An alternative method for the isolation of NS-1 hybridomas using cholesterol auxotrophy of NS-1 mouse myeloma cells
Tóm tắt
We have used the cholesterol auxotrophy of NS-1 mouse myeloma cells as the basis for selecting NS-1 hybridomas. The outgrowth of nascent NS-1 hybridomas in cholesterol-free serum-free medium was 3- to 9-fold more efficient than that in HAT medium and resulted in 3- to 13-times as many antigen-reactive hybridoma wells. This method of hybridoma selection can be applied with any sterol-dependent parent cell line. Hybridomas established under serum-free culture conditions were growth inhibited by fetal calf serum.
Tài liệu tham khảo
Kohler, G.; Milstein, C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature (Lond.), 256:495–497; 1975.
Littlefield, J. W. Selection of hybrids from mating of fibroblasts in vitro and their presumed recombinants. Science 145:709–710; 1964.
Schnitzer, R. J.; Hawking, F. (Eds.) Experimental Chemotherapy, Vol. IV(1). Academic Press, New York; 1966.
Horenstein, A. L.; Glait, H. M.; Koss, A. An improved selection procedure for the rescue of hybridomas. A comparative study of methotrexate versus aminopterin. J. Immunol. Meth. 98:145–149; 1987.
Foung, S. K. H.; Sasaki, D. T.; Grumet, F. C.; Englemen, E. G. Production of functional human T-T hybridomas in selection medium lacking aminopterin and thymidine. Proc. Natl. Acad. Sci. USA 79:7484–7488; 1982.
Kawamoto, T.; Sato, J. D.; Le, A.; McClure, D. B.; Sato, G. H. Development of a serum-free medium for growth of NS-1 mouse myeloma cells and its application to the isolation of NS-1 hybridomas. Anal. Biochem. 130:445–453; 1983.
Sato, J. D.; Kawamoto, T.; McClure, D. B.; Sato, G. H. Cholesterol requirement of NS-1 mouse myeloma cells for growth in serum-free medium. Mol. Biol. Med. 2:121–134; 1984.
Chen, J.-K.; Okamoto, T.; Sato, J. D.; Sato, G. H.; McClure, D. B. Biochemical characterization of the cholesterol-dependent growth of the NS-1 mouse myeloma cell line. Exp. Cell. Res. 163:117–126; 1986.
Sato, J. D.; Welsh, C. J.; Cao, H.-T.; Okamoto, T.; Cabot, M. C. Cholesterol auxotrophy of NS-1 mouse myeloma cells: identification of an accumulated 3-ketosteroid precursor of cholesterol. (Abstract). FASEB J. 2:A579, 1988.
Sato, J. D.; Cao, H.-T.; Kayada, Y.; Cabot, M. C.; Sato, G. H.; Okamoto, T.; Welsh, C. J. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24:1223–1228; 1988.
Sato, J. D.; Kawamoto, T.; Okamoto, T. Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro. J. Exp. Med. 165:1761–1766; 1987.
Kohler, G.; Howe, S. C.; Milstein, C. Fusion between immunoglobulin secreting and nonsecreting myeloma cell lines. Eur. J. Immunol. 6:292–295; 1976.
Sato, J. D.; Kawamoto, T.; Le, A.; Mendelsohn, J.; Polikoff, J.; Sato, G. H. Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors. Mol. Biol. Med. 1:511–529; 1983.
Hunter, W. M.; Greenwood, F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature (Lond.) 194:495–496; 1962.
Bonino, F.; Recchia, S.; Ponzetto, A.; Filippone, B.; Palla, M.; Zanetti, A. R.; Ferroni, P. A solid-phase enzyme immunoassay (EIA) for detection of HBeAg and anti-HBe. J. Immunol. Methods 33:195–200; 1980.
Loo, D. T.; Fuquacy, J. I.; Rawson, C. L.; Barnes, D. W. Extended culture of mouse embryo cells without senescence: inhibition of serum. Science 236:200–202; 1987.