In Vitro Cellular & Developmental Biology - Plant

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Somatic embryogenesis from the thin cell layer cultures of zygotic and somatic embryos of Aesculus hippocastanum
In Vitro Cellular & Developmental Biology - Plant - - Trang 1-8 - 2023
Phisouda Sivilay, Yong Eui Choi
The thin cell layers (TCLs) of zygotic embryos of Aesculus hippocastanum were cultured on Murashige and Skoog (MS) medium with and without 2,4-D. Somatic embryos developed directly on the TCLs of zygotic embryos at the highest frequency on hormone-free medium. The frequency of direct somatic embryo formation was suppressed by 2,4-D treatment. Microscopic observation revealed that compact and nodul...... hiện toàn bộ
Rapid production of transgenic sugarcane with the introduction of simple loci following biolistic transfer of a minimal expression cassette and direct embryogenesis
In Vitro Cellular & Developmental Biology - Plant - Tập 48 - Trang 15-22 - 2011
Yogesh Taparia, Walid M. Fouad, Maria Gallo, Fredy Altpeter
A protocol is described that supports the production of transgenic sugarcane plants ready for transfer to soil within 3 mo from culture initiation. Biolistic gene transfer into cross-sections of immature leaf whorl explants followed by direct somatic embryogenesis resulted in the stable genetic transformation of the commercially important sugarcane cultivar CP 88-1762. Accelerating the production ...... hiện toàn bộ
2017 In Vitro Biology Meeting, June 10–14, Raleigh, North Carolina
In Vitro Cellular & Developmental Biology - Plant - Tập 53 Số 6 - Trang 641-665 - 2017
Regulation of the synthesis of human chorionic gonadotropin by strains of HeLa cells in culture
In Vitro Cellular & Developmental Biology - Plant - Tập 14 - Trang 775-778 - 1978
Janice Yang Chou
Thirty-seven strains of HeLa cells were examined for their ability to synthesize human chorionic gonadotropin (hCG) and its alpha subunit (hCG-α) in culture. Synthesis of hCG-α and hCG also was investigated in the presence of sodium butyrate and 5-bromo-2′-deoxyuridine (BrdUrd). All HeLa strains synthesized hCG-α in culture. Sodium butyrate increased the synthesis of hCG-α in all HeLa cells; BrdUr...... hiện toàn bộ
Biotechnological efforts for preserving and enhancing temperate hardwood tree biodiversity, health, and productivity
In Vitro Cellular & Developmental Biology - Plant - Tập 47 - Trang 123-147 - 2010
Paula M. Pijut, Shaneka S. Lawson, Charles H. Michler
Hardwood tree species in forest, plantation, and urban environments (temperate regions of the world) are important biological resources that play a significant role in the economy and the ecology of terrestrial ecosystems, and they have aesthetic and spiritual value. Because of these many values of hardwood tree species, preserving forest tree biodiversity through the use of biotechnological appro...... hiện toàn bộ
Porcine aortic organ culture: A model to study the cellular response to vascular injury
In Vitro Cellular & Developmental Biology - Plant - Tập 20 Số 7 - Trang 535-542 - 1984
Gotlieb, Avrum I., Boden, Patricia
Organ cultures of porcine thoracic aorta were studied to define the characteristics of this system as a model to study the reaction of endothelial cells (ECs) and the underlying smooth muscle cells (SMCs) to injury. Both nonwounded and wounded cultures the latter having had part of the endothelial surface gently denuded with a scalpel blade, were studied over a 7 d period by scanning and transmiss...... hiện toàn bộ
Betalain producing cell cultures ofBeta vulgaris L. var. bikores monogerm (red beet)
In Vitro Cellular & Developmental Biology - Plant - Tập 28 - Trang 39-45 - 1992
Roland R. Leathers, Catherine Davin, Jean Pierre Zrÿd
The betalains are a class of natural pigments comprising the yellow betaxanthins and the violet betacyanins. Callus lines developed fromBeta vulgaris, L. var. bikores monogerm exhibited cell colors ranging from white/green (nonpigmented) through yellow, orange, red, and violet and were representative of all betalain pigments found in the whole plant. The betalains have gained particular interest f...... hiện toàn bộ
Effect of activated charcoal on multiplication of African yam (Dioscorea cayenensis-rotundata) nodal segments using a temporary immersion bioreactor (RITA®)
In Vitro Cellular & Developmental Biology - Plant - Tập 50 - Trang 210-216 - 2013
Frederik Polzin, Isabelle Sylvestre, Eveline Déchamp, Pascal Ilbert, Hervé Etienne, Florent Engelmann
In this study, we compared the growth of Dioscorea cayenensis-rotundata (African yam) nodal segments, using semisolid medium in test tubes and liquid medium in 1-L Recipient for Automated Temporary Immersion (RITA®) temporary immersion bioreactors (TIB), and the application of various culture parameters. The addition of activated charcoal (AC) had a positive effect on the growth of nodal segments,...... hiện toàn bộ
An efficient method for in vitro regeneration of leafy spurge (Euphorbia esula L.)
In Vitro Cellular & Developmental Biology - Plant - Tập 44 - Trang 548-556 - 2008
Bin Xu, Wenhao Dai, Wun S. Chao
Leafy spurge (Euphorbia esula L.) is a perennial, invasive weed used as a model to study invasive plant behavior, because molecular tools (such as a deep expressed sequence tag database and deoxyribonucleic acid microarrays) have been developed for this species. However, the lack of effective in vitro regeneration and genetic transformation systems has hampered molecular approaches to study leafy ...... hiện toàn bộ
Kinetic models of hemopoietic stem cell populations
In Vitro Cellular & Developmental Biology - Plant - Tập 4 Số 1 - Trang 14-21 - 1969
L. G. Lajtha
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