Preparative isolation of pseudolaric acids A and B, and their glucosides from the root bark of Pseudolarix kaempferi using high‐speed counter‐current chromatography

Journal of Separation Science - Tập 32 Số 2 - Trang 309-313 - 2009
Quan‐Bin Han1, Lina Wong, Fanny Lai, Nian‐Yun Yang, Jing‐Zheng Song, Chun‐Feng Qiao, Hong‐Xi Xu
1Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Shatin, N. T., Hong Kong, China

Tóm tắt

AbstractIn order to provide the chemical markers for the quality control of herbal medicines, four diterpenoids, pseudolaric acids A and B (PAA and PAB), and their glucosides were isolated from the methanol extract of the Chinese herb Pseudolarix kaempferi using high‐speed counter‐current chromatography (HSCCC). The diphase solvent system was n‐hexane/EtOAc/MeOH/H2O which was used at two ratios (5:5:5:5 and 1:9:4:6 by volume) in the separation of pseudolaric acids and their glycosides, respectively. As a result, PAA (14 mg), PAB (129 mg), PAA‐O‐β‐D‐glucopyranoside (8 mg, PAAG), and PAB‐O‐β‐D‐glucopyranoside (42 mg, PABG) were obtained from 0.5 g of the crude extract. Their purities were determined to be above 97% by HPLC analysis. Their chemical structures were confirmed by 1H and 13C NMR analysis or HPLC comparison with the reference compounds.

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Thông tin xuất bản

Journal of Separation Science

Tập 32 Số 2

309-313

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