Heat shock protein 70 inhibits the nuclear import of apoptosis‐inducing factor to avoid DNA fragmentation in TF‐1 cells during erythropoiesis

FEBS Letters - Tập 581 - Trang 109-117 - 2007
Julian Chun-Kin Lui1, Siu-Kai Kong1
1Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China

Tóm tắt

Loss of mitochondrial membrane potential (ΔΨm) and release of AIF (apoptosis‐inducing factor) from mitochondria are key steps in apoptosis. In TF‐1 model, ΔΨm was depolarized with AIF release during erythroid development. Yet, no DNA fragmentation was observed. When ΔΨm depolarization had been blocked, erythropoiesis was suppressed. Interestingly, heat shock protein 70 (Hsp70) was found transiently upregulated during depolarization and it retained AIF in the cytosol to avoid DNA damages. When Hsp inhibitor was added, DNA fragmentation occurred. We show this mechanism for the first time in erythropoiesis how cells with ΔΨm depolarization and AIF release escape apoptosis.

Tài liệu tham khảo

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FEBS Letters

Tập 581

109-117

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