Developmental Dynamics
1058-8388
1097-0177
Mỹ
Cơ quản chủ quản: WILEY , Wiley-Liss Inc.
Lĩnh vực:
Developmental Biology
Phân tích ảnh hưởng
Thông tin về tạp chí
Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.
Các bài báo tiêu biểu
The origin of the diversity of leaf venation pattern Abstract The leaf venation pattern of plants shows remarkable diversity and species‐specificity. However, the mechanism underlying the pattern formation and pattern diversity remains unclear. We developed a mathematical model that is based on the positive feedback regulation between plant hormone auxin and its efflux carrier. This system can generate auxin flow pathways by self‐organization from an almost homogeneous state. This result explains a well‐known experimental phenomenon referred as to “polar auxin transport.” The model can produce diverse leaf venation patterns with spatial regularity under similar conditions to those of leaf development, that is, in the presence of leaf expansion and auxin sink. Final venation patterns are strikingly affected by leaf shape and leaf expansion. These results indicate that the positive feedback regulation between auxin and its efflux carrier is a central dynamic in leaf venation pattern formation. The diversity of leaf venation patterns in plant species is probably due to the differences of leaf shape and leaf expansion pattern. Developmental Dynamics 235:2710–2721, 2006. © 2006 Wiley‐Liss, Inc.
Tập 235 Số 10 - Trang 2710-2721 - 2006
Histone deacetylase 1 and 2 are essential for murine neural crest proliferation, pharyngeal arch development, and craniofacial morphogenesis Background :Results: Cdkn1a, Cdkn1b, Cdkn1c, Cdkn2b, Cdkn2c , and Tp53 within the first pharyngeal arch. Conclusions: Developmental Dynamics 246:1015–1026, 2017 . © 2017 Wiley Periodicals, Inc.
Tập 246 Số 12 - Trang 1015-1026 - 2017
Phenotypic analysis of <i>deflated</i>/Ints7 function in <i>Drosophila</i> development Abstract The Drosophila gene deflated (CG18176; renamed after the pupal lethal abdominal phenotype of mutant individuals) is a member of a conserved gene family found in all multicellular organisms. The human orthologue of deflated (Ints7) encodes a subunit of the Integrator complex that associates with RNA polymerase II and has been implicated in snRNA processing. Since loss‐of‐function analyses of deflated have not yet been reported, we undertook to investigate deflated expression patterns and mutant phenotypes. deflated mRNA was detected at low levels in proliferating cells in postblastoderm embryos and GFP tagged protein is predominately nuclear. Generation and analysis of four mutant alleles revealed deflated is essential for normal development, as mutant individuals displayed pleiotropic defects affecting many stages of development, consistent with perturbation of cell signalling or cell proliferation. Our data demonstrate multiple roles in development for an Ints7 homologue and to demonstrate its requirement for normal cell signalling and proliferation. Developmental Dynamics 238:1131–1139, 2009. © 2009 Wiley‐Liss, Inc.
Tập 238 Số 5 - Trang 1131-1139 - 2009
<i>Gsh</i>‐<i>1</i>: A novel murine homeobox gene expressed in the central nervous system Abstract We report the characterization of Gsh ‐1 , a novel murine homeobox gene. Northern blot analysis revealed a transcript of approximately 2 kb in size present at embryonic days 10.5, 11.5, and 12.5 of development. The cDNA sequence encoded a proline rich motif, a polyalanine tract, and a homeodomain with strong homology to those encoded by the clustered Hox genes. The Gsh ‐1 expression pattern was determined for days E8.5 to E13.5 by whole mount and serial section in situ hybridizations. Gsh ‐1 transcription was restricted to the central nervous system. Expression is present in the neural tube and hindbrain as two continuous, bilaterally symmetrical stripes within neural epithelial tissue. In the mesencephalon, expression is seen as a band across the most anterior portion. There is also diencephalon expression in the anlagen of the thalamus and the hypothalamus as well as in the optic stalk, optic recess, and the ganglionic eminence. Moreover, through the use of fusion proteins containing the Gsh ‐1 homeodomain, we have determined the consensus DNA ninding site of the Gsh ‐1 homeoprotein to be GCT /C A /C ATTA G /A . ©1995 Wiley‐Liss, Inc.
Tập 203 Số 3 - Trang 337-351 - 1995
Expression of homeobox genes <i>Msx‐1 (Hox‐7)</i> and <i>Msx‐2 (Hox‐8)</i> during cardiac development in the chick Abstract The vertebrate homeobox genes Msx ‐1 and Msx ‐2 are related to the Drosophila msh gene and are expressed in a variety of tissues during embryogenesis. We have examined their expression by in situ hybridisation during critical stages of cardiac development in the chick from stages 15 + to 37. Msx ‐1 expression is apparent in a number of non‐myocardial cell populations, including cells undergoing an epithelial to mesenchymal transformation in the atrioventricular and the outflow tract regions that play an integral role in heart septation and valve formation. Msx.2 expression is restricted to a distinct subpopulation of myocardial cells that, in later stages, coincides morphologically with the cardiac conduction system. The timing of Msx ‐2 expression suggests that it plays a role in conduction system tissue formation and that it identifies precursor cells of this specialised myocardium. The pattern of Msx ‐2 expression is discussed with reference to current models of conduction tissue development. © 1993 Wiley‐Liss, Inc.
