Comparison of separation power of ultra performance liquid chromatography and comprehensive two‐dimensional liquid chromatography in the separation of phenolic compounds in beverages

Journal of Separation Science - Tập 31 Số 19 - Trang 3466-3472 - 2008
Maarit Kivilompolo1, Tuulia Hyötyläinen
1Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Helsinki, Finland

Tóm tắt

AbstractIn this study, 1‐D and 2‐D liquid chromatographic systems, namely, conventional HPLC, UPLC, HPLC×HPLC and HPLC×UPLC systems were developed and evaluated for the separation of phenolic acids in wine and juices. In the LC×LC studies, the first dimension separation was based on RPLC and the second dimension was performed with ion‐pair chromatography. Three different columns, namely two short columns packed with either 2.5 or 1.7 μm particles and a monolithic column, were tested for the fast second dimension separation. The best results were obtained when the monolithic column was applied for the second dimension separation. The peak capacities for comprehensive 2‐D systems varied from 330 to 616.

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Thông tin xuất bản

Journal of Separation Science

Tập 31 Số 19

3466-3472

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