Influence of carbon and nitrogen concentration on itaconic acid production by the smut fungus Ustilago maydis

Engineering in Life Sciences - Tập 14 Số 2 - Trang 129-134 - 2014
Nicole Maaßen1, Monika Maasem Panakova1, Nick Wierckx1, Elena Geiser1, Martín Zimmermann1, Michael Bölker2, Ulrich Klinner1, Lars M. Blank1
1Institute of Applied Microbiology, RWTH Aachen University, Aachen, Germany
2Department of Biology, Philipps University Marburg, Marburg, Germany

Tóm tắt

Itaconic acid is a valuable platform compound for the production of bio‐based polymers, chemicals, and fuels. Ustilago maydis is a promising host for the production of itaconic acid from biomass‐derived substrates due to its unicellular growth pattern and its potential to utilize biomass‐derived sugar monomers and polymers. The potential of U. maydis for industrial itaconate production was assessed in pH‐controlled batch fermentations with varying medium compositions. Using 200 g/L glucose and 75 mM ammonium, 44.5 g/L of itaconate was produced at a maximum rate of 0.74 g L−1 h−1. By decreasing the substrate concentrations to 50 g/L glucose and 30 mM ammonium, a yield of 0.34 g/g (47 mol%) could be achieved. Itaconate production from xylose was also feasible. These results indicate that high itaconic acid titers can be achieved with U. maydis. However, further optimization of the biocatalyst itself through metabolic engineering is still needed in order to achieve an economically feasible process, which can be used to advance the development of a bio‐based economy.

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Thông tin xuất bản

Engineering in Life Sciences

Tập 14 Số 2

129-134

Thông tin tác giả