Detection of DNA amplification in 17 primary breast carcinomas with homogeneously staining regions by a modified comparative genomic hybridization technique

Genes Chromosomes and Cancer - Tập 10 Số 3 - Trang 160-170 - 1994
Martine Muleris1, Anna Almeida1, Michèle Gerbault‐Seureau1, Bernard Malfoy1, Bernard Dutrillaux1
1CNRS URA 620, Institut Curie, Section de Biologie, Paris, France

Tóm tắt

AbstractA modified comparative genomic hybridization (mCGH) technique was applied to a series of 17 primary breast carcinomas in which cytogenetic study (CG) demonstrated the presence of homogeneously staining region(s), suggesting the occurrence of DNA amplification. mCGH demonstrated recurrent amplifications of the whole chromosome arms 8q (9 times) and I q (7 times) and of DNA loci in the following bands: 11 q 13 (6 times), 9p 13 and 17q21.1 (4 times), I q21.1 and 16p 11.2 (3 times), and 8q22, 8q24.1, 10q22, 15q26, 17q23, and 20q 13.3 (twice). Amplification of whole chromosome arms is likely to have resulted from unbalanced translocations or isochromosomes, whereas amplifications of smaller chromosomal segments probably arose through real DNA amplification processes. In all tumors but one, more than one amplified locus was detected. The fact that many chromosomal sites were involved suggests that the process of amplification is complex and that many genes are potential targets. Genes Chromosom Cancer 10:160–170 (1994). © 1994 Wiley‐Liss, Inc.

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Genes Chromosomes and Cancer

Tập 10 Số 3

160-170

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