Food ScienceNutrition and DieteticsBiotechnologyAgronomy and Crop Science
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Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, and perspectives in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. The journal focuses on hypothesis-driven research studies suitable for an international audience. Studies of local products or descriptive studies are discouraged.
Angharad M. R. Gatehouse, David S Hoe, Joyce E Flemming, V. A. Hilder, John A. Gatehouse
AbstractMature seeds of the inged bean (Psophocarpus tetragonolobus) are toxic to developing larvae of a range of cosmopolitan storage Bruchidae of economic importance, including the copea seed eevil, Callosobruchus maculatus. Insect feeding trials ere carried out in hich protein fractions from seeds of inged bean ere incorporated at a range of concentrations into artificial seeds, and their effects upon development of C maculatus determined. Both albumin and globulin fractions ere toxic to the developing larvae and their toxicity correlated ith their haemagglutinating activity. Assay of Psophocarpin A, B and C fractions demonstrated Psophocarpin B to be the most insecticidal and to contain the highest haemagglutinating activity. Purified basic seed lectin as highly insecticidal to C maculatus larvae, ith an LC50 value of c. 3·5 g kg−1. The physiological level of this protein in inged bean seeds is sufficient to account for their resistance to attack by C maculatus. inged bean trypsin inhibitor as also purified and tested in artificial seeds against C maculatus. Hoever, even at concentrations in excess of tice the physiological concentration it had no deleterious effects upon development.
Angharad M. R. Gatehouse, Frances M. Dewey, John A. Dove, Katherine A. Fenton, Árpád Pusztai
AbstractSeeds of the kidney bean (Phaseolus vulgaris) are toxic to developing larvae of the bruchid beetle (Callosobruchus maculatus), a major storage pest of many legumes. Insect feeding trials were carried out whereby the albumin and globulin protein fractions from seeds of P. vulgaris were incorporated into artificial seeds. Both fractions were shown to be toxic and to contain haemagglutinating activity, implicating the seed lectins as being involved in seed resistance. Further feeding trials using different P. vulgaris lectin preparations confirmed the toxicity of these lectins and suggested that it was the E‐type lectin subunits (erythrocyte‐binding) which were the major antimetabolites. Indirect immunofluorescence investigations using monospecific antisera for globulin lectins showed that the lectins, when ingested by the larvae, bound to the midgut epithelial cells. It was suggested that the mechanism of lectin toxicity in this instance is analogous to that known to occur in the rat, namely that the ingested lectin causes disruption of the epithelial cells of the larval midgut leading to breakdown of the transport of nutrients into these cells, and the absorption of potentially harmful substances. This is the first time that evidence for the mechanism of lectin toxicity has been obtained in insects.
AbstractThe free sugars and non‐volatile organic acids in Chinese gooseberry fruit were extracted and separated by lead salt and ion‐exchange procedures. Sugars and acids were identified and quantitatively determined by gas‐liquid chromatography (g.l.c.) of their trimethylsilyl (TMS) derivatives. Glucose, fructose, sucrose and trace amounts of sorbitol accounted for the total sugars. Citric, quinic and malic are the major acids, the outstanding feature being the high content of quinic acid in mature fruit. Small amounts of phosphoric, ascorbic, glucuronic, galacturonic, oxalic, succinic, fumaric, oxalacetic and p‐coumaric acids are also present. Qualitative and quantitative analysis of acids prepared as lead salts agreed closely with the determination of acids isolated by the ion‐exchange procedure. Identifications of sugars and acids were confirmed by paper and thin‐layer chromatography. Reducing sugars, total sugars and titratable acidity were determined by AOAC methods and the results compared with those from g.l.c.
AbstractA rapid and simple procedure for the separation, identification and quantitative analysis of common fruit sugars and non‐volatile organic acids has been developed. Acids were precipitated as their lead salts from fruit ethanolic extracts and the sugars in the remaining supernatant and washings were partitioned into aqueous methanol. These preparations with internal standards were dried and converted to their trimethylsilyl derivatives for analysis by gas‐liquid chromatography. In model studies, conditions which permitted full recovery of sugars from the lead salts of acids, while minimising the loss of acids, were investigated. For fruit acids reported present in measurable amounts (10 mg/100 g of fruit), replicate analysis of aliquots from the same extract was within ±5% for malonic, phosphoric, succinic, malic, citric, ascorbic and galacturonic acids and within ±12% for quinic acid. The use of the method, its advantages and limitations, in determining individual and total sugars and acids is illustrated on some New Zealand grown fruit (New Zealand grapefruit, mountain pawpaw, tangelo, apricot). Total sugars and total acids determined by this procedure gave comparable results with those obtained by using standard A.O.A.C. procedures.