Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumorsGenes Chromosomes and Cancer - Tập 10 Số 4 - Trang 231-243 - 1994
Olli Kallioniemi, Anne Kallioniemi, Jim Piper, Jorma Isola, F M Waldman, Joe W. Gray, Dan Pinkel
AbstractComparative genomic hybridization (CGH) is a powerful new method for molecular cytogenetic analysis of cancer. In a single hybridization, CGH provides an overview of DNA sequence copy number changes (losses, deletions, gains, amplifications) in a tumor specimen and maps these changes on normal chromosomes. CGH is based on the in situ hybridization of differ...... hiện toàn bộ
Identification of amplified DNA sequences in breast cancer and their organization within homogeneously staining regionsGenes Chromosomes and Cancer - Tập 14 Số 3 - Trang 155-163 - 1995
Martine Muleris, Anna Almeida, Michèle Gerbault‐Seureau, Bernard Malfoy, Bernard Dutrillaux
AbstractA modified comparative genomic hybridization (mCGH) technique was used to identify and map amplified DNA sequences in six homogeneously staining regions (hsr) from three primary breast carcinomas. Five different chromosomal regions and bands were identified as sites of amplification: 8p1, 17q21.1, 17q23 (two cases), 19q13.3, and 20q13.3. The mCGH site locat...... hiện toàn bộ
FGFRI and PLAT genes and DNA amplification at 8p 12 in breast and ovarian cancersGenes Chromosomes and Cancer - Tập 7 Số 4 - Trang 219-226 - 1993
Charles Theillet, José Adélaı̈de, Geneviève Louason, Françoise Bonnet‐Dorion, Jocelyne Jacquemier, Jalila Adnane, Michel Longy, Dionyssios Katsaros, P Sismondi, Patrick Gaudray, Daniel Birnbaum
AbstractSeveral chromosomal regions are found to be consistently amplified in human breast cancers. For two of these regions, 8p 12 and 10q26, we previously reported the amplification of genes encoding FGF receptors, FGFR1/FLG and FGFR2/BEK, in about 12% of breast tumors. The PLAT gene...... hiện toàn bộ
Chromosome analysis of 97 primary breast carcinomas: Identification of eight karyotypic subgroupsGenes Chromosomes and Cancer - Tập 12 Số 3 - Trang 173-185 - 1995
Nikos Pandis, Yuesheng Jin, Ludmila Gorunova, Catarina Petersson, Georgia Bardi, Ingrid Idvall, Björn Johansson, Christian Ingvar, Nils Mandahl, Felix Mitelman, S Heim
AbstractChromosome banding analysis of 97 short‐term cultured primary breast carcinomas revealed clonal aberrations in 79 tumors, whereas 18 were karyotypically normal. In 34 of the 79 tumors with abnormalities, two to eight clones per case were detected; unrelated clones were present in 27 (34%) cases, whereas only related clones were found in seven. These finding...... hiện toàn bộ
Allelic 3p Deletions in High‐Grade Carcinomas After Transformation In Vitro of Human Uroepithelial CellsGenes Chromosomes and Cancer - Tập 3 Số 5 - Trang 346-357 - 1991
Aloysius J. Klingelhutz, Shiqi Wu, E A Bookland, Catherine A. Reznikoff
AbstractRestriction fragment length polymorphism (RFLP) analysis for allelic losses on the chromosome arm 3p were performed on independent carcinomas produced in athymic nude mice after transformation in vitro of a pseudodiploid clonal SV40‐immortalized human uroepithelial cell line (SV‐HUC). We analyzed ten primary carcinomas with heterogeneous phenotypes for dele...... hiện toàn bộ
Identification of gains and losses of DNA sequences in primary bladder cancer by comparative genomic hybridizationGenes Chromosomes and Cancer - Tập 12 Số 3 - Trang 213-219 - 1995
Anne Kallioniemi, Olli Kallioniemi, Gil Citro, Guido Sauter, Sandy DeVries, Russell Kerschmann, Peter R. Caroll, F. Waldman
AbstractComparative genomic hybridization (CGH) makes it possible to detect losses and gains of DNA sequences along all chromosomes in a tumor specimen based on the hybridization of differentially labeled tumor and normal DNA to normal human metaphase chromosomes. In this study, CGH analysis was applied to the identification of genomic imbalances in 26 bladder canc...... hiện toàn bộ
Deletion mapping of chromosome II in carcinoma of the bladderGenes Chromosomes and Cancer - Tập 13 Số 1 - Trang 1-8 - 1995
Margaret E. Shaw, Margaret A. Knowles
AbstractDeletions of the short arm of chromosome II have been identified by both cytogenetic and molecular criteria in bladder and other types of solid tumor, indicating the presence of one or more suppressor loci in this region. To localize the II p deletion target(s) more precisely and to screen for loss of heterozygosity (LOH) on the long arm of the chromosome, ...... hiện toàn bộ
Losses of 3p, 11p, and 13q in ej/ras‐ transformable simian virus 40‐immortalized human uroepithelial cellsGenes Chromosomes and Cancer - Tập 4 Số 2 - Trang 158-168 - 1992
Chinghai Kao, Shiqi Wu, Manju Bhatthacharya, Lorraine F. Meisner, Catherine A. Reznikoff
AbstractFive independent clones of Simian virus 40 (SV40)‐immortalized human uroepithelial cells (CK/SV‐HUC) were established after transfection of HUC cultures from the same tissue donor with plasmids encoding SV40 large T and small t antigen genes. Each CK/SV‐HUC clone contained a unique SV40 integration site, and all expressed similar levels of SV40 mRNA. All fi...... hiện toàn bộ