Interaction between cyanobacterial copper chaperone Atx1 and zinc homeostasis
Tóm tắt
Cyanobacterial Atx1 is a copper chaperone which interacts with two copper-transporting ATPases to assist copper supply to plastocyanin and cytochrome oxidase. ZiaA is a Zn2+-exporting ATPase and ziaA expression is regulated by ZiaR. Here we show that gene expression from the ziaA operator promoter, monitored using reverse transcriptase PCR and lacZ fusions, is elevated in Δatx1 mutants. Although Cu+ tightly binds recombinant ZiaR in vitro, Cu+ is less effective at dissociating ZiaR–DNA complexes than Zn2+ and crucially ziaA expression responds to Zn2+ but not copper in both wild-type and Δatx1 cells. Consistent with enhanced expression of ZiaA, Δatx1 cells have slightly elevated Zn2+ resistance. Recombinant Zn2+–Atx1 is recovered from Zn2+-supplemented Escherichia coli and even after copper supplementation substantial amounts of Zn2+–Atx1 are isolated. Taken together, these data suggest that Zn2+–Atx1 can form in vivo.
Tài liệu tham khảo
Lin SJ, Culotta VC (1995) Proc Natl Acad Sci USA 92:3784–3788
Lin SJ, Pufahl RA, Dancis A, O’Halloran TV, Culotta VC (1997) J Biol Chem 272:9215–9220
Pufahl RA, Singer CP, Peariso KL, Lin SJ, Schmidt PJ, Fahrni CJ, Culotta VC, Penner-Hahn JE, O’Halloran TV (1997) Science 278:853–856
Klomp LW, Lin SJ, Yuan DS, Klausner RD, Culotta VC, Gitlin JD (1997) J Biol Chem 272:9221–9226
Kim BE, Nevitt T, Thiele DJ (2008) Nat Chem Biol 4:176–185
Harrison MD, Jones CE, Dameron CT (1999) J Biol Inorg Chem 4:145–153
Wimmer R, Herrmann T, Solioz M, Wüthrich K (1999) J Biol Chem 274:22597–22603
Banci L, Bertini I, Del Conte R, Markey J, Ruiz-Dueñas FJ (2001) Biochemistry 40:15660–15668
Borrelly GPM, Blindauer CA, Schmid R, Butler CS, Cooper CE, Harvey I, Sadler PJ, Robinson NJ (2004) Biochem J 378:293–297
Banci L, Bertini I, Ciofi-Baffoni S, Su XC, Borrelly GP, Robinson NJ (2004) J Biol Chem 279:27502–27510
Banci L, Bertini I, Ciofi-Baffoni S, Kandias NG, Robinson NJ, Spyroulias GA, Su XC, Tottey S, Vanarotti M (2006) Proc Natl Acad Sci USA 103:8320–8325
Vothknecht UC, Westhoff P (2001) Biochim Biophys Acta 1541:91–101
Durán RV, Hervás M, De La Rosa MA, Navarro JA (2004) J Biol Chem 279:7229–7233
Tottey S, Rich PR, Rondet SAM, Robinson NJ (2001) J Biol Chem 276:9999–20004
Tottey S, Rondet SAM, Borrelly GPM, Robinson PJ, Rich PR, Robinson NJ (2002) J Biol Chem 277:5490–5497
Banci L, Bertini I, Ciofi-Baffoni S, Del Conte R, Gonnelli L (2003) Biochemistry 42:1939–1949
Arnesano F, Banci L, Bertini I, Cantini F, Ciofi-Baffoni S, Huffman DL, O’Halloran TV (2001) J Biol Chem 276:41365–41376
Banci L, Bertini I, Cantini F, Felli IC, Gonnelli L, Hadjiliadis N, Pierattelli R, Rosato A, Voulgaris P (2006) Nat Chem Biol 2:367–368
Arnesano F, Banci L, Bertini I, Bonvin AM (2004) Structure 12:669–676
González-Guerrero M, Argüello JM (2008) Proc Natl Acad Sci USA 105:5992–5997
Borrelly GP, Rondet SA, Tottey S, Robinson NJ (2004) Mol Microbiol 53:217–227
Thelwell C, Robinson NJ, Turner-Cavet JS (1998) Proc Natl Acad Sci USA 85:10728–10733
Pennella MA, Shokes JE, Cosper NJ, Scott RA, Giedroc DP (2003) Proc Natl Acad Sci USA 100:3713–3718
Harvie DR, Andreini C, Cavallaro G, Meng W, Connolly BA, Yoshida K, Fujita Y, Harwood CR, Radford DS, Tottey S, Cavet JS, Robinson NJ (2006) Mol Microbiol 59:1341–1356
Liu T, Chen X, Ma Z, Shokes J, Hemmingsen L, Scott RA, Giedroc DP (2008) Biochemistry 47:10564–10575
Huckle JW, Morby AP, Turner JS, Robinson NJ (1993) Mol Microbiol 7:177–187
Morby AP, Turner JS, Huckle JW, Robinson NJ (1993) Nucleic Acids Res 21:921–925
Tottey S, Waldron KJ, Firbank SJ, Reale B, Bessant C, Sato K, Cheek TR, Gray J, Banfield MJ, Dennison C, Robinson NJ (2008) Nature 455:1138–1142
Sokołowska M, Bal W (2005) J Inorg Biochem 99:1653–1660
Xiao Z, Loughlin F, George GN, Howlett GJ, Wedd AG (2004) J Am Chem Soc 126:3081–3090
Hunt JB, Neece SH, Ginsburg A (1985) Anal Biochem 146:150–157
Busenlehner LS, Weng TC, Penner-Hahn JE, Giedroc DP (2002) J Mol Biol 319:685–701
Campbell DR, Chapman KE, Waldron KJ, Tottey S, Kendall S, Cavallaro G, Andreini C, Hinds J, Stoker NG, Robinson NJ, Cavet JS (2007) J Biol Chem 282:32298–32310
Banci L, Bertini I, Ciofi-Baffoni S, Poggi L, Vanarotti M, Tottey S, Waldron KJ, Robinson NJ (2009) J Biol Inorg Chem (in press)
Sazinsky MH, LeMoine B, Orofino M, Davydov R, Bencze KZ, Stemmler TL, Hoffman BM, Argüello JM, Rosenzweig AC (2007) J Biol Chem 282:25950–25959
Portmann R, Magnani D, Stoyanov JV, Schmechel A, Multhaup G, Solioz M (2004) J Biol Inorg Chem 9:396–402
Cobine P, Wickramasinghe WA, Harrison MD, Weber T, Solioz M, Dameron CT (1999) FEBS Lett 445:27–30
Leary SC, Sasarman F, Nishimura T, Shoubridge EA (2009) Hum Mol Genet (in press)
Banci L, Bertini I, Ciofi-Baffoni S, Hadjiloi T, Martinelli M, Palumaa P (2008) Proc Natl Acad Sci USA 105:6803–6808
Abriata LA, Banci L, Bertini I, Ciofi-Baffoni S, Gkazonis P, Spyroulias GA, Vila AJ, Wang S (2008) Nat Chem Biol 4:599–601
Furukawa Y, Torres AS, O’Halloran TV (2004) EMBO J 23:2872–2881