In Vitro Cellular & Developmental Biology - Plant

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Gelrite as an alternative to agar for micropropagation and microtuberization of Solanum tuberosum L. cv. Baraka
In Vitro Cellular & Developmental Biology - Plant - Tập 33 - Trang 195-199 - 1997
J. Veramendi, M. J. Villafranca, V. Sota, A. M. Mingo-Castel
Phytagel™ allowed the production of longer internodes, faster in vitro tuberization, and larger tubers in Solanum tuberosum L. cv. Baraka as compared to Difco Bacto-agar during both an 8-h photoperiod or in darkness. It also allowed a higher tuberization percentage in the dark. Only a 0.2% (wt/vol) Phytagel allowed optimal micropropagation and microtuberization under the photoperiod regime used. W... hiện toàn bộ
Phytohemagglutinin-enhanced hybrid colony formation by cells from aged mice: Expression of th 4mod-1 mouse locus in human-mouse hybrids
In Vitro Cellular & Developmental Biology - Plant - Tập 23 - Trang 154-158 - 1987
Patricia G. West, Wilber W. Baker
Cells from a continuous human line and freshly isolated cells from old adult mice heterozygous at theMod-1 locus were fused in the presence of polyethylene glycol (PEG). The production of hybrid cells, as a function of PEG concentration in the presence and absence of phytohemagglutining (PHA), was measured by cell survival and proliferation on selective medium. The incorporation of PHA into the fu... hiện toàn bộ
Genetic transformation of Linum by particle bombardment
In Vitro Cellular & Developmental Biology - Plant - Tập 35 - Trang 456-465 - 1999
Teguh Wijayanto, Alan McHughen
Linseed flax (Linum usitatissimum L.) was transformed by bombarding hypocotyl tissues with gold particles coated with plasmid DNA carrying the β-glucuronidase (GUS) (uid-A) and neomycin phosphotransferase II (npt-II) genes. Transient expression of the introduced β-glucuronidase gene was used to study factors influencing the DNA delivery, while progeny analyses confirmed stable transformation. The ... hiện toàn bộ
An alternative method for the isolation of NS-1 hybridomas using cholesterol auxotrophy of NS-1 mouse myeloma cells
In Vitro Cellular & Developmental Biology - Plant - Tập 25 - Trang 477-480 - 1989
Yoshinari Myoken, Tetsuji Okamoto, Teruhiko Osaki, Masafumi Yabumoto, Gordon H. Sato, Kazuaki Takada, J. Denry Sato
We have used the cholesterol auxotrophy of NS-1 mouse myeloma cells as the basis for selecting NS-1 hybridomas. The outgrowth of nascent NS-1 hybridomas in cholesterol-free serum-free medium was 3- to 9-fold more efficient than that in HAT medium and resulted in 3- to 13-times as many antigen-reactive hybridoma wells. This method of hybridoma selection can be applied with any sterol-dependent pare... hiện toàn bộ
Heritable DNA-free genome editing of canola (Brassica napus L.) using PEG-mediated transfection of isolated protoplasts
In Vitro Cellular & Developmental Biology - Plant - Tập 58 Số 3 - Trang 447-456 - 2022
V. A. Sidorov, Dafu Wang, É. Nagy, Charles Armstrong, Steven R. H. Beach, Yuanji Zhang, Jeanna Groat, Sam Yang, Peizhen Yang, Larry A. Gilbertson
Rapid clonal propagation of Curculigo orchioides Gaertn., an endangered medicinal plant
In Vitro Cellular & Developmental Biology - Plant - Tập 43 - Trang 140-143 - 2007
Salema Valencio Francis, Sunil Kumar Senapati, Gyana Ranjan Rout
An efficient protocol was developed for in vitro clonal propagation of Curculigo orchioides Gaertn. through apical meristem culture. Multiple shoots were induced from apical meristems grown on Murashige and Skoog (MS) basal medium supplemented with 1.5 mg l−1 6-benzyladenine (BA), 100 mg l−1 adenine sulfate (Ads) and 3% sucrose. Inclusion of indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA... hiện toàn bộ
Interactive effects of nitrate and phosphate salts, sucrose, and inoculum culture age on growth and sesquiterpene production inArtemisia annua hairy root cultures
In Vitro Cellular & Developmental Biology - Plant - - 1998
P. J. Weathers, D. D. Hemmavanh, D. B. Walcerz, R. D. Cheetham, T. C. Smith
Somatic generation of immune diversity
In Vitro Cellular & Developmental Biology - Plant - Tập 24 - Trang 253-265 - 1988
Susumu Tonegawa
Shoot apical meristem: A sustainable explant for genetic transformation of cereal crops
In Vitro Cellular & Developmental Biology - Plant - Tập 41 - Trang 187-200 - 2005
Mariam B. Sticklen, Hesham F. Oraby
Immature zygotic embryo has been the widely used explant source to develop embryogenic callus lines, cell suspensions and protoplasts for transformation of cereal crops including maize, wheat, rice, oat, barley, sorghum, and millet. However, the lack of competence of immature embryos in certain elite lines is still a barrier to rontine production of transgenic cereal crops in certain commercial cu... hiện toàn bộ
Somatic embryogenesis from the thin cell layer cultures of zygotic and somatic embryos of Aesculus hippocastanum
In Vitro Cellular & Developmental Biology - Plant - - Trang 1-8 - 2023
Phisouda Sivilay, Yong Eui Choi
The thin cell layers (TCLs) of zygotic embryos of Aesculus hippocastanum were cultured on Murashige and Skoog (MS) medium with and without 2,4-D. Somatic embryos developed directly on the TCLs of zygotic embryos at the highest frequency on hormone-free medium. The frequency of direct somatic embryo formation was suppressed by 2,4-D treatment. Microscopic observation revealed that compact and nodul... hiện toàn bộ
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