Identification of amplified DNA sequences in breast cancer and their organization within homogeneously staining regions

Genes Chromosomes and Cancer - Tập 14 Số 3 - Trang 155-163 - 1995
Martine Muleris1, Anna Almeida1, Michèle Gerbault‐Seureau1, Bernard Malfoy1, Bernard Dutrillaux1
1Section de Recherche, Institut Curie, UMR 147 CNRS, Paris, France.

Tóm tắt

AbstractA modified comparative genomic hybridization (mCGH) technique was used to identify and map amplified DNA sequences in six homogeneously staining regions (hsr) from three primary breast carcinomas. Five different chromosomal regions and bands were identified as sites of amplification: 8p1, 17q21.1, 17q23 (two cases), 19q13.3, and 20q13.3. The mCGH site located on 17q21.1 was demonstrated to correspond to a 50–100‐fold amplification of ERBB2. Further in situ hybridization experiments were used to confirm the mCGH results and to characterize the organization of the amplified sequences within the hsr. In five of six instances, two or more chromosomal regions were found amplified in the same hsr. In the tumor with the less modified karyotype, the two hsr comprised DNA sequences from three different chromosomes and showed different patterns of amplification. In the tumor with the most rearranged karyotype, the hsr‐carrying chromosomes were formed by the translocation and amplification of sequences from three or four different chromosomal sites. This illustrates the complexity of the amplification process in breast cancers.

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Genes Chromosomes and Cancer

Tập 14 Số 3

155-163

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