộ
Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers.Proceedings of the National Academy of Sciences of the United States of America - Tập 86 Số 16 - Trang 6196-6200 - 1989
Thomas D. Kocher, W. Kelley Thomas, Axel Meyer, Scott V. Edwards, Svante Pääbo, Francis X. Villablanca, A C Wilson
With a standard set of primers directed toward conserved regions, we have used the polymerase chain reaction to amplify homologous segments of mtDNA from more than 100 animal species, including mammals, birds, amphibians, fishes, and some invertebrates. Amplification and direct sequencing were possible using unpurified mtDNA from nanogram samples of fresh specimens and microgram amounts of...... hiện toàn bộ
Multiple risk pathways for schizophrenia converge in serine racemase knockout mice, a mouse model of NMDA receptor hypofunctionProceedings of the National Academy of Sciences of the United States of America - Tập 110 Số 26 - 2013
Darrick T. Balu, Yan Li, Matthew D. Puhl, Michael A. Benneyworth, Alo C. Basu, Shunsuke Takagi, Vadim Y. Bolshakov, Joseph T. Coyle
SignificanceWe sought to determine whether the diverse hippocampal neuropathology observed in schizophrenia could be recapitulated in an animal model of NMDA receptor (NMDAR) hypofunction. Serine racemase-deficient (SR−/−) mice, which lack one of the NMDAR coagonistsd-serine, display impaired hippocampal plasticity, as well a...... hiện toàn bộ
Depurination causes mutations in SOS-induced cells.Proceedings of the National Academy of Sciences of the United States of America - Tập 78 Số 3 - Trang 1773-1777 - 1981
Roel M. Schaaper, Lawrence A. Loeb
Introduction of apurinic sites into phi X174 am3 DNA leads to loss of biological activity when measured in a transfection assay. For single-stranded DNA, approximately one apurinic site constitutes a lethal hit; for double-stranded (RFI) DNA, approximately 3.5 hits per strand are lethal. When the reversion frequency of am3 DNA is measured, no increase due to depurination is observed above ...... hiện toàn bộ
IL-15 mediates immune inflammatory hypernociception by triggering a sequential release of IFN-γ, endothelin, and prostaglandinProceedings of the National Academy of Sciences of the United States of America - Tập 103 Số 25 - Trang 9721-9725 - 2006
Waldiceu A. Verri, Thiago M. Cunha, Carlos Amílcar Parada, Xiao–Qing Wei, Sérgio Henrique Ferreira, Foo Y. Liew, Fernando Q. Cunha
IL-15 is closely associated with inflammatory diseases. IL-15 targeting is effective in treating experimental and clinical rheumatoid arthritis (RA). Because hyperalgesia accompanies RA, we investigated the ability of IL-15 to induced nociceptor sensitization (hypernociception). We report here that IL-15 induced time- and dose-dependent mechanical hypernociception in mice. IL-15-induced hy...... hiện toàn bộ
Temporal and spatial variation of the human microbiota during pregnancyProceedings of the National Academy of Sciences of the United States of America - Tập 112 Số 35 - Trang 11060-11065 - 2015
Daniel B. DiGiulio, Benjamin J. Callahan, Paul J. McMurdie, Elizabeth K. Costello, Deirdre J. Lyell, Anna Robaczewska, Christine Sun, Daniela S. Aliaga Goltsman, Ronald J. Wong, Gary M. Shaw, David K. Stevenson, Susan Holmes, David A. Relman
Significance
The human indigenous microbial communities (microbiota) play critical roles in health and may be especially important for mother and fetus during pregnancy. Using a case-control cohort of 40 women, we characterized weekly variation in the vaginal, gut, and oral microbiota during and after pregnancy. Microbiota membership remained relatively s...... hiện toàn bộ