Clinical values of circular RNA 0000181 in the screening of gastric cancer

Journal of Clinical Laboratory Analysis - Tập 32 Số 4 - 2018
Qianfu Zhao1, Shijun Chen1, Tianwen Li1, Bingxiu Xiao1, Xinjun Zhang2
1Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, Ningbo, China
2Department of Gastroenterology, The Affiliated Hospital of Ningbo University School of Medicine, Ningbo, China

Tóm tắt

BackgroundCircular RNAs (circRNAs) are recently found involved in cancer occurrence and development. However, their values in the diagnosis of gastric cancers are largely unknown. In this study, we analyzed the values of hsa_circ_0000181 in the diagnosis of gastric cancer.MethodsUsing divergent primers, hsa_circ_0000181 expression levels in fresh gastric cancer tissues and paired adjacent non‐tumorous tissues, and plasmas from patient with gastric cancer and health people were detected by real‐time quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). The association between hsa_circ_0000181 levels and the clinicopathologic features of patients with gastric cancer was further analyzed. Finally, to evaluate the diagnostic value, receiver operating characteristic (ROC) curve was established.ResultsHsa_circ_0000181 levels in gastric cancer tissues and plasma from gastric cancer patients were significantly decreased than those in paired adjacent non‐tumorous tissues (P < .001) and healthy people (P < .001), respectively. Furthermore, hsa_circ_0000181 expression in gastric cancer tissues was significantly correlated with tumor diameter (P = .027), lymphatic metastasis (P = .044), distal metastasis (P = .023), and carbohydrate antigen 19‐9 (P = .031). Its decreased levels in patients' plasma were significantly associated with differentiation (P = .038) and carcinoembryonic antigen (P = .037). The areas under ROC curve were 0.756. The specificity of tissue hsa_circ_0000181 and sensitivity of plasma hsa_circ_0000181 were 85.2% and 99.0%, respectively.ConclusionsThanks to the high stability, tissue and plasma hsa_circ_0000181 may be a novel biomarker for the diagnosis of gastric cancer.

Từ khóa


Tài liệu tham khảo

10.1111/hepr.12871

10.1073/pnas.73.11.3852

10.1038/bjc.2016.451

10.1016/j.celrep.2014.10.062

10.3389/fgene.2015.00175

Wang J, 2017, Pretreatment platelet‐to‐lymphocyte ratio is associated with the response to first‐line chemotherapy and survival in patients with metastatic gastric cancer, J Clin Lab Anal

Xiang F, 2017, Association of 758 G/A polymorphism of 3′untranslated region of prohibitin with risk of gastric cancer, J Clin Lab Anal

10.18632/oncotarget.6926

10.1002/jcla.21987

10.5946/ce.2014.47.6.478

10.1002/jcla.21802

10.1002/cam4.1055

10.18632/oncotarget.9336

10.1016/j.cca.2015.02.018

10.1007/s13277-015-3903-3

10.1007/s13277-014-1709-3

10.1038/280339a0

10.1016/j.molcel.2013.08.017

10.1016/j.celrep.2014.12.002

10.1038/nsmb.2959

10.1371/journal.pone.0030733

Li J, 2015, Circular RNAs in cancer: novel insights into origins, properties, functions and implications, Am J Cancer Res, 5, 472

10.1038/nature11993

10.18632/oncotarget.12684

10.1073/pnas.0906277106

10.2337/db12-0451

10.1371/journal.pgen.1001233

10.1007/s101200200035

10.1007/s00432-016-2183-7

10.1097/00000658-199810000-00002

10.1007/s101200050006

10.5230/jgc.2015.15.1.19

Peng HX, 2016, Combination of preoperative NLR, PLR and CEA could increase the diagnostic efficacy for I‐III stage CRC, J Clin Lab Anal

Wu J, 2014, Circulating microRNA‐21 is a potential diagnostic biomarker in gastric cancer, Dis Markers, 2015, 435656

Thông tin
Thông tin xuất bản

Journal of Clinical Laboratory Analysis

Tập 32 Số 4

Thông tin tác giả