Identification of quantitative trait loci affecting virulence in the basidiomycete Heterobasidion annosum s.l.
Tóm tắt
Identification of virulence factors of phytopathogens is important for the fundamental understanding of infection and disease progress in plants and for the development of control strategies. We have identified quantitative trait loci (QTL) for virulence on 1-year-old Pinus sylvestris and 2-year-old Picea abies seedlings and positioned them on a genetic linkage map of the necrotrophic phytopathogen Heterobasidion annosum sensu lato (s.l.), a major root rot pathogen on conifers. The virulence of 102 progeny isolates was analysed using two measurements: lesion lengths and fungal growth in sapwood from a cambial infection site. We found negative virulence effects of hybridization although this was contradicted on a winter-hardened spruce. On P. abies, both measurements identified several partially overlapping QTLs on linkage group (LG) 15 of significant logarithm of odds (LOD) values ranging from 2.31 to 3.85. On P. sylvestris, the lesion length measurement also identified a QTL (LOD 3.09) on LG 15. Moreover, QTLs on two separate smaller LGs, with peak LOD values of 2.78 and 4.58 were identified for fungal sapwood growth and lesion lengths, respectively. The QTL probably represent loci important for specific as well as general aspects of virulence on P. sylvestris and P. abies.
Tài liệu tham khảo
Adomas A, Asiegbu FO (2007) Analysis of organ-specific responses of Pinus sylvestris to shoot (Gremmeniella abietina) and root (Heterobasidion annosum) pathogens. Physiol Mol Plant Pathol. doi:10.1016/j.pmpp.2007.04.001
Albersheim P, Anderson-Prouty A (1975) Carbohydrates, proteins, cell surfaces and the biochemistry of pathogenesis. Annu Rev Plant Physiol 26:31–52
Anderson M, Kasuga T, Mitchelson K (1993) A partial physical karyotype of Heterobasidion annosum. In: Johansson M, Stenlid J (eds) Eighth international conference on root and butt rots. Wik, Sweden and Haikko, Finland
Asiegbu FO (2000) Adhesion and development of the root rot fungus (Heterobasidion annosum) on conifer tissues: effects of spore and host surface constituents. FEMS Microbiol 33:101–110
Asiegbu FO, Daniel G, Johansson M (1994) Defence related reactions of seedling roots of Norway spruce to infection by Heterobasidion annosum (Fr) Bref. Physiol Mol Plant Pathol 45:1–19
Asiegbu FO, Johansson M, Woodward S, Hüttermann A (1998) Biochemistry in the host–parasite interaction. In: Woodward S, Stenlid J, Karjalainen R, Hüttermann A (eds) Heterobasidion annosum; biology, ecology, impact and control. CAB International, Wallingford, pp 167–193
Asiegbu FO, Adomas A, Stenlid J (2005) Conifer root and butt rot caused by Heterobasidion annosum (Fr) Bref. s. l. Mol Plant Pathol 6:395–409
Atkinson M (1993) Molecular mechanisms of pathogen recognition by plants. Adv Plant Pathol 10:35–64
Capretti P, Korhonen K, Mugnai L, Romagnoli C (1990) An intersterility group of Heterobasidion annosum, specialized to Abies alba. Eur J Forest Pathol 20:231–240
Chase TE (1985) PhD Thesis, University of Vermont, Burlington
Cumagun CJR, Bowden R, Jurgenson J, Leslie J, Miedaner T (2004) Genetic mapping of pathogenicity and aggressiveness of Gibberella zeae (Fusarium graminearum) toward wheat. Phytopathology 94:520–526
Dangl JL, Jones JD (2001) Plant pathogens and integrated defence responses to infection. Nature 411:826–833
Falconer D (1989) Introduction to quantitative genetics, 3rd. Longman Group Limited, London
Flor H (1971) Current status of the gene for gene concept. Annu Rev Phytopathol 9:275–296
Garbelotto M, Ratcliff A, Bruns TD, Cobb FW, Otrosina WJ (1996) Use of Taxon-specific competitive-priming PCR to study host specificity, hybridization, and intergroup gene flow in intersterility groups of Heterobasidion annosum. Phytopathology 86:543–551
Garbelotto M, Otrosina WJ Chapela IH, Gonthier P (2001) Studies on the ecology and genetics of hybridization in Heterobasidion. In: Laflamme G, Bérubé JA, Bussières G (eds) 10th international conference on root and butt rots, pp 238–244
