Journal of Clinical Laboratory Analysis
SCIE-ISI SCOPUS (1987-2025)
0887-8013
1098-2825
Mỹ
Cơ quản chủ quản: Wiley-Liss Inc. , WILEY
Lĩnh vực:
Public Health, Environmental and Occupational HealthHematologyImmunology and AllergyMicrobiology (medical)Biochemistry (medical)Medical Laboratory TechnologyClinical Biochemistry
Các bài báo tiêu biểu
Multiplex polymerase chain reaction: A practical approach
Tập 16 Số 1 - Trang 47-51 - 2002
AbstractConsiderable time and effort can be saved by simultaneously amplifying
multiple sequences in a single reaction, a process referred to as multiplex
polymerase chain reaction (PCR). Multiplex PCR requires that primers lead to
amplification of unique regions of DNA, both in individual pairs and in
combinations of many primers, under a single set of reaction conditions. In
addition, methods mu...
Checklist for optimization and validation of real‐time PCR assays
Tập 23 Số 3 - Trang 145-151 - 2009
AbstractReal‐time polymerase chain reaction (PCR) is a frequently used technique
in molecular diagnostics. To date, practical guidelines for the complete process
of optimization and validation of commercial and in‐house developed molecular
diagnostic methods are scare. Therefore, we propose a practical guiding
principle for the optimization and validation of real‐time PCR assays. Based on
literatu...
Review of in vivo pharmacokinetics and toxicology of phosphorothioate oligonucleotides
Tập 9 Số 2 - Trang 129-137 - 1995
What are the cut‐off levels for IL‐6 and CRP in neonatal sepsis?
Tập 24 Số 6 - Trang 407-412 - 2010
AbstractNeonatal sepsis, characterized by systemic signs of infection in the
first month of life, remains an important clinical syndrome. Despite advances in
neonatology, it has high rates of mortality and morbidity. The combine or alone
usage of interleukin‐6 (IL‐6) and C‐reactive protein (CRP) has recently been
proven to be useful in the early diagnosis of sepsis in newborns. The study
included ...
Prostate‐specific antigen and prostate gland volume: Correlation and clinical application
Tập 4 Số 2 - Trang 135-137 - 1990
AbstractWe studied 103 patients seen in our Prostate Cancer Detection Clinic to
determine whether a correlation exists between serum prostate‐specific antigen
(PSA) values and ultrasound‐calculated prostate gland volume. Seventy men (68%)
had a PSA value ≤ ng/ml (our upper limit of normal). The men were subclassified
by prostate gland volume at arbitrary break points. Twenty‐five men (24%) had a
p...
Clinical values of circular RNA 0000181 in the screening of gastric cancer
Tập 32 Số 4 - 2018
BackgroundCircular RNAs (circRNAs) are recently found involved in cancer
occurrence and development. However, their values in the diagnosis of gastric
cancers are largely unknown. In this study, we analyzed the values of
hsa_circ_0000181 in the diagnosis of gastric cancer.MethodsUsing divergent
primers, hsa_circ_0000181 expression levels in fresh gastric cancer tissues and
paired adjacent non‐tumo...
Potential clinical applications of c‐reactive protein
Tập 4 Số 3 - Trang 231-235 - 1990
AbstractThis article reviews the clinical applications of C‐reactive protein
(CRP). This acute‐phase protein is a distinct and sensitive marker for
inflammation and tissue injury. It is a simple, fast, and relatively inexpensive
latex agglutination test. The aspects of CRP reviewed include diagnostic
support, serial measurements to evaluate disease course and therapeutic
response, and screening st...
Cholesterol oxides and carcinogenesis
Tập 5 Số 3 - Trang 219-225 - 1991
AbstractExperimental evidence indicates a relationship between cholesterol
α‐epoxide and skin cancer, and exposure of skin fibroblasts to ultraviolet
radiation enduces formation of significant levels of this oxide. Colon cancer is
also etiologically linked to cholesterol oxidation products. Higher than normal
levels of cholestanetriol have been found in patients with colon cancer and also
in those...
Detection of autoantibodies to ss‐a/ro by indirect immunofluorescence using a transfected and overexpressed human 60 kd ro autoantigen in hep‐2 cells
Tập 9 Số 3 - Trang 218-224 - 1995
AbstractThe objective of the study was to determine the sensitivity and
specificity of an indirect immunofluorescence (IIF) assay using transfected
HEp‐2 cells to detect anti‐SS‐A/Ro autoantibodies in human sera. Seventy‐three
sera having SS‐A/Ro autoantibodies as determined by double immunodiffusion (ID)
and immunoblotting (IB) were tested by IIF on a HEp‐2 cell substrate that had
been transfecte...
MPS screening methods, the berry spot and acid turbidity tests, cause a high incidence of false‐negative results in sanfilippo and morquio syndromes
Tập 16 Số 5 - Trang 253-258 - 2002
AbstractBecause of differences in the types and quantities of glycosaminoglycans
(GAGs) in various mucopolysaccharidoses (MPSs), MPS screening tests, including
the Berry spot and acid turbidity tests, are not specific or sensitive enough
for the preliminary diagnosis of MPS. A false‐negative result is common. We
analyzed urine samples collected from 492 patients who were examined for inborn
errors...