Metal ion reconstitution studies of yeast copper-zinc superoxide dismutase: the "phantom" subunit and the possible role of Lys7pJBIC Journal of Biological Inorganic Chemistry - Tập 3 - Trang 650-662 - 1998
Thomas J. Lyons, Aram Nersissian, Joy J. Goto, Haining Zhu, Edith Butler Gralla, Joan Selverstone Valentine
Using a corrected molar extinction coefficient for yeast apo copper-zinc superoxide dismutase (CuZnSOD), we have confirmed that the metal binding properties of this protein in vitro differ greatly from those of the bovine and human CuZnSOD enzymes. Thus yeast apo CuZnSOD was found to bind only one Co2+ per protein dimer under the conditions in which the bovine and human CuZnSOD apoenzymes readily...... hiện toàn bộ
Structural characterization of a carbon monoxide adduct of a heme–DNA complexJBIC Journal of Biological Inorganic Chemistry - Tập 17 - Trang 437-445 - 2011
Kaori Saito, Hulin Tai, Masashi Fukaya, Tomokazu Shibata, Ryu Nishimura, Saburo Neya, Yasuhiko Yamamoto
The structure of a carbon monoxide (CO) adduct of a complex between heme and a parallel G-quadruplex DNA formed from a single repeat sequence of the human telomere, d(TTAGGG), has been characterized using 1H and 13C NMR spectroscopy and density function theory calculations. The study revealed that the heme binds to the 3′-terminal G-quartet of the DNA though a π–π stacking interaction between the ...... hiện toàn bộ
Vanadyl bisacetylacetonate protects β cells from palmitate-induced cell death through the unfolded protein response pathwayJBIC Journal of Biological Inorganic Chemistry - Tập 16 - Trang 789-798 - 2011
Zhonglan Gao, Chengyue Zhang, Siwang Yu, Xiaoda Yang, Kui Wang
Endoplasmic reticulum (ER) stress induced by free fatty acids (FFA) is important to β-cell loss during the development of type 2 diabetes. To test whether vanadium compounds could influence ER stress and the responses in their mechanism of antidiabetic effects, we investigated the effects and the mechanism of vanadyl bisacetylacetonate [VO(acac)2] on β cells upon treatment with palmitate, a typica...... hiện toàn bộ
Metal ions in the structure and function of RNAJBIC Journal of Biological Inorganic Chemistry - Tập 7 - Trang 679-690 - 2002
Anna Pyle
It is becoming increasingly clear that RNA is more than a passive carrier of genetic information. Folded RNA molecules play key roles in almost every aspect of cellular metabolism, including protein transport, RNA splicing, peptide bond formation, and translational regulation. This is facilitated by the multifunctional nature of RNA biopolymers which can serve as rigid structural scaffolds, confo...... hiện toàn bộ
Interaction between cyanobacterial copper chaperone Atx1 and zinc homeostasisJBIC Journal of Biological Inorganic Chemistry - Tập 15 - Trang 77-85 - 2009
Samantha J. Dainty, Carl J. Patterson, Kevin J. Waldron, Nigel J. Robinson
Cyanobacterial Atx1 is a copper chaperone which interacts with two copper-transporting ATPases to assist copper supply to plastocyanin and cytochrome oxidase. ZiaA is a Zn2+-exporting ATPase and ziaA expression is regulated by ZiaR. Here we show that gene expression from the ziaA operator promoter, monitored using reverse transcriptase PCR and lacZ fusions, is elevated in Δatx1 mutants. Although C...... hiện toàn bộ
Copper-induced structural propensities of the amyloidogenic region of human prion proteinJBIC Journal of Biological Inorganic Chemistry - Tập 19 - Trang 635-645 - 2014
Caterina Migliorini, Adalgisa Sinicropi, Henryk Kozlowski, Marek Luczkowski, Daniela Valensin
Transmissible spongiform encephalopathies are associated with the misfolding of the cellular Prion Protein (PrPC) to an abnormal protein isoform, called scrapie prion protein (PrPSc). The structural rearrangement of the fragment of N-terminal domain of the protein spanning residues 91–127 is critical for the observed structural transition. The amyloidogenic domain of the protein encloses two coppe...... hiện toàn bộ