Simple 2D‐HPLC using a monolithic silica column for peptide separationJournal of Separation Science - Tập 27 Số 10-11 - Trang 897-904 - 2004
Hiroshi Kimura, Tetsuya Tanigawa, Hironobu Morisaka, Tohru Ikegami, Ken Hosoya, N. Ishizuka, Hiroyoshi Minakuchi, Kazuki Nakanishi, Mitsuyoshi Ueda, Karin Cabrera, Nobuo Tanaka
AbstractSeparation of peptides by fast and simple two‐dimensional (2D)‐HPLC was studied using a monolithic silica column as a second‐dimension (2nd‐D) column. Every fraction from the first column, 5 cm long (2.1 mm ID) packed with polymer‐based cation exchange beads, was subjected to separation in the 2nd‐D using an octadecylsilylated (C18) mon...... hiện toàn bộ
Methacrylate monolithic columns for capillary liquid chromatography polymerized using ammonium peroxodisulfate as initiatorJournal of Separation Science - Tập 26 Số 18 - Trang 1623-1628 - 2003
Petra Holdšvendová, Pavel Coufal, Jana Suchánková, Eva Tesařová, Zuzana Bosáková
AbstractFour methacrylate ester‐based monolithic columns for capillary liquid chromatography (CLC) were prepared by radical polymerization with ammonium peroxodisulfate (3 columns) and by thermal initiation (1 column). The polymerization mixture consisted of butyl methacrylate (BMA) and ethylene glycol dimethacrylate (EDMA), propan‐1‐ol, butane‐1,4‐diol, water, and...... hiện toàn bộ
Separation and determination of five major opium alkaloids with mixed mode of hydrophilic/cation‐exchange monolith by pressurized capillary electrochromatographyJournal of Separation Science - Tập 30 Số 17 - Trang 3011-3017 - 2007
Xucong Lin, Jiabin Wang, Liyu Li, Xiaochun Wang, Lu Huang, Zenghong Xie
AbstractA method for the separation and determination of five major opium alkaloids (narcotine, papaverine, thebaine, codeine, and morphine) in pericarpium papaveris by pressurized CEC (pCEC) with monolithic column has been developed. Under the optimum condition, linear calibration ranges of narcotine, papaverine, thebaine, codeine, and morphine were obtained as 2–...... hiện toàn bộ
Characterization of polymer monolithic stationary phases for capillary HPLCJournal of Separation Science - Tập 26 Số 11 - Trang 1005-1016 - 2003
Dana Moravcová, Pavel Jandera, Jiřı́ Urban, Josef Planeta
AbstractMonolithic capillary columns have been prepared in fused‐silica capillaries by radical co‐polymerization of ethylene dimethacrylate and butyl methacrylate in the presence of porogen solvent mixtures containing various concentration ratios of 1‐propanol, 1,4‐butanediol, and water with azobisisobutyronitrile as the initiator of the polymerization reaction. Th...... hiện toàn bộ
Comparison of monolithic silica and polymethacrylate capillary columns for LCJournal of Separation Science - Tập 27 Số 10-11 - Trang 789-800 - 2004
Dana Moravcová, Pavel Jandera, Jiřı́ Urban, Josef Planeta
AbstractOrganic polymer monolithic capillary columns were prepared in fused‐silica capillaries by radical co‐polymerization of ethylene dimethacrylate and butyl methacrylate monomers with azobisisobutyronitrile as initiator of the polymerization reaction in the presence of various amounts of porogenic solvent mixtures and different concentration ratios of monomers ...... hiện toàn bộ