Molecular analysis of a leu2 − mutant of Candida maltosa demonstrates the presence of multiple alleles

Current Genetics - Tập 26 - Trang 208-216 - 1994
Dietmar Becher1, Steffen Schulze1, Anette Kasüske1, Heiko Schulze1, Ida A. Samsonova1, Stephen G. Oliver2
1Fachrichtung Biologie, Institut für Genetik und Biochemie, Ernst-Moritz-Arndt-Universität Greifswald, Greifswald, Germany
2Department of Biochemistry and Applied Molecular Biology, UMIST, Manchester, UK

Tóm tắt

Three different alleles of the β-isopropylmalate dehydrogenase gene were cloned and sequenced from a leucine auxotrophic mutant, G587, of Candida maltosa. The cloning of functionally-intact wild-type genes from this mutant strain suggests the presence of silent gene copies. An interallelic-divergence comparison has provided evidence for new regulatory mechanisms. Sequence data and karyotype analysis argue for a highly-aneuploid genome of C. maltosa. An interpretation for the spontaneous auxotrophy-prototrophy-auxotrophy sequence of mutations in C. maltosa is suggested.

Tài liệu tham khảo

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