In Vitro Cellular & Developmental Biology - Plant
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Theme 8- Public Understanding of Science
In Vitro Cellular & Developmental Biology - Plant - Tập 54 - Trang 128-132 - 2018
Pluronic F-68: an answer for shoot regeneration recalcitrance in microspore-derived Brassica napus embryos
In Vitro Cellular & Developmental Biology - Plant - Tập 47 - Trang 282-288 - 2011
Recalcitrance to tissue culture is observed in some genotypes of Brassica napus. Several studies have confirmed that Pluronic F-68 has growth-promoting effects on numerous tissue types. This work investigated the effect of the non-ionic surfactant Pluronic F-68 at four concentrations (0.1%, 0.25%, 0.5%, and 1% (w/v)) on the responsiveness of recalcitrant B. napus lines to tissue culture. Microspor...... hiện toàn bộ
Presence of insulinlike growth factor receptors and lack of insulin receptors on fetal bovine smooth muscle cells
In Vitro Cellular & Developmental Biology - Plant - Tập 24 - Trang 921-926 - 1988
Previous investigations have demonstrated specific receptors and associated mitogenic actions for insulin and insulinlike growth factors I and II (IGF-I and II) in postnatal bovine aortic smooth muscle. Using fetal tissue we have observed different patterns of binding and action for these peptides. Smooth muscle cells isolated from near-term fetal bovine aortae were studied in early passage. Speci...... hiện toàn bộ
Endophytic bacteria from Ilex paraguariensis shoot cultures: localization, characterization, and response to isothiazolone biocides
In Vitro Cellular & Developmental Biology - Plant - Tập 49 - Trang 326-332 - 2013
Nodal segment explants of Ilex paraguariensis, collected from greenhouse-grown plants, were found to contain endophytic bacteria. After culturing in bioreactors, 16 rRNA gene analyses and analytical profile index biochemical tests were used to identify these bacteria as Stenotrophomonas malthophilia. The presence of bacterial cells in the intercellular spaces of stem cortical parenchyma was detect...... hiện toàn bộ
Production of haploid and doubled haploid plants of Allium tuncelianum (Kollman) Özhatay, Matthew and Şiraneci via in vitro gynogenesis
In Vitro Cellular & Developmental Biology - Plant - Tập 59 - Trang 700-710 - 2023
Allium tuncelianum (Kollman) Özhatay, Matthew and Şiraneci forms a single-cloved edible white bulb with a mild garlic (A. sativum) odor and taste. Its ability to form seeds makes it suitable for genetic improvement via classical and modern approaches. A detailed study was carried out to determine the gynogenic and somatic plant regeneration potential of two A. tuncelianum accessions (AT1 and AT2)....... hiện toàn bộ
A cryopreservation protocol for ex situ conservation of terrestrial orchids using asymbiotic primary and secondary (adventitious) protocorms
In Vitro Cellular & Developmental Biology - Plant - - 2015
In a bid to better conserve endangered terrestrial orchids, we detail cryogenic research using a widely distributed terrestrial orchid, Caladenia latifolia, as a model species for development of cryopreservation for primary (seed generated) and secondary (adventitious) protocorms. Primary protocorm cryopreservation (using droplet vitrification) involved a number of experimental lines of inquiry: i...... hiện toàn bộ
Loss of growth hormone-dependent characteristics of rat hepatocytes in culture
In Vitro Cellular & Developmental Biology - Plant - Tập 23 - Trang 303-307 - 1987
The liver of rodents is sexually differentiated, i.e. the female liver differs from the male liver. This differentiation is largely controlled by the pattern of growth hormone (GH) secretion. We have attempted to maintain GH-dependent differentiation of cultured rat hepatocytes. We examined the level of alcohol dehydrogenase (ADH) activity, which responds to GH and is higher in female than in male...... hiện toàn bộ
Co-expression of the tobacco anthranilate synthase β subunit with its feedback-insensitive α subunit as a selectable marker that also markedly increases the free tryptophan content
In Vitro Cellular & Developmental Biology - Plant - Tập 51 - Trang 564-570 - 2015
Anthranilate synthase (AS), a tetramer consisting of two α subunits and two β subunits, is the key control enzyme in the tryptophan (Trp) biosynthesis pathway. A naturally occurring α subunit of AS called ASA2 that is insensitive to Trp feedback inhibition was isolated from a tobacco suspension cell culture and has been extensively studied and used for both nuclear and plastid transformation. Howe...... hiện toàn bộ
Alternative statistical analyses for micropropagation: A practical case of proliferation and rooting phases in Viburnum opulus
In Vitro Cellular & Developmental Biology - Plant - Tập 39 - Trang 429-436 - 2003
The kinds of data obtained in micropropagation studies are very often problematic, since they do not follow continous distribution and observations through culture vessels complicate measurement. Accordingly, standard analyses are often used, leading to misinterpretation of results. In this paper, we present a study of Viburnum opulus micropropagation using planned contrasts and fitting regression...... hiện toàn bộ
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