Journal of Separation Science

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Synthesis and application of novel phenylboronate affinity materials based on organic polymer particles for selective trapping of glycoproteins
Journal of Separation Science - Tập 32 Số 10 - Trang 1673-1685 - 2009
Beatrix Preinerstorfer, Michael Lämmerhofer, Wolfgang Lindner
AbstractWe report on synthesis concepts for the fabrication of various novel phenylboronate affinity materials based on polymethacrylate epoxy beads (Fractogel® EMD Epoxy (M) 40–90 μm) and the testing of these functionalized polymer particles for selective trapping of a glycoprotein from a standard mixture containing a glycosylated and a nonglycosylated protein. Tw...... hiện toàn bộ
Standard‐free kinetic calibration for rapid on‐site analysis by solid‐phase microextraction
Journal of Separation Science - Tập 31 Số 6-7 - Trang 1167-1172 - 2008
Gangfeng Ouyang, Jibao Cai, Xu Zhang, Hui Li, Janusz Pawliszyn
AbstractIn this study, a new calibration method, standard‐free kinetic calibration, is proposed for rapid on‐site analysis by solid‐phase microextraction (SPME), based on the diffusion‐controlled mass transfer model and equilibrium extraction. With this calibration method, all analytes can be directly calibrated with only two samplings. The feasibility of this cali...... hiện toàn bộ
Preparative isolation of pseudolaric acids A and B, and their glucosides from the root bark of Pseudolarix kaempferi using high‐speed counter‐current chromatography
Journal of Separation Science - Tập 32 Số 2 - Trang 309-313 - 2009
Quan‐Bin Han, Lina Wong, Fanny Lai, Nian‐Yun Yang, Jing‐Zheng Song, Chun‐Feng Qiao, Hong‐Xi Xu
AbstractIn order to provide the chemical markers for the quality control of herbal medicines, four diterpenoids, pseudolaric acids A and B (PAA and PAB), and their glucosides were isolated from the methanol extract of the Chinese herb Pseudolarix kaempferi using high‐speed counter‐current chromatography (HSCCC). The diphase solvent system...... hiện toàn bộ
Comparison of separation power of ultra performance liquid chromatography and comprehensive two‐dimensional liquid chromatography in the separation of phenolic compounds in beverages
Journal of Separation Science - Tập 31 Số 19 - Trang 3466-3472 - 2008
Maarit Kivilompolo, Tuulia Hyötyläinen
AbstractIn this study, 1‐D and 2‐D liquid chromatographic systems, namely, conventional HPLC, UPLC, HPLC×HPLC and HPLC×UPLC systems were developed and evaluated for the separation of phenolic acids in wine and juices. In the LC×LC studies, the first dimension separation was based on RPLC and the second dimension was performed with ion‐pair chromatography. Three dif...... hiện toàn bộ
Simple 2D‐HPLC using a monolithic silica column for peptide separation
Journal of Separation Science - Tập 27 Số 10-11 - Trang 897-904 - 2004
Hiroshi Kimura, Tetsuya Tanigawa, Hironobu Morisaka, Tohru Ikegami, Ken Hosoya, N. Ishizuka, Hiroyoshi Minakuchi, Kazuki Nakanishi, Mitsuyoshi Ueda, Karin Cabrera, Nobuo Tanaka
AbstractSeparation of peptides by fast and simple two‐dimensional (2D)‐HPLC was studied using a monolithic silica column as a second‐dimension (2nd‐D) column. Every fraction from the first column, 5 cm long (2.1 mm ID) packed with polymer‐based cation exchange beads, was subjected to separation in the 2nd‐D using an octadecylsilylated (C18) mon...... hiện toàn bộ
Mixed‐mode hydrophilic interaction/cation‐exchange chromatography: Separation of complex mixtures of peptides of varying charge and hydrophobicity
Journal of Separation Science - Tập 31 Số 9 - Trang 1573-1584 - 2008
Colin T. Mant, Robert S. Hodges
AbstractMixed‐mode hydrophilic interaction/cation‐exchange chromatography (HILIC/CEX) was applied to the separation of two mixtures of synthetic peptide standards: (i) a 27‐peptide mixture containing three groups of peptides (each group containing nine peptides of the same net charge of +1, +2 or +3), where the hydrophilicity/hydrophobicity of adjacent peptides wit...... hiện toàn bộ
Preparation and HPLC applications of rigid macroporous organic polymer monoliths
Journal of Separation Science - Tập 27 Số 10-11 - Trang 747-766 - 2004
František Švec
AbstractRigid porous polymer monoliths are a new class of materials that emerged in the early 1990s. These monolithic materials are typically prepared using a simple molding process carried out within the confines of a closed mold. For example, polymerization of a mixture comprising monomers, free‐radical initiator, and porogenic solvent affords macroporous materia...... hiện toàn bộ
HPLC retention behavior on hydride‐based stationary phases
Journal of Separation Science - Tập 30 Số 5 - Trang 637-647 - 2007
Joseph J. Pesek, Maria T. Matyska, Susan Larrabee
AbstractTwo stationary phases attached to a silica hydride surface, cholesterol and bidentate C18, are investigated with a number of pharmaceutically related compounds in order to illustrate the various retention mechanisms that are possible for these bonded materials. The test solutes range from hydrophilic to hydrophobic based on log P ...... hiện toàn bộ
Methacrylate monolithic columns for capillary liquid chromatography polymerized using ammonium peroxodisulfate as initiator
Journal of Separation Science - Tập 26 Số 18 - Trang 1623-1628 - 2003
Petra Holdšvendová, Pavel Coufal, Jana Suchánková, Eva Tesařová, Zuzana Bosáková
AbstractFour methacrylate ester‐based monolithic columns for capillary liquid chromatography (CLC) were prepared by radical polymerization with ammonium peroxodisulfate (3 columns) and by thermal initiation (1 column). The polymerization mixture consisted of butyl methacrylate (BMA) and ethylene glycol dimethacrylate (EDMA), propan‐1‐ol, butane‐1,4‐diol, water, and...... hiện toàn bộ
Optimization of the porous structure and polarity of polymethacrylate‐based monolithic capillary columns for the LC‐MS separation of enzymatic digests
Journal of Separation Science - Tập 30 Số 17 - Trang 2814-2820 - 2007
Sebastiaan Eeltink, Laurent Geiser, František Švec, Jean M. J. Fréchet
AbstractThe porous structure as well as the polarity of methacrylate ester‐based monolithic stationary phases has been optimized to achieve the separation of various peptides originating from enzymatic digestion. The porous structure, determined by the size of both pores and microglobules, was varied through changes in the composition of porogenic solvents in the p...... hiện toàn bộ
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