Journal of Separation Science

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Simple 2D‐HPLC using a monolithic silica column for peptide separation
Journal of Separation Science - Tập 27 Số 10-11 - Trang 897-904 - 2004
Hiroshi Kimura, Tetsuya Tanigawa, Hironobu Morisaka, Tohru Ikegami, Ken Hosoya, N. Ishizuka, Hiroyoshi Minakuchi, Kazuki Nakanishi, Mitsuyoshi Ueda, Karin Cabrera, Nobuo Tanaka
AbstractSeparation of peptides by fast and simple two‐dimensional (2D)‐HPLC was studied using a monolithic silica column as a second‐dimension (2nd‐D) column. Every fraction from the first column, 5 cm long (2.1 mm ID) packed with polymer‐based cation exchange beads, was subjected to separation in the 2nd‐D using an octadecylsilylated (C18) mon...... hiện toàn bộ
Mixed‐mode hydrophilic interaction/cation‐exchange chromatography: Separation of complex mixtures of peptides of varying charge and hydrophobicity
Journal of Separation Science - Tập 31 Số 9 - Trang 1573-1584 - 2008
Colin T. Mant, Robert S. Hodges
AbstractMixed‐mode hydrophilic interaction/cation‐exchange chromatography (HILIC/CEX) was applied to the separation of two mixtures of synthetic peptide standards: (i) a 27‐peptide mixture containing three groups of peptides (each group containing nine peptides of the same net charge of +1, +2 or +3), where the hydrophilicity/hydrophobicity of adjacent peptides wit...... hiện toàn bộ
Methacrylate monolithic columns for capillary liquid chromatography polymerized using ammonium peroxodisulfate as initiator
Journal of Separation Science - Tập 26 Số 18 - Trang 1623-1628 - 2003
Petra Holdšvendová, Pavel Coufal, Jana Suchánková, Eva Tesařová, Zuzana Bosáková
AbstractFour methacrylate ester‐based monolithic columns for capillary liquid chromatography (CLC) were prepared by radical polymerization with ammonium peroxodisulfate (3 columns) and by thermal initiation (1 column). The polymerization mixture consisted of butyl methacrylate (BMA) and ethylene glycol dimethacrylate (EDMA), propan‐1‐ol, butane‐1,4‐diol, water, and...... hiện toàn bộ
Optimization of the porous structure and polarity of polymethacrylate‐based monolithic capillary columns for the LC‐MS separation of enzymatic digests
Journal of Separation Science - Tập 30 Số 17 - Trang 2814-2820 - 2007
Sebastiaan Eeltink, Laurent Geiser, František Švec, Jean M. J. Fréchet
AbstractThe porous structure as well as the polarity of methacrylate ester‐based monolithic stationary phases has been optimized to achieve the separation of various peptides originating from enzymatic digestion. The porous structure, determined by the size of both pores and microglobules, was varied through changes in the composition of porogenic solvents in the p...... hiện toàn bộ
Separation and determination of five major opium alkaloids with mixed mode of hydrophilic/cation‐exchange monolith by pressurized capillary electrochromatography
Journal of Separation Science - Tập 30 Số 17 - Trang 3011-3017 - 2007
Xucong Lin, Jiabin Wang, Liyu Li, Xiaochun Wang, Lu Huang, Zenghong Xie
AbstractA method for the separation and determination of five major opium alkaloids (narcotine, papaverine, thebaine, codeine, and morphine) in pericarpium papaveris by pressurized CEC (pCEC) with monolithic column has been developed. Under the optimum condition, linear calibration ranges of narcotine, papaverine, thebaine, codeine, and morphine were obtained as 2–...... hiện toàn bộ
Effects of functional monomers on retention behavior of small and large molecules in monolithic capillary columns at isocratic and gradient conditions
Journal of Separation Science - Tập 34 Số 16-17 - Trang 2054-2062 - 2011
Jiřı́ Urban, Pavel Jandera, Pavel Langmaier
AbstractThe polarity of (poly)methacrylate monolithic capillary columns was varied by using alkylmethacrylate monomers with butyl, cyclohexyl, 2‐ethylhexyl, lauryl, and stearyl functional groups in the polymerization mixture. The hydrodynamic properties, as well as the retention characteristics in RP‐LC of small molecules (alkylbenzenes) and of proteins under gradi...... hiện toàn bộ
Characterization of polymer monolithic stationary phases for capillary HPLC
Journal of Separation Science - Tập 26 Số 11 - Trang 1005-1016 - 2003
Dana Moravcová, Pavel Jandera, Jiřı́ Urban, Josef Planeta
AbstractMonolithic capillary columns have been prepared in fused‐silica capillaries by radical co‐polymerization of ethylene dimethacrylate and butyl methacrylate in the presence of porogen solvent mixtures containing various concentration ratios of 1‐propanol, 1,4‐butanediol, and water with azobisisobutyronitrile as the initiator of the polymerization reaction. Th...... hiện toàn bộ
Molecular imprinting of natural flavonoid antioxidants: Application in solid‐phase extraction for the sample pretreatment of natural products prior to HPLC analysis
Journal of Separation Science - Tập 29 Số 15 - Trang 2310-2321 - 2006
Georgios Theodoridis, Martina Lasáková, Veronika Škeříková, Andromachi Tegou, Nicoletta Giantsiou, Pavel Jandera
AbstractAs shown in the past years, SPE based on molecularly imprinted polymers (MIPs) may provide significant enhancement of selectivity in sample preparation and analyte preconcentration. The objective of this work was the fabrication of MIPs for the specific adsorption of rutin and quercetin. The two flavonoids were used as the template molecules for the prepara...... hiện toàn bộ
Comparison of monolithic silica and polymethacrylate capillary columns for LC
Journal of Separation Science - Tập 27 Số 10-11 - Trang 789-800 - 2004
Dana Moravcová, Pavel Jandera, Jiřı́ Urban, Josef Planeta
AbstractOrganic polymer monolithic capillary columns were prepared in fused‐silica capillaries by radical co‐polymerization of ethylene dimethacrylate and butyl methacrylate monomers with azobisisobutyronitrile as initiator of the polymerization reaction in the presence of various amounts of porogenic solvent mixtures and different concentration ratios of monomers ...... hiện toàn bộ
Separation properties of novel and commercial polar stationary phases in hydrophilic interaction and reversed‐phase liquid chromatography mode
Journal of Separation Science - Tập 31 Số 9 - Trang 1492-1503 - 2008
Junyan Wu, Wolfgang Bicker, Wolfgang Lindner
AbstractFour novel nonionic polar stationary phases were synthesised by anchoring first 2‐mercaptoethanol and 1‐thioglycerol, respectively, onto vinylised silica (ME and TG packings) followed by an on‐phase oxidation with excess hydrogen peroxide in aqueous medium which yielded sulphoxide analogues of the embedded sulphide groups, i. e. o...... hiện toàn bộ
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