Tập 197 Số 3 - Trang 203-216 - 1993
Experimental analysis of <i>Msx‐1</i> and <i>Msx‐2</i> gene expression during chick mandibular morphogenesis Abstract Homeobox‐containing genes are thought to be involved in regulating pattern formation in a variety of tissues during embryogenesis. We have examined the expression of the homeobox‐related genes Msx ‐1 and Msx ‐2 during the development of the chick mandibular arch. Northern blot hybridization indicates that transcripts for both Msx ‐1 (1.6 Kb) and Msx ‐2 (3 Kb) are present in the mandibular arch as early as stage 18. The levels of both transcripts in the whole mandible decrease as cartilage is formed in vivo and in vitro. Using in situ hybridization, transcripts of Msx ‐1 were localized in high amounts to the mesenchyme of the mesial tips of the arches. Msx ‐2 transcripts were localized in high amounts to medial regions of the arches. Little or no hybridization of either probe was detected in the chondrogenic and myogenic regions of the arches. Transcripts of both genes were also excluded from calcified bone and cartilage. Our results further demonstrate that the mesial tip mesenchyme expressing Msx ‐1 includes areas of highly proliferative cells and has in vitro chondrogenic potential. The region of mesenchymal cells expressing the Msx ‐2 gene overlap with areas of developmentally programmed cell death which also contain very few proliferative cells and lack chondrogenic potential in vitro. These results are consistent with the possibility that Msx ‐1 may be involved in the outgrowth of the mandibular arch and Msx ‐2 may be involved in both developmentally programmed cell death and delineating the non‐chondrogenic region of the medial part of the mandibular arch. © 1995 Wiley‐Liss, Inc.
Tập 202 Số 2 - Trang 195-214 - 1995
Differential expression of myogenic regulatory genes and Msx‐1 during dedifferentiation and redifferentiation of regenerating amphibian limbs Abstract An amputated limb of an adult urodele amphibian is capable of undergoing regeneration. The new structures form from an undifferentiated mass of cells called the regenerative blastema. The cells of the blastema are believed to derive from differentiated tissues of the adult limb. However, the exact source of these cells and the process by which they undergo dedifferentiation are poorly understood. In order to elucidate the molecular and cellular basis for dedifferentiation we isolated a number of genes which are potential regulators of the process. These include Msx ‐1 , which is believed to support the undifferentiated and proliferative state of cells in the embryonic limb bud; and two members of the myogenic regulatory gene family, MRF ‐4 and Myf ‐5 , which are expressed in differentiated muscle and regulate muscle‐specific gene activity. As anticipated, we find that Msx ‐1 is strongly up‐regulated during the initiation of regeneration. It remains expressed throughout regeneration but is not found in the fully regenerated limb. The myogenic gene MRF ‐4 has the reverse expression pattern. It is expressed in adult limb muscle, is rapidly shut off in early regenerative blastemas, and is only reexpressed at the completion of regeneration. These kinetics are paralleled by those of a musclespecific Myosin gene. In contrast Myf ‐5 , a second member of the myogenic gene family, continues to be expressed throughout the regenerative process. Thus, MRF ‐4 and Myf ‐5 are likely to play distinct roles during regeneration. MRF ‐4 may directly regulate muscle phenotype and as such its repression may be a key event in dedifferentiation. Myf ‐5 may play a role in maintaining a distinct myogenic lineage during regeneration. © 1995 Wiley‐Liss, Inc.
Tập 202 Số 1 - Trang 1-12 - 1995
The zebrafish, brain‐specific, aromatase <i>cyp19a2</i> is neither expressed nor distributed in a sexually dimorphic manner during sexual differentiation Abstract Differential cyp19 aromatase expression during development leads to sexual dimorphisms in the mammalian brain. Whether this is also true for fish is unknown. The aim of the current study has been to follow the expression of the brain‐specific aromatase cyp19a2 in the brains of sexually differentiating zebrafish. To assess the role of cyp19a2 in the zebrafish brain during gonadal differentiation, we used quantitative reverse transcriptase‐polymerase chain reaction and immunohistochemistry to detect differences in the transcript or protein levels and/or expression pattern in juvenile fish, histology to monitor the gonadal status, and double immunofluorescence with neuronal or radial glial markers to characterize aromatase‐positive cells. Our data show that cyp19a2 expression levels during zebrafish sexual differentiation cannot be assigned to a particular sex; the expression pattern in the brain is similar in both sexes and aromatase‐positive cells appear to be mostly of radial glial nature. Developmental Dynamics 236:3155–3166, 2007. © 2007 Wiley‐Liss, Inc.
Tập 236 Số 11 - Trang 3155-3166 - 2007
Retinoic acid, a regeneration‐inducing molecule Abstract Retinoic acid (RA) is the biologically active metabolite of vitamin A. It is a low molecular weight, lipophilic molecule that acts on the nucleus to induce gene transcription. In amphibians and mammals, it induces the regeneration of several tissues and organs and these examples are reviewed here. RA induces the “super‐regeneration” of organs that can already regenerate such as the urodele amphibian limb by respecifying positional information in the limb. In organs that cannot normally regenerate such as the adult mammalian lung, RA induces the complete regeneration of alveoli that have been destroyed by various noxious treatments. In the mammalian central nervous system (CNS), which is another tissue that cannot regenerate, RA does not induce neurite outgrowth as it does in the embryonic CNS, because one of the retinoic acid receptors, RARβ2, is not up‐regulated. When RARβ2 is transfected into the adult spinal cord in vitro, then neurite outgrowth is stimulated. In all these cases, RA is required for the development of the organ, in the first place suggesting that the same gene pathways are likely to be used for both development and regeneration. This suggestion, therefore, might serve as a strategy for identifying potential tissue or organ targets that have the capacity to be stimulated to regenerate. Developmental Dynamics 226:237–244, 2003.© 2003 Wiley‐Liss, Inc.
Tập 226 Số 2 - Trang 237-244 - 2003
Mash1 is required for the differentiation of AADC-positive type III cells in mouse taste buds
Tập 240 Số 4 - Trang 775-784 - 2011