Current Genetics
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Genetic transfer mediated by isolated nuclei in Saccharomyces cerevisiae
Current Genetics - Tập 6 - Trang 163-165 - 1982
Viable hybrids of Saccharomyces cerevisiae were obtained by transfer of isolated diploid nuclei into haploid protoplasts using a polyethylene glycol (PEG) fusion procedure.
A DNA sequence in Saccharomyces exiguus is homologous with the HO gene of Saccharomyces cerevisiae
Current Genetics - Tập 15 - Trang 399-401 - 1989
The DNA of Saccharomyces exiguus was analyzed by Southern hybridization using cloned MATa, MATα, and HO genes of Saccharomyces cerevisiae as probes. It was shown that S. exiguus has a DNA sequence homologous with the HO gene of S. cerevisiae and that this DNA sequence is on a chromosome of about 940 kb of DNA in S. exiguus. However, there is no DNA sequence in S. exiguus that is homologous with th...... hiện toàn bộ
A yeast strain producing lys2 deletions at a high frequency after sporulation
Current Genetics - Tập 14 - Trang 331-335 - 1988
A diploid yeast strain with extensive sequence dissimilarity in homologous regions near the LYS2 locus was sporulated, and spontaneous lys2 and lys5 mutant spores, selected on α-amino adipate, were analyzed. As many as 50% of the mutant spores contained a deletion in LYS2. These deletions occurred at a frequency of 5.0 × 10−7. While deletions of various sizes and endpoints were obtained, all the d...... hiện toàn bộ
The vacuolar morphology protein VAC14 plays an important role in sexual development in the filamentous ascomycete Sordaria macrosporaAbstract The multiprotein Fab1p/PIKfyve-complex regulating the abundance of the phospholipid phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2 ) is highly conserved among eukaryotes. In yeast/mammals, it is composed of the phosphatidylinositol 3-phosphate 5-kinase Fab1p/PIKfyve, the PtdIns(3,5)P2 phosphatase Fig4p/Sac3 and... ... hiện toàn bộ
Current Genetics - Tập 68 Số 3-4 - Trang 407-427 - 2022
Recurrent invasion of mitochondrial group II introns in specimens of Pylaiella littoralis (brown alga), collected worldwide
Current Genetics - Tập 53 - Trang 207-216 - 2008
The mitochondrial genome of a filamentous brown alga Pylaiella
littoralis (strain CCMP 1907) has been reported to contain four group IIB introns in the LSU rRNA gene and three group IIA introns in the cox1 gene. We found extreme variability in the number of group II introns for these two genes by analyzing eight P. littoralis specimens collected at worldwide habitats. The f...... hiện toàn bộ
Antisuppressor mutations reduce misreading of the genetic code in Aspergillus nidulans
Current Genetics - Tập 19 - Trang 461-465 - 1991
Antisuppressor mutations were isolated in a strain containing the omnipotent suppressor suaC109. The antisuppressors reduce the activity of translational suppressors in vivo and counteract most aspects of the pleiotropic phenotype associated with the suaC and the suaA suppressor mutations. Using an homologous system for cell-free translation, we have measured translational accuracy in two antisupp...... hiện toàn bộ
Homologous transformation of the lignin-degrading basidiomycete Phanerochaete chrysosporium
Current Genetics - Tập 19 Số 6 - Trang 491-494 - 1991
Nonrandomly-associated forward mutation and mitotic recombination yield yeast diploids homozygous for recessive mutations
Current Genetics - Tập 26 - Trang 302-307 - 1994
We have employed the analysis of spontaneous forward mutations that confer the ability to utilize L-α-aminoadipate as a nitrogen source (α-Aa+) to discern the events that contribute to mitotic segregation of spontaneous recessive mutations by diploid cells. α-Aa- diploid cells yield α-Aa+ mutants at a rate of 7.8±3.6×10-9. As in haploid strains, approximately 97% (30/31) of α-Aa+ mutants are spont...... hiện toàn bộ
Molecular characterization and cloning of sheep mitochondrial DNA
Current Genetics - Tập 21 Số 3 - Trang 235-240 - 1992
The devil is in the details: comparison between COP9 signalosome (CSN) and the LID of the 26S proteasome
Current Genetics - Tập 62 - Trang 129-136 - 2015
The COP9 signalosome (CSN) and the proteasomal LID are conserved macromolecular complexes composed of at least eight subunits with molecular weights of approximately 350 kDa. CSN and LID are part of the ubiquitin–proteasome pathway and cleave isopeptide linkages of lysine side chains on target proteins. CSN cleaves the isopeptide bond of ubiquitin-like protein Nedd8 from cullins, whereas the LID c...... hiện toàn bộ
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