Engineering in Life Sciences

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Analysis of intracellular metabolites of Corynebacterium glutamicum at high cell density with automated sampling and filtration and assessment of engineered enzymes for effective l‐lysine production
Engineering in Life Sciences - Tập 17 Số 5 - Trang 512-522 - 2017
Julian A. da Luz, Enrico Hans, Doinita Frank, An‐Ping Zeng
Engineering of enzymes and pathways is generally required for the development of efficient strains for bioproduction processes. To this end, quantitative and reliable data of intracellular metabolites are highly desired, but often not available, especially for conditions more close to industrial applications, i.e. at high cell density and product concentration. Here, we investigated the in...... hiện toàn bộ
Automated fast filtration and on‐filter quenching improve the intracellular metabolite analysis of microorganisms
Engineering in Life Sciences - Tập 14 Số 2 - Trang 135-142 - 2014
Julian A. da Luz, Enrico Hans, An‐Ping Zeng
To reliably determine intracellular metabolite concentrations in microorganisms, accurate sampling and sample inactivation strategies are crucial. Here, we present a method for automated fast filtration and on‐filter quenching of microbial samples to overcome metabolite leakage induced by cold shock and significantly reduce the sampling and treatment time compared to manual filtration meth...... hiện toàn bộ
Improved electrocompetence and metabolic engineering of Clostridium pasteurianum reveals a new regulation pattern of glycerol fermentation
Engineering in Life Sciences - Tập 19 Số 6 - Trang 412-422 - 2019
Rebekka Schmitz, Wael Sabra, Philipp Arbter, Yaeseong Hong, Tyll Utesch, An‐Ping Zeng
AbstractClostridium pasteurianum produces industrially valuable chemicals such as n‐butanol and 1,3‐propanediol from fermentations of glycerol and glucose. Metabolic engineering for increased yields of selective compounds is not well established in this microorganism. In order to study carbon fluxes and to selec...... hiện toàn bộ
Metabolomic and kinetic investigations on the electricity‐aided production of butanol by Clostridium pasteurianum strains
Engineering in Life Sciences - Tập 21 Số 3-4 - Trang 181-195 - 2021
Philipp Arbter, Wael Sabra, Tyll Utesch, Yaeseong Hong, An‐Ping Zeng
AbstractIn this contribution, we studied the effect of electro‐fermentation on the butanol production of Clostridium pasteurianum strains by a targeted metabolomics approach. Two strains were examined: an electrocompetent wild type strain (R525) and a mutant strain (dhaB mutant) lacking formation of 1,3‐propanediol (PDO). The dhaB‐negativ...... hiện toàn bộ
A novel All‐in‐One electrolysis electrode and bioreactor enable better study of electrochemical effects and electricity‐aided bioprocesses
Engineering in Life Sciences - Tập 18 Số 8 - Trang 600-610 - 2018
Tyll Utesch, An‐Ping Zeng
AbstractAn autoclavable All‐in‐One electrolysis electrode in a rod shape assembly is developed as a new tool for bioelectrochemical systems and electricity‐aided bioprocesses. It can replace the classic two‐chamber bioelectrochemical system for electrolysis reactions, be inserted into conventional bioreactors and is easily adaptable as electrocatalytic surface or g...... hiện toàn bộ
Effects of anaerobic digestion on digestate nutrient availability and crop growth: A review
Engineering in Life Sciences - Tập 12 Số 3 - Trang 242-257 - 2012
Kurt Möller, Torsten Müller
Anaerobic digestion (AD) for biogas production leads to several changes in the composition of the resulting digestates compared to the original feedstock (ammonia content, pH, carbon to nitrogen ratio, etc.), which are relevant for the plant availability of macro‐ and...... hiện toàn bộ
Centrifugal Flotation Applied to the Separation of Oil and Fat from Wastewater
Engineering in Life Sciences - Tập 3 Số 2 - Trang 61-65 - 2003
Bernd Benker, A. Wollmann, M. Sievers
New flotation devices such as flotation cyclones and flotation centrifuges have successfully been demonstrated for the beneficiation of coal. In this project a flotation centrifuge developed for that purpose was modified and applied to the treatment of wastewater from a food processing plant containing emulgated oil and fat. The cleaning efficiency of the flotation centrifuge was determine...... hiện toàn bộ
bletl ‐ A Python package for integrating BioLector microcultivation devices in the Design‐Build‐Test‐Learn cycle
Engineering in Life Sciences - Tập 22 Số 3-4 - Trang 242-259 - 2022
Michael Osthege, Niklas Tenhaef, Rebecca Zyla, Carolin Müller, Johannes Hemmerich, Wolfgang Wiechert, Stephan Noack, Marco Oldiges
AbstractMicrobioreactor (MBR) devices have emerged as powerful cultivation tools for tasks of microbial phenotyping and bioprocess characterization and provide a wealth of online process data in a highly parallelized manner. Such datasets are difficult to interpret in short time by manual workflows. In this study, we present the Python package bletl and show how it...... hiện toàn bộ
Influence of carbon and nitrogen concentration on itaconic acid production by the smut fungus Ustilago maydis
Engineering in Life Sciences - Tập 14 Số 2 - Trang 129-134 - 2014
Nicole Maaßen, Monika Maasem Panakova, Nick Wierckx, Elena Geiser, Martín Zimmermann, Michael Bölker, Ulrich Klinner, Lars M. Blank
Itaconic acid is a valuable platform compound for the production of bio‐based polymers, chemicals, and fuels. Ustilago maydis is a promising host for the production of itaconic acid from biomass‐derived substrates due to its unicellular growth pattern and its potential to utilize biomass‐derived sugar monomers and polymers. The potential of U. maydis...... hiện toàn bộ
Screening of bacterial strains capable of converting biodiesel‐derived raw glycerol into 1,3‐propanediol, 2,3‐butanediol and ethanol
Engineering in Life Sciences - Tập 12 Số 1 - Trang 57-68 - 2012
Maria Metsoviti, Spiros Paramithiotis, Eleftherios H. Drosinos, Maria Galiotou‐Panayotou, George‐John E. Nychas, An‐Ping Zeng, Séraphim Papanikolaou
AbstractThe ability of bacterial strains to assimilate glycerol derived from biodiesel facilities to produce metabolic compounds of importance for the food, textile and chemical industry, such as 1,3‐propanediol (PD), 2,3‐butanediol (BD) and ethanol (EtOH), was assessed. The screening of 84 bacterial strains was performed using glycerol as carbon source. After init...... hiện toàn bộ
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