Garbelotto M, Gonthier P, Nicolotti G (2004) Assessing fitness of Heterobasidion F1 hybrids through inoculation experiments. In: Manka M, Lakomy P (eds) 11th international conference on root and butt rots, pp 255–260
Gilchrist D (1998) Programmed cell death in plant disease: the purpose and promise of cellular suicide. Annu Rev Phytopathol 36:393–414
Greenberg J, Yao N (2004) The role and regulation of programmed cell death in plant–pathogen interactions. Cell Microbiol 6:201–211
Harrington T, Worrall JJ, Rizzo D (1989) Compatibility among host-specialized isolates of Heterobasidion annosum from western North America. Phytopathology 79:290–296
Hawthorne B, Rees-George J, Bowen J, Ball R (1997) A single locus with a large effect on virulence in Nectria haematococca MPI. Fungal Genet Newsl 44:24–26
Heath M (2000) Hypersensitive response-related death. Plant Mol Biol 44:321–334
Holz G, Coertze S, Williamson B (2004) The ecology of Botrytis on plant surfaces. In: Elad Y, Williamson B, Tudzynski P, Delen N (eds) Botrytis: biology, pathology and control. Kluwer, Dordrecht, pp 9–27
Jiang R, Weide R, de Vondervoort P, Govers F (2006) Amplification generates modular diversity at an avirulence locus in the pathogen Phytophthora. Genome Res 16:827–840
Johansson M (1988) Pectic enzyme activity of spruce (S) and pine (P) strains of Heterobasidion annosum (Fr) Bref. Physiol Mol Plant Pathol 33:333–349
Johansson M, Lundgren L, Asiegbu FO (1994) Initial interactions in living bark and sapwood of conifers infected by root rot fungi. In: Aamlid D (ed) Proceedings from the SNS meetings in forest pathology. Biri, Norway, pp 12–16
Johansson M, Denekamp M, Asiegbu FO (1999) Production and isozyme pattern of extracellular laccase in the S and P intersterility groups of the root pathogen Heterobasidion annosum. Mycol Res 103:365–371
Jones E (1994) Fungal adhesion. Mycol Res 98:961–981
Karlsson J, Stenlid J (1991) Pectic isozyme profiles of the intersterility groups in Heterobasidion annosum. Mycol Res 95:531–536
Karlsson M, Olson Å, Stenlid J (2003) Expressed sequences from the basidiomycetous tree pathogen Heterobasidion annosum during early infection of scots pine. Fungal Genet Biol 39:51–59
Karlsson M, Stenlid J, Olson Å (2005) Identification of a superoxide dismutase gene from the conifer pathogen Heterobasidion annosum. Physiol Mol Plant Pathol 66:99–107
Knoche H, Duvick J (1987) The role of fungal toxins in plant disease. In: Pegg G, Ayres P (eds) Fungal infection of plants. Cambridge University Press, Cambridge, pp 158–191
Korhonen K (1978) Intersterility groups of Heterobasidion annosum. Comm Inst For Fenn 94:1–25
Korhonen K, Stenlid J (1998) Biology of Heterobasidion annosum. In: Woodward S, Stenlid J, Karjalainen R, Hüttermann A (eds) Heterobasidion annosum: biology, ecology, impact and control. CAB International. Wallingford, pp 43–70
Kubisiak TL, Amerson HV, Nelson CD (2005) Genetic interaction of the fusiform rust fungus with resistance gene Fr1 in loblolly pine. Phytopathology 95:376–380
Lander ES, Botstein D (1988) Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199
Larraya LM, Idareta E, Arana D, Ritter E, Pisabarro AG, Ramirez L (2002) Quantitative trait loci controlling vegetative growth rate in the edible Basidiomycete Pleurotus ostreatus. Appl Environ Microbiol 68:1109–1114
Larraya LM, Alfonso M, Pisabarro AG, Ramirez L (2003) Mapping of genomic regions (quantitative trait loci) controlling production and quality in industrial cultures of the edible Basidiomycete Pleurotus ostreatus. Appl Environ Microbiol 69:3617–3625
Lind M, Olson Å, Stenlid J (2005) An AFLP-marker based genetic linkage map of Heterobasidion annosum locating intersterility genes. Fungal Genet Biol 42:519–527
Lindberg M, Johansson M (1991) Growth of Heterobasidion annosum through bark of Picea abies. Eur J Forest Pathol 21:377–388
Lyon GD, Goodman BA, Williamson B (2004) Botrytis cinerea perturbs redox strategies as an attack strategy in plants. In: Elad Y, Williamson B, Tudzynski P, Delen N (eds) Botrytis: biology, pathology and control. Kluwer, Dordrecht, pp 119–141
