Gelrite as an alternative to agar for micropropagation and microtuberization of Solanum tuberosum L. cv. BarakaIn Vitro Cellular & Developmental Biology - Plant - Tập 33 - Trang 195-199 - 1997
J. Veramendi, M. J. Villafranca, V. Sota, A. M. Mingo-Castel
Phytagel™ allowed the production of longer internodes, faster in vitro tuberization, and larger tubers in Solanum tuberosum L. cv. Baraka as compared to Difco Bacto-agar during both an 8-h photoperiod or in darkness. It also allowed a higher tuberization percentage in the dark. Only a 0.2% (wt/vol) Phytagel allowed optimal micropropagation and microtuberization under the photoperiod regime used. W...... hiện toàn bộ
Genetic transformation of Linum by particle bombardmentIn Vitro Cellular & Developmental Biology - Plant - Tập 35 - Trang 456-465 - 1999
Teguh Wijayanto, Alan McHughen
Linseed flax (Linum usitatissimum L.) was transformed by bombarding hypocotyl tissues with gold particles coated with plasmid DNA carrying the β-glucuronidase (GUS) (uid-A) and neomycin phosphotransferase II (npt-II) genes. Transient expression of the introduced β-glucuronidase gene was used to study factors influencing the DNA delivery, while progeny analyses confirmed stable transformation. The ...... hiện toàn bộ
An alternative method for the isolation of NS-1 hybridomas using cholesterol auxotrophy of NS-1 mouse myeloma cellsIn Vitro Cellular & Developmental Biology - Plant - Tập 25 - Trang 477-480 - 1989
Yoshinari Myoken, Tetsuji Okamoto, Teruhiko Osaki, Masafumi Yabumoto, Gordon H. Sato, Kazuaki Takada, J. Denry Sato
We have used the cholesterol auxotrophy of NS-1 mouse myeloma cells as the basis for selecting NS-1 hybridomas. The outgrowth of nascent NS-1 hybridomas in cholesterol-free serum-free medium was 3- to 9-fold more efficient than that in HAT medium and resulted in 3- to 13-times as many antigen-reactive hybridoma wells. This method of hybridoma selection can be applied with any sterol-dependent pare...... hiện toàn bộ
Rapid clonal propagation of Curculigo orchioides Gaertn., an endangered medicinal plantIn Vitro Cellular & Developmental Biology - Plant - Tập 43 - Trang 140-143 - 2007
Salema Valencio Francis, Sunil Kumar Senapati, Gyana Ranjan Rout
An efficient protocol was developed for in vitro clonal propagation of Curculigo orchioides Gaertn. through apical meristem culture. Multiple shoots were induced from apical meristems grown on Murashige and Skoog (MS) basal medium supplemented with 1.5 mg l−1 6-benzyladenine (BA), 100 mg l−1 adenine sulfate (Ads) and 3% sucrose. Inclusion of indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA...... hiện toàn bộ
Shoot apical meristem: A sustainable explant for genetic transformation of cereal cropsIn Vitro Cellular & Developmental Biology - Plant - Tập 41 - Trang 187-200 - 2005
Mariam B. Sticklen, Hesham F. Oraby
Immature zygotic embryo has been the widely used explant source to develop embryogenic callus lines, cell suspensions and protoplasts for transformation of cereal crops including maize, wheat, rice, oat, barley, sorghum, and millet. However, the lack of competence of immature embryos in certain elite lines is still a barrier to rontine production of transgenic cereal crops in certain commercial cu...... hiện toàn bộ
Somatic embryogenesis from the thin cell layer cultures of zygotic and somatic embryos of Aesculus hippocastanumIn Vitro Cellular & Developmental Biology - Plant - - Trang 1-8 - 2023
Phisouda Sivilay, Yong Eui Choi
The thin cell layers (TCLs) of zygotic embryos of Aesculus hippocastanum were cultured on Murashige and Skoog (MS) medium with and without 2,4-D. Somatic embryos developed directly on the TCLs of zygotic embryos at the highest frequency on hormone-free medium. The frequency of direct somatic embryo formation was suppressed by 2,4-D treatment. Microscopic observation revealed that compact and nodul...... hiện toàn bộ