Population dynamics of methanogens during acidification of biogas fermenters fed with maize silage

Engineering in Life Sciences - Tập 10 Số 6 - Trang 496-508 - 2010
Bernhard Munk1, Christoph Bauer1, Andreas Gronauer1, Michael Lebuhn1
1Bavarian State Research Center of Agriculture, Institute for Agricultural Engineering and Animal Husbandry, Freising, Germany

Tóm tắt

AbstractLimitations in the supply of essential trace elements for methanogenic Archaea can arise in biogas production from renewable resources in flow‐through systems particularly when no manure is added. Without compensating supplementation, primarily Co and Na+ can become limiting in long‐term mono‐digestion of maize silage at threshold values of ca. 0.03 and 10 mg/L, respectively. These deficiencies apparently triggered process acidification. Using molecular biological methods (PCR‐SSCP and quantitative real‐time PCR), microbial population dynamics were monitored qualitatively and quantitatively at distinct stages during the experiments, investigating mcrA/mrtA, coding for a subunit of the key enzyme of methanogenesis. An exponential correlation between mcrA/mrtA copies and methane productivity was obtained. Members of obligatley acetoclastic Methanosaetaceae were found only at low acetate concentrations (below 1 g/L). At organic loading rates>1 g volatile solids/(L×d) and without acidification symptoms, obligately hydrogenotrophic (oh) Methanobacteriales and versatile Methanosarcinaceae were dominating, and an abundance of up to 1010 methanogens per mL fermenter content was determined. In the acidified process, however, ca. 4 orders of magnitude less methanogens were detected, and Methanomicrobiales (oh), more specifically Methanospirillum hungatei or Methanoculleus spp., were dominating. Species diversity at the DNA level was highest at efficient process performance without stress symptoms and at a relatively low organic loading rate (1–2 g volatile solids/(L×d)). According to the quantitative real‐time PCR data, the process was not sustained below an availability of 10−8 to 10−9 μg Co per methanogenic cell.

Từ khóa


Tài liệu tham khảo

10.1007/BF00871639

10.1111/j.1574-6976.1999.tb00390.x

10.1016/S1369-703X(98)00043-6

10.1007/s00253-009-2246-7

10.2166/wst.2008.495

10.1099/00221287-144-9-2377

10.1099/00221287-148-11-3521

10.2166/wst.2004.0065

10.1128/AEM.59.8.2657-2665.1993

10.1128/aem.63.10.3741-3751.1997

Lebuhn M., 2003, Using quantitative real‐time PCR to determine the hygienic status of cattle manure, Water Sci. Technol., 48, 97, 10.2166/wst.2003.0230

10.1016/j.watres.2009.03.019

Garcés G. Effenberger M. Najdrowski M. Gronauer A.et al. Quantitative real‐time PCR for detectingCryptosporidium parvumin cattle manure and anaerobic digester samples – Methodological advances in DNA extraction Proceedings of the 8th Latin America Workshop Symposium on Anaerobic Digestion Punta del Este Uruguay 2005 68−73 ISBN 9974‐7699‐3‐0

10.2166/wst.2008.514

10.1002/cbic.200300662

10.1111/j.1574-6941.1999.tb00616.x

10.1111/j.1574-6941.2007.00331.x

10.1007/s00253-009-1912-0

10.1093/molbev/msm092

10.1016/0003-2697(91)90120-I

10.1111/j.1574-6976.2000.tb00564.x

10.1111/j.1574-6941.2006.00105.x

Hammer Ø., 2001, PAST: palaeontological statistics software package for education and data analysis, Palaeontol. Electronica., 4, 9

10.1038/sj.jim.2900546

10.1016/S0961-9534(97)00015-9

10.1016/j.biortech.2009.08.076

10.1016/j.watres.2010.09.001

10.1016/S0014-5793(99)01026-1

10.2166/wst.2005.0503

10.2166/wst.2005.0502

10.1016/j.biortech.2007.11.024

10.1128/AEM.70.11.6559-6568.2004

10.1128/AEM.02858-08

10.1111/j.1574-6968.1992.tb04987.x

10.1128/AEM.00489-06

10.2307/3546587

10.1016/j.syapm.2008.02.003

10.1016/j.jbiotec.2008.05.008

10.1007/s11157-008-9131-1

10.1016/j.biortech.2003.11.005

Thông tin
Thông tin xuất bản

Engineering in Life Sciences

Tập 10 Số 6

496-508

Thông tin tác giả