Engineering in Life Sciences

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Automated fast filtration and on‐filter quenching improve the intracellular metabolite analysis of microorganisms
Engineering in Life Sciences - Tập 14 Số 2 - Trang 135-142 - 2014
Julian A. da Luz, Enrico Hans, An‐Ping Zeng
To reliably determine intracellular metabolite concentrations in microorganisms, accurate sampling and sample inactivation strategies are crucial. Here, we present a method for automated fast filtration and on‐filter quenching of microbial samples to overcome metabolite leakage induced by cold shock and significantly reduce the sampling and treatment time compared to manual filtration meth...... hiện toàn bộ
Influence of carbon and nitrogen concentration on itaconic acid production by the smut fungus Ustilago maydis
Engineering in Life Sciences - Tập 14 Số 2 - Trang 129-134 - 2014
Nicole Maaßen, Monika Maasem Panakova, Nick Wierckx, Elena Geiser, Martín Zimmermann, Michael Bölker, Ulrich Klinner, Lars M. Blank
Itaconic acid is a valuable platform compound for the production of bio‐based polymers, chemicals, and fuels. Ustilago maydis is a promising host for the production of itaconic acid from biomass‐derived substrates due to its unicellular growth pattern and its potential to utilize biomass‐derived sugar monomers and polymers. The potential of U. maydis...... hiện toàn bộ
Screening of bacterial strains capable of converting biodiesel‐derived raw glycerol into 1,3‐propanediol, 2,3‐butanediol and ethanol
Engineering in Life Sciences - Tập 12 Số 1 - Trang 57-68 - 2012
Maria Metsoviti, Spiros Paramithiotis, Eleftherios H. Drosinos, Maria Galiotou‐Panayotou, George‐John E. Nychas, An‐Ping Zeng, Séraphim Papanikolaou
AbstractThe ability of bacterial strains to assimilate glycerol derived from biodiesel facilities to produce metabolic compounds of importance for the food, textile and chemical industry, such as 1,3‐propanediol (PD), 2,3‐butanediol (BD) and ethanol (EtOH), was assessed. The screening of 84 bacterial strains was performed using glycerol as carbon source. After init...... hiện toàn bộ
Population dynamics of methanogens during acidification of biogas fermenters fed with maize silage
Engineering in Life Sciences - Tập 10 Số 6 - Trang 496-508 - 2010
Bernhard Munk, Christoph Bauer, Andreas Gronauer, Michael Lebuhn
AbstractLimitations in the supply of essential trace elements for methanogenic Archaea can arise in biogas production from renewable resources in flow‐through systems particularly when no manure is added. Without compensating supplementation, primarily Co and Na+ can become limiting in long‐term mono‐digestion of maiz...... hiện toàn bộ
Removal of Heavy Metals from the Environment by Biosorption
Engineering in Life Sciences - Tập 4 Số 3 - Trang 219-232 - 2004
Maria Gavrilescu
AbstractThe pollution of the environment with toxic metals is a result of many human activities, such as mining and metallurgy, and the effects of these metals on the ecosystems are of large economic and public‐healthsignificance. This paper presents the features and advantages of the unconventional removal method of heavy metals – biosorption – as a part of biorem...... hiện toàn bộ
Production of Sophorolipids fromCandida bombicola ATCC 22214 Using Turkish Corn Oil and Honey
Engineering in Life Sciences - Tập 5 Số 4 - Trang 357-362 - 2005
Gülseren Pekin, Fazilet Vardar‐Sukan, N. Kosaric
Laccase production in solid‐state and submerged fermentation by Pleurotus ostreatus
Engineering in Life Sciences - Tập 9 Số 1 - Trang 45-52 - 2009
Sharmistha Mazumder, Soumendra K. Basu, Mina Mukherjee
AbstractA solid‐state fermentation (SSF) system for production of an industrially important enzyme laccase by Pleurotus ostreatus was developed by using potato dextrose yeast extract medium and polyurethane foam as a supporting material. The maximum laccase production in the SSF system was as high as 3×105 U/L. Additi...... hiện toàn bộ
Enhanced biogas yield from energy crops with rumen anaerobic fungi
Engineering in Life Sciences - Tập 12 Số 3 - Trang 343-351 - 2012
Jindřich Procházka, Jakub Mrázek, Lenka Štrosová, Kateřina Fliegerová, J. Zábranská, M. Dohányos
Anaerobic fungi (AF) are able to degrade crop substrates with higher efficiency than commonly used anaerobic bacteria. The aim of this study was to investigate ways of use of rumen AF to improve biogas production from energy crops under laboratory conditions. In this ...... hiện toàn bộ
Analysis of intracellular metabolites of Corynebacterium glutamicum at high cell density with automated sampling and filtration and assessment of engineered enzymes for effective l‐lysine production
Engineering in Life Sciences - Tập 17 Số 5 - Trang 512-522 - 2017
Julian A. da Luz, Enrico Hans, Doinita Frank, An‐Ping Zeng
Engineering of enzymes and pathways is generally required for the development of efficient strains for bioproduction processes. To this end, quantitative and reliable data of intracellular metabolites are highly desired, but often not available, especially for conditions more close to industrial applications, i.e. at high cell density and product concentration. Here, we investigated the in...... hiện toàn bộ
Improved electrocompetence and metabolic engineering of Clostridium pasteurianum reveals a new regulation pattern of glycerol fermentation
Engineering in Life Sciences - Tập 19 Số 6 - Trang 412-422 - 2019
Rebekka Schmitz, Wael Sabra, Philipp Arbter, Yaeseong Hong, Tyll Utesch, An‐Ping Zeng
AbstractClostridium pasteurianum produces industrially valuable chemicals such as n‐butanol and 1,3‐propanediol from fermentations of glycerol and glucose. Metabolic engineering for increased yields of selective compounds is not well established in this microorganism. In order to study carbon fluxes and to selec...... hiện toàn bộ
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