FEBS Letters
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Enhancement of the polycation‐mediated DNA uptake and cell transfection with Pluronic P85 block copolymer Polyelectrolyte complexes formed between DNA and poly(N ‐ethyl‐4‐vinylpyridinium) cations were shown to effectively transfect mammalian cells [7]. This work suggests that the polycation‐mediated uptake of the plasmid DNA and cell transfection are significantly enhanced when these complexes are administered simultaneously with a poly(ethylene oxide)block ‐poly(propylene oxide)‐block ‐poly(ethylene oxide) copolymer, Pluronic P85. The uptake studies were performed using radioactively labeled pRSV CAT plasmid on NIH 3T3, MDCK, and Jurkat cell lines. The transfection was investigated by chloramphenicol acetyltransferase assay using 3T3 cells as a model. The effects reported may be useful for the enhancement of the polycation‐mediated cell transfection.
FEBS Letters - Tập 389 Số 3 - Trang 278-280 - 1996
Mutants of <i>Streptococcus faecalis</i> concerning pyruvate dehydrogenation
FEBS Letters - Tập 64 - Trang 364-368 - 1976
The 12 kDa protein of potato virus M displays properties of a nucleic acid‐binding regulatory protein The 3' terminal 1.4 kb segment of potato virus M (PVM) genomic RNA was cloned and sequenced. This part of the viral genome encodes the capsid protein CP as well as a 12 kDa protein of as yet unknown function. Both proteins were expressed in bacteria and their nucleic acid‐binding properties studied. The 12 kDa protein (prl2), but not the capsid protein bound single‐ and double‐stranded nucleic acids. This property of prl2 in conjunction with a zinc finger motif located adjacent to a basic region of the 12 kDa protein suggests that it may act as a regulatory factor during virus replication.
FEBS Letters - Tập 276 - Trang 34-38 - 1990
Reconstitution of light‐harvesting chlorophyll‐protein complexes with Photosystem II complexes in soybean phosphatidylcholine liposomes Isolated Photosystem II (PS II) complexes and light‐harvesting chlorophyll‐protein complexes have been reconstituted, both separately and together, into soybean phosphatidylcholine liposomes. PS II activity, as measured by dichlorophenolindophenol reduction, increased by 70% following reconstitution of PS II complexes. Proteoliposomes containing both PS II and light‐harvesting chlorophyll‐protein complexes also exhibited enhanced quantum efficiencies at sub‐saturating light intensities. This indicates that the light‐harvesting complexes are interacting with the PS II complexes in proteoliposomes, to increase effectively their antenna size.
FEBS Letters - Tập 165 - Trang 151-155 - 1984
Enhanced IgG‐ and complement‐independent phagocytosis of sulfatide‐enriched human erythrocytes by human monocytes Phagocytosis by adherent human monocytes of human erythrocytes (RBC), sulfatide‐enriched by incubation with 10−12 to 10−9 M cerebroside sulfate, was enhanced approx. 6‐fold. Increased phagocytosis was observed only in RBC opsonized with fresh plasma, and not in non‐opsonized or serum‐opsonized RBC. Increased phagocytosis was immunoglobulin‐ and complement independent. Thrombospondin and von Willebrand factor, present in plasma but not in serum, and binding selectively to sulfatides, are likely mediators of the enhanced phagocytosis.
FEBS Letters - Tập 311 - Trang 67-70 - 1992
The interaction of 1‐anilino‐8‐naphthalene sulphonate with yeast alcohol dehydrogenase
FEBS Letters - Tập 15 - Trang 17-20 - 1971
Effect of 7β‐hydroxycholesterol on growth and membrane composition of <i>Mycoplasma capricolum</i> 7β‐OH cholesterol in a cholesterol rich growth medium (5–10 μg/ml) extended the lag period and slowed down the growth rate of Mycoplasma capricolum cells. In a cholesterol poor medium (0.5 μg/ml) inadequate to support growth, 7β‐OH cholesterol exerts a synergistic effect on growth. The 7β‐OH cholesterol was incorporated unchanged from the growth medium and could be recovered exclusively in the membrane fraction. The incorporation of the 7β‐OH cholesterol has no effect on the total phospholipid content but the DPG to PG ratio was markedly decreased. Exchange studies with lipid vesicles revealed that whereas most of the cholesterol underwent exchange, only about 20% of the 7β‐OH cholesterol was exchanged.
FEBS Letters - Tập 232 - Trang 354-358 - 1988
The irreversible photoreduction of a low potential component at low temperatures in a preparation of the green photosynthetic bacterium <i>Chlorobium thiosulphatophilum</i>
FEBS Letters - Tập 75 - Trang 33-36 - 1977
Synthesis of L‐[α‐<sup>15</sup>N]aminobutyric acid, L‐[<sup>15</sup>N]methionine and L‐[<sup>15</sup>N]valine by the use of beef heart glutamate dehydrogenase
FEBS Letters - Tập 75 - Trang 41-43 - 1977
Synthetic peptides as inactivators of multimeric enzymes: inhibition of <i>Plasmodium falciparum</i> triosephosphate isomerase by interface peptides Synthetic peptides corresponding to two distinct segments of the subunit interface of the homodimeric enzyme triosephosphate isomerase (residues 9–18, ANWKCNGTLE, peptide I; residues 68–79, KFGNGSYTGEVS, peptide II) from Plasmodium falciparum (PfTIM) have been investigated for their ability to act as inhibitors by interfering with the quaternary structure of the enzyme. An analog of peptide II containing cysteine at the site corresponding to position 74 and tyrosine at position 69 in the protein sequence KYGNGSCTGEVS (peptide III) was also investigated. A substantial fall in enzyme activity was observed following incubation of the enzyme with peptide II, whereas peptide I did not show any appreciable inhibition. The inhibitory effect was more pronounced on two mutants of PfTIM (Y74C and Y74G), both of which have reduced stability compared to the wild‐type protein due to an interface cavity. The IC50 value determined for peptide II is in the range of 0.6–0.8 μM. This study suggests that interface peptides of oligomeric enzymes can be used to inhibit dimeric enzymes by disrupting their native multimeric states and may provide lead structures for potential inhibitor design.
FEBS Letters - Tập 501 - Trang 19-23 - 2001
Tổng số: 24,185
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