Losses of 3p, 11p, and 13q in ej/ras‐ transformable simian virus 40‐immortalized human uroepithelial cells

Genes Chromosomes and Cancer - Tập 4 Số 2 - Trang 158-168 - 1992
Chinghai Kao1,2,3, Shiqi Wu2,3, Manju Bhatthacharya2,3, Lorraine F. Meisner2,3, Catherine A. Reznikoff2,3
1Departments of Biochemistry, University of Wisconsin, Madison, Wisconsin
2Departments of Comprehensive Cancer Center, University of Wisconsin, Madison, Wisconsin
3Departments of Human Oncology, University of Wisconsin, Madison, Wisconsin

Tóm tắt

AbstractFive independent clones of Simian virus 40 (SV40)‐immortalized human uroepithelial cells (CK/SV‐HUC) were established after transfection of HUC cultures from the same tissue donor with plasmids encoding SV40 large T and small t antigen genes. Each CK/SV‐HUC clone contained a unique SV40 integration site, and all expressed similar levels of SV40 mRNA. All five clones were nontumorigenic, but clones 2, 4, and 5 tumorigenically transformed after transfection at P19 with mutant EJ/ras and also spontaneously after 40 serial passages in vitro. In contrast, CK/SV‐HUC clones 1 and 3 did not transform when either approach was used. These differences in transformability among CK/SV‐HUC clones could not be predicted based on differences in SV40 gene expression nor on any in vitro growth property tested. In cytogenetic analyses, a transformable clone showed losses of three chromosome arms containing putative cancer suppressor gene regions, including 3p14→pter, 13q, and 11p, whereas the nontransformable clones showed none of these losses. Thus these data indicate that genetic losses on 3p, 11p, and 13q may contribute to tumorigenic transformation of SV40‐immortalized human uroepithelial cells.

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Genes Chromosomes and Cancer

Tập 4 Số 2

158-168

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