Manocha M (1984) Cell surface characteristics of Mortierella and their interaction with a mycoparasite. Can J Microbiol 30:290–298
Moquet F, Desmerger C, Mamoun M, Ramos-Guedes-Lafargue M, Olivier J-M (1999) A quantitative trait locus of Agaricus bisporus resistance to Pseudomonas tolaasii is closely linked to natural cap color. Fungal Genet Biol 28:34–42
Morel JB, Dangl J (1997) The hypersensitive response and the induction of cell death in plants. Cell Death Diff 4:671–683
Niemelä T, Korhonen K (1998) Taxonomy of the genus Heterobasidion. In: Woodward S, Stenlid J, Karjalainen R, Hüttermann A (eds) Heterobasidion annosum: biology, ecology, impact and control. CAB International. Wallingford, pp 27–33
Olson Å (2006) Genetic linkage between growth rate and the intersterility genes S and P in the basidiomycete Heterobasidion annosum s.l. Mycol Res 110:979–984
Olson Å, Stenlid J (2001) Mitochondrial control of fungal hybrid virulence. Nature 411:438
Olson Å, Lind M, Stenlid J (2005) In vitro test of virulence in the progeny of a Heterobasidion interspecific cross. For Pathol 35:321–331
Samils N, Elfstrand M, Lindner Czederpiltz D L, Fahleson J, Olson Å, Dixelius C, Stenlid S (2006) Development of a rapid and simple Agrobacterium tumefaciens-mediated transformation system for the fungal pathogen Heterobasidion annosum. FEMS Microbiol Lett 255:82–88
Siegee D (1993) Bacterial plant pathology; cell and molecular aspects. Cambridge University Press, Cambridge, pp 126–171
Sierotzki H, Gessler C (1998) Genetic analysis of a cross of two Venturia inaequalis strains that differ in virulence. J Phytopathol 146:515–519
Soanes DM, Talbot NJ (2006) Comparative genomic analysis of phytopathogenic fungi using expressed sequence tag (EST) collections. Mol Plant Pathol 7:61–71
Stenlid J (1985) Population structure of Heterobasidion annosum as determined by somatic incompatibility, sexual incompatibility and isoenzyme patterns. Can J Bot 63:2268–2273
Stenlid J, Karlsson J (1991) Partial intersterility in Heterobasidion annosum. Mycol Res 95:1153–1159
Swedjemark G, Johannesson H, Stenlid J (1999) Intraspecific variation in Heterobasidion annosum for growth in sapwood of Picea abies and Pinus silvestris. Eur J Forest Pathol 29:249–258
Swedjemark G, Stenlid J, Karlsson B (2001) Variation in growth of Heterobasidion annosum among clones of Picea abies incubated for different periods of time. For Pathol 31:163–175
Thrall PH, Burdon JJ (2003) Evolution of virulence in a plant host–pathogen metapopulation. Science 299:1735–1737
Van Ooijen J, Boer MP, Jansen RC, Maliepaard C (2002) MapQTL 4.0, Software for the calculation of QTL positions on genetic maps. Plant Research International, Wageningen, the Neterlands
Vinatzer B, Patocchi A, Gianfranceschi L, Tartarini S, Zhang H, Gessler C, Sansavini S (2001) Apple contains receptor-like genes homologous to the Cladosporium fulvum resistance gene family of tomato with a cluster of genes cosegregating with Vf apple scab resistance. Mol Plant Microbe Interact 14:508–515
Welz H, Leonard K (1994) Genetic analysis of two race 0 X race 2 crosses in Cochliobolus carbonum. Phytopathology 84:83–91
Wilcox PL, Amerson HV, Kuhlman EG, Liu BH, OMalley DM, Sederoff RR (1996) Detection of a major gene for resistance to fusiform rust disease in loblolly pine by genomic mapping. PNAS 93:3859–3864
Wolpert T, Dunkle L, Ciuffetti LM (2002) Host-selective toxins and avirulence determinants: What’s in a name? Annu Rev Phytopathol 40:251–285
Woodward S, Stenlid J, Karjalainen R, Hûttermann A (1998) Heterobasidion annosum. Biology, ecology, impact and control. CAB International, Cambridge
Zhan J, Mundt CC, Hoffer ME, McDonald BA (2002) Local adaptation and effect of host genotype on the rate of pathogen evolution: an experimental test in a plant pathosystem. J Evol Biol 15:634–647
Zhong S, Steffenson BJ (2002) Identification and characterization of DNA markers associated with a locus conferring virulence on barley in the plant pathogenic fungus Cochliobolus sativus. Theor Appl Genet 104:1049–1054