Rapid clonal propagation of Curculigo orchioides Gaertn., an endangered medicinal plant
Tóm tắt
An efficient protocol was developed for in vitro clonal propagation of Curculigo orchioides Gaertn. through apical meristem culture. Multiple shoots were induced from apical meristems grown on Murashige and Skoog (MS) basal medium supplemented with 1.5 mg l−1 6-benzyladenine (BA), 100 mg l−1 adenine sulfate (Ads) and 3% sucrose. Inclusion of indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) in the culture medium improved the formation of multiple shoots. The highest frequency of multiplication was obtained on MS medium supplemented with 1.5 mg l−1 BA, 100 mg l−1 Ads, 0.25 mg l−1 IBA and 3% sucrose. Rooting was achieved upon transferring the micro-shoots to half-strength MS medium containing 0.25 mg l−1 IBA and 2% sucrose. Micropropagtated plantlets were hardened in the greenhouse and successfully established in soil.
Tài liệu tham khảo
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