Engineering in Life Sciences
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Analysis of intracellular metabolites of Corynebacterium glutamicum at high cell density with automated sampling and filtration and assessment of engineered enzymes for effective l ‐lysine production
Engineering in Life Sciences - Tập 17 Số 5 - Trang 512-522 - 2017
Engineering of enzymes and pathways is generally required for the development of
efficient strains for bioproduction processes. To this end, quantitative and
reliable data of intracellular metabolites are highly desired, but often not
available, especially for conditions more close to industrial applications, i.e.
at high cell density and product concentration. Here, we investigated the
intracellu...
Automated fast filtration and on‐filter quenching improve the intracellular metabolite analysis of microorganisms
Engineering in Life Sciences - Tập 14 Số 2 - Trang 135-142 - 2014
To reliably determine intracellular metabolite concentrations in microorganisms,
accurate sampling and sample inactivation strategies are crucial. Here, we
present a method for automated fast filtration and on‐filter quenching of
microbial samples to overcome metabolite leakage induced by cold shock and
significantly reduce the sampling and treatment time compared to manual
filtration methods. The...
Improved electrocompetence and metabolic engineering of Clostridium pasteurianum reveals a new regulation pattern of glycerol fermentation
Engineering in Life Sciences - Tập 19 Số 6 - Trang 412-422 - 2019
AbstractClostridium pasteurianum produces industrially valuable chemicals such
as n‐butanol and 1,3‐propanediol from fermentations of glycerol and glucose.
Metabolic engineering for increased yields of selective compounds is not well
established in this microorganism. In order to study carbon fluxes and to
selectively increase butanol yields, we integrated the latest advances in genome
editing to ...
Metabolomic and kinetic investigations on the electricity‐aided production of butanol by Clostridium pasteurianum strains
Engineering in Life Sciences - Tập 21 Số 3-4 - Trang 181-195 - 2021
AbstractIn this contribution, we studied the effect of electro‐fermentation on
the butanol production of Clostridium pasteurianum strains by a targeted
metabolomics approach. Two strains were examined: an electrocompetent wild type
strain (R525) and a mutant strain (dhaB mutant) lacking formation of
1,3‐propanediol (PDO). The dhaB‐negative strain was able to grow on glycerol
without formation of P...
A novel All‐in‐One electrolysis electrode and bioreactor enable better study of electrochemical effects and electricity‐aided bioprocesses
Engineering in Life Sciences - Tập 18 Số 8 - Trang 600-610 - 2018
AbstractAn autoclavable All‐in‐One electrolysis electrode in a rod shape
assembly is developed as a new tool for bioelectrochemical systems and
electricity‐aided bioprocesses. It can replace the classic two‐chamber
bioelectrochemical system for electrolysis reactions, be inserted into
conventional bioreactors and is easily adaptable as electrocatalytic surface or
generator of super‐fine bubbles (H...
Effects of anaerobic digestion on digestate nutrient availability and crop growth: A review
Engineering in Life Sciences - Tập 12 Số 3 - Trang 242-257 - 2012
Anaerobic digestion (AD) for biogas production leads to several changes in the
composition of the resulting digestates compared to the original feedstock
(ammonia content, pH, carbon to nitrogen ratio, etc.), which are relevant for
the plant availability of macro‐ and micronutrients after field application.
Increased NH4+‐N content in digested slurries compared to undigested slurries
does not guar...
Centrifugal Flotation Applied to the Separation of Oil and Fat from Wastewater
Engineering in Life Sciences - Tập 3 Số 2 - Trang 61-65 - 2003
New flotation devices such as flotation cyclones and flotation centrifuges have
successfully been demonstrated for the beneficiation of coal. In this project a
flotation centrifuge developed for that purpose was modified and applied to the
treatment of wastewater from a food processing plant containing emulgated oil
and fat. The cleaning efficiency of the flotation centrifuge was determined by
the...
bletl ‐ A Python package for integrating BioLector microcultivation devices in the Design‐Build‐Test‐Learn cycle
Engineering in Life Sciences - Tập 22 Số 3-4 - Trang 242-259 - 2022
AbstractMicrobioreactor (MBR) devices have emerged as powerful cultivation tools
for tasks of microbial phenotyping and bioprocess characterization and provide a
wealth of online process data in a highly parallelized manner. Such datasets are
difficult to interpret in short time by manual workflows. In this study, we
present the Python package bletl and show how it enables robust data analyses
and...
Influence of carbon and nitrogen concentration on itaconic acid production by the smut fungus Ustilago maydis
Engineering in Life Sciences - Tập 14 Số 2 - Trang 129-134 - 2014
Itaconic acid is a valuable platform compound for the production of bio‐based
polymers, chemicals, and fuels. Ustilago maydis is a promising host for the
production of itaconic acid from biomass‐derived substrates due to its
unicellular growth pattern and its potential to utilize biomass‐derived sugar
monomers and polymers. The potential of U. maydis for industrial itaconate
production was assesse...
Screening of bacterial strains capable of converting biodiesel‐derived raw glycerol into 1,3‐propanediol, 2,3‐butanediol and ethanol
Engineering in Life Sciences - Tập 12 Số 1 - Trang 57-68 - 2012
AbstractThe ability of bacterial strains to assimilate glycerol derived from
biodiesel facilities to produce metabolic compounds of importance for the food,
textile and chemical industry, such as 1,3‐propanediol (PD), 2,3‐butanediol (BD)
and ethanol (EtOH), was assessed. The screening of 84 bacterial strains was
performed using glycerol as carbon source. After initial trials, 12 strains were
ident...
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