Journal of Plant Biochemistry and Biotechnology

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Development of a Specific Detection Technique for Cowpea Golden Mosaic Virus
Journal of Plant Biochemistry and Biotechnology - Tập 13 - Trang 131-133 - 2012
Anirban Roy, V. G. Malathi
Whitefly transmitted begomoviruses are on increase in tropical and subtropical countries. It is especially true of yellow mosaic viruses infecting legumes. Management of viral diseases requires specific detection techniques to demonstrate their presence in several other alternative and weed hosts. With this in view, nucleic acid hybridization test to detect Cowpea golden mosaic virus (CPGMV) in ma... hiện toàn bộ
Large-scale plantlets conversion from cotyledonary somatic embryos of Kalopanax septemlobus tree using bioreactor cultures
Journal of Plant Biochemistry and Biotechnology - Tập 20 - Trang 241-248 - 2011
Sun Ja Kim, Yaser Hassan Dewir, Heung Kyu Moon
A suitable bioreactor system for large scale embryo-to-plantlets conversion of Kalopanax septemlobus was established. In temporary immersion with net (TIN) bioreactor, 85% of embryos successfully produced plantlets whereas in continuous immersion with net (CIN) bioreactor, only conversion rate of 29.3% was obtained. Embryos cultured in TIN bioreactor produced more vigorous plantlets in terms of fr... hiện toàn bộ
Analysis of activity driven by upstream regulatory modules (URM) of tapetum specific genes TA29 and A9 at ectopic locations in tobacco transgenics
Journal of Plant Biochemistry and Biotechnology - Tập 27 - Trang 443-452 - 2018
Preeti Apurve Sharma, Neetu Verma, Pradeep Kumar Burma
TA29 and A9 are genes from Nicotiana tabacum and Arabidopsis thaliana respectively, which express in a tapetum specific manner. The upstream regulatory modules (URMs; i.e. the promoter and the 5′UTR) of these genes have been used in development of male sterile and restorer lines expressing the barnase and barstar genes for hybrid seed production. While initial studies show that these URMs drive th... hiện toàn bộ
Wheat blast disease management: cues from the advancements in molecular biology of rice-Magnaporthe pathosystem
Journal of Plant Biochemistry and Biotechnology - Tập 27 - Trang 249-259 - 2017
B. N. Devanna, T. R. Sharma
Wheat blast disease, caused by Magnaporthe oryzae Triticum pathotype, was first reported in 1985 from Brazil, and since then it has emerged as a major threat to global wheat production. Unlike rice blast disease, where many rice lines are known to provide nearly complete resistance to M. oryzae, the resistance response of wheat germplasm to blast pathogen is rather limited to a few isolated lines ... hiện toàn bộ
In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit
Journal of Plant Biochemistry and Biotechnology - Tập 24 - Trang 129-134 - 2013
Nguyen Hoang Loc, Nguyen Van Song, Dang Thanh Long, Tae-Geum Kim, Moon-Sik Yang
In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkably different from the wild-type. Their physiological and biochemical characteristics are relatively different... hiện toàn bộ
Characterization of two novel drought responsive genes in wheat
Journal of Plant Biochemistry and Biotechnology - Tập 29 - Trang 78-85 - 2019
Iffat Kiani, S. M. Saqlan Naqvi, Tasawar Sultana, Fariha Khan
Drought stress modulates the expression levels of numerous stress responsive genes which are not yet independently characterized in detail. Isolation and molecular characterization of the novel genes is necessary to understand plant stress physiology and to engineer stress resistant crops. In this study, two drought responsive genes, wheat cold-regulated gene 410c (WCOR410C) and Triticum aestivum ... hiện toàn bộ
Micropropagation of Withania coagulans (Stocks) Dunal: A Critically Endangered Medicinal Herb
Journal of Plant Biochemistry and Biotechnology - Tập 18 - Trang 249-252 - 2009
Rohit Jain, Arunima Sinha, Sumita Kachhwaha, S. L. Kothari
An efficient micropropagation protocol has been developed for Withania coagulans, a highly endangered medicinal herb and an important natural source of withanolides. Prolific multiplication of axillary buds occurred from the nodal segments taken from adult plant, and cultured on MS medium enriched with BA (0.5 mg l−1), Kn (0.5 mg l−1) and PG (0.5 mg l−1). Nodal segments and shoot tips of elongated... hiện toàn bộ
Metageographic population analysis of Colletotrichum truncatum associated with chili fruit rot and other hosts using ITS region nucleotide sequences
Journal of Plant Biochemistry and Biotechnology - Tập 25 - Trang 64-72 - 2015
Abhishek Katoch, Chandra S. Prabhakar, Prem N. Sharma
Colletotrichum truncatum is one of the most economically important fungal pathogen causing anthracnose disease in pre and post-harvest stages of many crops worldwide. Little information is available in the literature on the genetic analysis and demographic history of this fungal pathogen. In the present study nucleotide sequence data of internal transcribed spacer (ITS) region were analyzed for C.... hiện toàn bộ
Acid Lipase of Castor Bean Lipid Bodies : Isolation and Characterisation
Journal of Plant Biochemistry and Biotechnology - Tập 6 - Trang 13-18 - 2012
A. Altaf, T. V. Ankers, N. Kaderbhai, E. I. Mercer, M. A. Kaderbhai
The acid lipase of castor endosperm lipid bodies has been studied using colorimetric assay based on the measure of the hydrolytic activity of p-nitrophenyl ester of palmitate and other acyl derivatives. These substrates are compatible with the natural triacylglycerols for the measure of lipolytic activities. The subcellularly-surveyed acid lipolytic activity in the germinated castor bean endosperm... hiện toàn bộ
Transient expression of the homogentisate phytyltransferase gene from Clitoria ternatea causes metabolic enhancement of α-tocopherol biosynthesis and chlorophyll degradation in tomato leaves
Journal of Plant Biochemistry and Biotechnology - Tập 27 - Trang 55-67 - 2017
Thaniya Wunnakup, Sornkanok Vimolmangkang, Wanchai De-Eknamkul
Homogentisate prenyltransferase (HPT) is an important enzyme involved in the α-tocopherol (vitamin E) biosynthetic pathway of all plant taxa. Tocopherol biosynthesis and chlorophyll degradation are related, but more information is needed to explain their relationship. In this study, a candidate gene for HPT from Clitoria ternatea (CtHPT) was isolated and identified via a phylogeny-based approach, ... hiện toàn bộ
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