In Vitro Cellular & Developmental Biology - Plant
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Somatic embryogenesis from the thin cell layer cultures of zygotic and somatic embryos of Aesculus hippocastanum
In Vitro Cellular & Developmental Biology - Plant - - Trang 1-8 - 2023
The thin cell layers (TCLs) of zygotic embryos of Aesculus hippocastanum were cultured on Murashige and Skoog (MS) medium with and without 2,4-D. Somatic embryos developed directly on the TCLs of zygotic embryos at the highest frequency on hormone-free medium. The frequency of direct somatic embryo formation was suppressed by 2,4-D treatment. Microscopic observation revealed that compact and nodul......
Rapid production of transgenic sugarcane with the introduction of simple loci following biolistic transfer of a minimal expression cassette and direct embryogenesis
In Vitro Cellular & Developmental Biology - Plant - Tập 48 - Trang 15-22 - 2011
A protocol is described that supports the production of transgenic sugarcane plants ready for transfer to soil within 3 mo from culture initiation. Biolistic gene transfer into cross-sections of immature leaf whorl explants followed by direct somatic embryogenesis resulted in the stable genetic transformation of the commercially important sugarcane cultivar CP 88-1762. Accelerating the production ......
2017 In Vitro Biology Meeting, June 10–14, Raleigh, North Carolina
In Vitro Cellular & Developmental Biology - Plant - Tập 53 Số 6 - Trang 641-665 - 2017
Biotechnological efforts for preserving and enhancing temperate hardwood tree biodiversity, health, and productivity
In Vitro Cellular & Developmental Biology - Plant - Tập 47 - Trang 123-147 - 2010
Hardwood tree species in forest, plantation, and urban environments (temperate regions of the world) are important biological resources that play a significant role in the economy and the ecology of terrestrial ecosystems, and they have aesthetic and spiritual value. Because of these many values of hardwood tree species, preserving forest tree biodiversity through the use of biotechnological appro......
Porcine aortic organ culture: A model to study the cellular response to vascular injury
In Vitro Cellular & Developmental Biology - Plant - Tập 20 Số 7 - Trang 535-542 - 1984
Organ cultures of porcine thoracic aorta were studied to define the characteristics of this system as a model to study the reaction of endothelial cells (ECs) and the underlying smooth muscle cells (SMCs) to injury. Both nonwounded and wounded cultures the latter having had part of the endothelial surface gently denuded with a scalpel blade, were studied over a 7 d period by scanning and transmiss......
Effect of activated charcoal on multiplication of African yam (Dioscorea cayenensis-rotundata) nodal segments using a temporary immersion bioreactor (RITA®)
In Vitro Cellular & Developmental Biology - Plant - Tập 50 - Trang 210-216 - 2013
In this study, we compared the growth of Dioscorea cayenensis-rotundata (African yam) nodal segments, using semisolid medium in test tubes and liquid medium in 1-L Recipient for Automated Temporary Immersion (RITA®) temporary immersion bioreactors (TIB), and the application of various culture parameters. The addition of activated charcoal (AC) had a positive effect on the growth of nodal segments,......
An efficient method for in vitro regeneration of leafy spurge (Euphorbia esula L.)
In Vitro Cellular & Developmental Biology - Plant - Tập 44 - Trang 548-556 - 2008
Leafy spurge (Euphorbia esula L.) is a perennial, invasive weed used as a model to study invasive plant behavior, because molecular tools (such as a deep expressed sequence tag database and deoxyribonucleic acid microarrays) have been developed for this species. However, the lack of effective in vitro regeneration and genetic transformation systems has hampered molecular approaches to study leafy ......
Amino acid utilization by L-M strain mouse cells in a chemically defined medium
In Vitro Cellular & Developmental Biology - Plant - Tập 7 - Trang 330-343 - 1972
The amino acid requirements of strain L-M mouse cells grown in a chemically defined medium (2×Eagle) containing only the 13 essential amino acids (EAA) were investigated. Medium and acid hydrolysate samples were analyzed for amino acid content by the method of ion exchange chromatography. The extent of utilization of the EAA differed;e.g. after 120 hr of cell growth without medium change, glutamin......
Quality control studies on fetal bovine serum used in tissue culture
In Vitro Cellular & Developmental Biology - Plant - Tập 7 - Trang 174-189 - 1971
The quality of the fetal bovine serum (FBS) produced for tissue culture purposes by eight commercial suppliers in the United States was tested over a period of 1 year. The results were compared with tests on some special FBS produced during the same period under conditions which included maximal sterile precautions, freedom from whole cells, and rapid processing in the cold. The findings were that......
Effects of proliferation, maturation, and desiccation methods on conversion of soybean somatic embryos
In Vitro Cellular & Developmental Biology - Plant - Tập 39 - Trang 623-628 - 2003
Cermination of soybean [Glycine max (L.) Merrill] somatic embryos and conversion to whole plants are generally low. This study was conducted to investigate the effects of proliferation, maturation, and desiccation methods on conversion of soybean somatic embryos to plants. Soybean cv. Jack somatic embryos, proliferated on a solid medium containing 90.5 μM (20 mgl−1) 2.4-dichlorophenoxyacetic acid ......
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