Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia

Proceedings of the National Academy of Sciences of the United States of America - Tập 99 Số 24 - Trang 15524-15529 - 2002
George A. Călin1, Calin Dan Dumitru1, Masayoshi Shimizu1, Roberta Bichi1, Simona Zupo1, Evan Noch1, Hansjuerg Aldler1, Sashi Rattan1, Michael J. Keating1, R. Kanti1, Laura Z. Rassenti1, Thomas Enzler1, Massimo Negrini1, Florencia Bullrich1, Carlo M. Croce1
1Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107 USA; Clinical Immunology, National Institute for Research on Cancer, 16132 Genoa, Italy Europe; Department of Leukemia, University of Texas M. D. Anderson Cancer Center, Houston, TX 77030 USA; Long Island Jewish Medical Center, New Hyde Park, NY 11040 USA; and Department of Medicine, University of California at San Diego, La Jolla, CA 92093 USA

Tóm tắt

Micro-RNAs ( miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors (≈70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority (≈68%) of CLL cases.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 99 Số 24

15524-15529

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